Mucispirillum schaedleri Antagonizes Salmonella Virulence to Protect Mice against Colitis

Herp, Simone; Brugiroux, Sandrine; Garzetti, Debora; Ring, Diana; Jochum, Lara; Beutler, Markus; Eberl, Claudia; Hussain, Saib; Walter, Steffi; Gerlach, Roman G.; Ruscheweyh, Hans‐Joachim; Huson, Daniel H.; Sellin, Mikael E.; Slack, Emma; Hanson, Buck; Loy, Alexander; Baines, John F.; Rausch, Philipp; Basic, Marijana; Berry, David; Stecher, Bärbel

doi:10.1016/j.chom.2019.03.004

Cited by 241 publications

(190 citation statements)

References 84 publications

(99 reference statements)

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“…M schaedleri had been reported to be a mucus inhabitant and was widely associated with intestinal inflammation . A recent study revealed a high prevalence of M schaedleri in human mucosal biopsy samples, further suggestive of the potential important role of M schaedleri in human diseases. Here, we demonstrated the presence of M schaedleri in livers of mice with isolated steatosis and NASH.…”

Section: Discussionmentioning

confidence: 96%

Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis

Qian

Yao

et al. 2019

Liver International

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Background & Aims Nonalcoholic fatty liver disease encompasses isolated steatosis or nonalcoholic fatty liver and nonalcoholic steatohepatitis (NASH). NASH develops from isolated steatosis with obscure driving forces. We aim to identify key factors promoting this transition. Methods Following 21‐week of high‐fat diet feeding, obese mice were classified into two groups termed as isolated steatosis and NASH based on hematoxylin‐eosin staining of liver histology. The integrated multi‐omics analysis of lipidome, transcriptome and gut microbiome were performed in mice with isolated steatosis and NASH, and confirmed in human samples. Results Livers in mice with NASH lost most lipids, and the transcriptional landscape was also changed dramatically in mice with NASH in relative to mice with isolated steatosis. Plasma lipidome analysis demonstrated a very clear difference between these two groups of mice, which was partially recapitulated in serum of patients with isolated steatosis and NASH. The microbiota composition revealed that Bacteroides genus and Bacteroides uniformis species decreased while Mucispirillum genus and Mucispirillum schaedleri species increased largely in mice with NASH. More importantly, we found that Bacteroides uniformis correlated positively with triglycerides (TGs) and negatively with free fatty acids (FFAs) and PE(18:1/20:4), while Mucispirillum schaedleri correlated positively with FFAs, LysoPC(20:3), LysoPC(20:4) and DG(16:1/18:2). Mechanically, administration of Bacteroides uniformis increased specific TGs, and decreased hepatic injury and inflammation in diet‐induced mice. Conclusions Overall, through multi‐omics integration, we identified a microbiota‐lipid axis promoting the initiation of NASH from isolated steatosis, which might provide a novel perspective on NASH pathogenesis and treatment.

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Section: Discussionmentioning

confidence: 96%

Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis

Qian

Yao

et al. 2019

Liver International

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“…Additionally, in male mice, DHA selectively increased butyrate‐producing Coprococcus [ 47 ] while suppressed pro‐inflammatory species Streptococcus [ 48 ] and p‐75‐a5 which were strongly correlated with HOMA‐IR and LPS production in our study. EPA selectively enriched the abundances of Mucispirillum schaedleri [ 49 ] and Barnesiella [ 50 ] which have been suggested to counteract colitis in mice and elevated S24‐7 [ 51 ] and Barnesiella [ 52 ] linked with ameliorated glucose metabolism, whereas substantially abolished SCFA‐producing Clostridium XlVa [ 53 ] which might explain the unchanged levels of propionate and butyrate in male mice. In female mice, DHA specifically decreased the abundance of pro‐inflammatory Enterorhabdus [ 54 ] and promoted the growth of butyrate‐producing Intestinimonas [ 55 ] and Bacteroides acidifaciens [ 56 ] which were shown to prevent obesity and improve insulin sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Eicosapentaenoic and Docosahexaenoic Acids Differentially Alter Gut Microbiome and Reverse High‐Fat Diet–Induced Insulin Resistance

Zhuang

Zhang

Shou

et al. 2020

Molecular Nutrition Food Res

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Scope: To assess the individual effects of dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on insulin resistance (IR), gut microbiome, and gut metabolites in high-fat-diet-induced obese (DIO) mice. Methods and results: DIO mice are fed an either high-fat diet (HFD), EPA (1% w/w) enriched HFD, or DHA (1% wt/wt) enriched HFD for 15 weeks. Both EPA and DHA supplements reverse hyperglycemia and IR but do not affect body weight in DIO mice while DHA exhibits a more pronounced ameliorative effect in male mice. Both EPA-and DHA-enriched Lactobacillus and short-chain fatty acids (SCFAs)-producing species from Lachnospiraceae while reduced lipopolysaccharide (LPS)-producing Bilophila and Escherichia/Shigella. Compared with EPA, DHA-supplemented mice have more abundant propionic/butyric acid-producing bacteria, including Coprococcus, Butyricimonas synergistica, Bacteroides acidifaciens, and Intestinimonas, and less-abundant LPS-correlated species Streptococcus and p-75-a5.

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“…Our data show that the gnotobiotic OMM 12 model is particularly well suited to study the molecular mechanisms involved in phage bacteria dynamics in the gut. OMM 12 community establishes a longterm stable composition over several mouse generations and can be used as platform to flexibly incorporate additional bacterial strains to the community , Herp et al 2019, Studer et al 2016. Interestingly, Hsu and colleagues recently reported the use of similar synthetic microbiota models to study phage bacteria interactions with two major differences.…”

Section: Discussionmentioning

confidence: 99%

The spatial heterogeneity of the gut limits bacteriophage predation leading to the coexistence of antagonist populations of bacteria and their viruses

Lourenço

Chaffringeon

Lamy-Besnier

et al. 2019

Preprint

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Bacteria and their viruses, bacteriophages (phages), are the most abundant components of the mammalian gut microbiota where these two entities coexist over time. The ecological dynamics underlying the coexistence between these two antagonistic populations in the gut are unknown. We challenged a murine synthetic bacterial community with a set of virulent phages, to study the factors allowing phages-bacteria coexistence in the gut. We found that coexistence was neither dependent on an arms race between bacteria and phages, nor on the ability of phages to extend host range.Instead, our data suggest that some phage-inaccessible sites in the mucosa of the ileum serve as a spatial refuge for bacteria, which from there disseminate in the gut lumen. Luminal phages amplify by infecting luminal bacteria maintaining phage throughout the gut. We conclude that the heterogeneous distribution of microbes in the gut contributes to the long-term coexistence of phages with phage-susceptible bacteria. This observation could explain the persistence in the human gut of intestinal phages, such as the crAssphage, as well as the low efficiency of oral phage therapy against enteric pathogens in animal models and clinical trials.

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Mucispirillum schaedleri Antagonizes Salmonella Virulence to Protect Mice against Colitis

Cited by 241 publications

References 84 publications

Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis

Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis

Eicosapentaenoic and Docosahexaenoic Acids Differentially Alter Gut Microbiome and Reverse High‐Fat Diet–Induced Insulin Resistance

The spatial heterogeneity of the gut limits bacteriophage predation leading to the coexistence of antagonist populations of bacteria and their viruses

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