Mucociliary transport determined by in vivo microdialysis in the airways of normal and CF mice

Abstract

We report a novel method to measure mucociliary transport (MCT) in both the upper and lower airways of normal and CF mice. The in vivo microdialysis technique involves placing a small quantity of dye on the airway surface and a microdialysis probe a defined distance from the site of dye deposition. The dye is transported toward the probe by ciliary transport and, upon reaching the microdialysis probe, diffuses across the dialysis membrane and is collected in the dialysate leaving the probe. The rate of MCT is calculated from the length of time from dye deposition to recovery. The rate of tracheal MCT in normal mice was 2.2 +/- 0.45 (SE) mm/min (n = 6), a value similar to that in reports using other techniques. MCT in CF mice was not different (2.3 +/- 0.29, n = 6), consistent with previous observations suggesting that tracheal ion transport properties are not different between CF and normal mice. The rate of MCT in the nasal cavity of normal mice was slower than in the trachea (1.3 +/- 0.26, n = 4). MCT in the CF mouse nasal cavity (1.4 +/- 0.31, n = 8), a region in which the CF mouse exhibits bioelectric properties similar to the human CF patient, was, again, not different from the normal mouse, perhaps reflecting copious gland secretion offsetting Na(+) and liquid hyperabsorption. In conclusion, we have developed a versatile, simple in vivo method to measure MCT in both upper and lower airways of mice and larger animals.

Normal nasal mucociliary clearance in CF children: evidence against a CFTR‐related defect

“…Interestingly, the authors also reported goblet cell meta-and hyperplasia, which they hypothesise was secondary to intermittent infection and inflammation caused by impaired clearance, although MCC was not measured. In a recent report, however, this group has reported normal MCC in both the upper and lower airways of CF mice [37]. ZAHM et al [38] reported slower tracheal MCC in CFTR tm1Unc CF mice, but also showed significant inflammation in the lamina propria.…”

Inhaled aerosol particle dosimetry in mice: A review

“…Particle clearance within the respiratory tract is dependent on the site of particle deposition. Particle clearance rates and mucociliary transport velocities in the upper respiratory tract (URT) and trachea have been reported for the mouse (Snipes et al, 1983;Brownstein, 1987;Hsieh et al, 1999;Foster et al, 2001;Grubb et al, 2004). Physiological factors in the mouse have been shown to be dependent on many factors, including strain, gender, age, and disease status.…”

“…Physiological factors in the mouse have been shown to be dependent on many factors, including strain, gender, age, and disease status. In addition, transgenic mice can be designed to alter specific physiological factors that might modify the inhaled, deposited, and retained doses of particles in the respiratory tract (Grubb et al, 2004;Mall, 2008). …”

Lung microdialysis—A powerful tool for the determination of exogenous and endogenous compounds in the lower respiratory tract (mini-review)

“…In animal models performing lung MD, usually CMA 10 8,12,13 or CMA 20 probes [12][13][14] (both CMA Microdialysis, Solna, Sweden) with membrane lengths of 10 to 16 mm were used. Some animal studies performed lung MD during cardiopulmonary bypass with cardiac arrest.…”

“…73 , 74 Recently, lung MD was employed for the determination of mucociliary transport in normal and cystic fi brosis (CF) mice. 13 An MD probe was implanted into the lumen of the trachea and a small quantity of dye was placed on the surface of a distal smaller bronchus at a known distance to the tip of the MD probe. The rate of bronchial mucociliary transport was calculated as the time from dye deposition to its fi rst detection in the MD samples.…”