Mucoepidermoid tumors of salivary glands: histoprognostic value of NORs stained with AgNOR technique

Chomette, G; Auriol, M; Labrousse, François; Vaillant, Jean

doi:10.1111/j.1600-0714.1991.tb00906.x

Cited by 31 publications

(12 citation statements)

References 4 publications

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“…PCNA immunostaining and AgNOR staining have been established as valuable methods with which the biological behavior of malignant cells can be examined [Robbins et al, 1987;Chomette et al, 1991;Tsuji et al, 1992;Cabrini et al, 1992]. PCNA was detected as a nuclear antigen reacting to autoantibodies in sera o f patients with systemic lupus eryth ematosus [Miyauchi et al, 1978], and is described as a non histone nuclear protein that appears in the nucleus during the late G1 phase, increases during the S phase and declines during G2 and M phases of the cell cycle [Celis and Celis, 1985].…”

Section: Discussionmentioning

confidence: 99%

Biological Behavior of Human Dental Pulp Cells in Response to Carious Stimuli Analyzed by PCNA Immunostaining and AgNOR Staining

Kobayashi

Izumi²,

Okamura³

et al. 1996

Caries Res

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The change in proliferative and metabolic activities of human dental pulp cells responding to carious stimuli was studied by means of immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and silver-binding nucleolar organizer region (AgNOR) staining. We classified the pulp tissues into five groups according to the progression of dental caries, ranging from grade 0 (the pulp of noncarious teeth) to grade 4 (the pulp of perforated carious teeth). PCNA-positive pulp cells were detected only in advanced dental caries (grades 3 and 4), and the difference in immuno-positive rate was significant between the two grades (p < 0.001). However, the mean number of AgNORs per nucleus increased even in the early phase of dental caries, significant differences being detected between grades 1 and 2 (p < 0.005), 2 and 3 (p < 0.005), and 3 and 4 (p < 0.001). Our data suggested that the metabolic activity of dental pulp cells was enhanced in the early phase of dental caries. However, proliferation of pulp cells occurred later in small degrees during fully developed caries such as grades 3 and 4. The slow and weak response in cellular proliferation might contribute to the usual fragility of the pulp to various assaults including caries or pulpitis.

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Section: Discussionmentioning

confidence: 99%

Biological Behavior of Human Dental Pulp Cells in Response to Carious Stimuli Analyzed by PCNA Immunostaining and AgNOR Staining

Kobayashi

Izumi²,

Okamura³

et al. 1996

Caries Res

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“…Due to its simplicity and sensitivity, its rapid and convenient nature (6)(7)(8)(9)(10)(11), and its applicability to paraffin sections, argyrophillic nuclear organizer region (AgNOR) analysis was used in the present study. Nucleolar organizer regions (NORs) are loops of DNA that encode ribosomal RNA and are considered to be important in the synthesis of proteins.…”

Section: Introductionmentioning

confidence: 99%

“…The grading system for MEC reported by Seifert et al, which is based on proportion of cells in a given section, is less reliable than AgNOR count in MEC in the context of MEC outcome (6). Auclair et al described a threetiered grading system based on various histopathological features ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

Correlation between mucoepidermoid carcinoma grade and AgNOR count

et al. 2010

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Mucoepidermoid carcinoma (MEC) is a malignant glandular epithelial neoplasm having an unpredictable behavior and a tendency to recur. Numerous parameters have been assessed to predict the outcome of this lesion, but have been deemed inadequate, with the exception of tumor stage and grade. In the present study, we attempted to correlate the proliferative activity of MEC with its histopathological grade, using argyrophillic nuclear organizer region (AgNOR) count. Thirty cases of MEC were included in the study. All the slides were stained using hematoxylin and eosin and silver nitrate techniques. Counting was performed at a magnification of x1,000 with an oil-immersion lens. Positive correlations were seen between AgNOR count and MEC grade (P < 0.05), with AgNOR count increasing in proportion with tumor grade. The AgNOR count in various grades of MEC indicates a relative progression in the proliferative activity of this tumor. This index is positively correlated with tumor grade, although there are some exceptions. The utility of AgNOR count in predicting the prognosis of MEC can be considered of importance; however, further assessment, such as survival studies, is necessary.

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“…Thus, this method has become an important auxiliary tool for the anatomo‐pathological diagnosis (6, 12, 13). Likewise, it has been used to diagnose oral diseases, such as salivary glands tumors (4, 14, 15), odontogenic cysts, odontogenic fibroma, ameloblastoma (16) and different lesions that affect the oral mucus, generally of cancerous development (17–21).…”

mentioning

confidence: 99%

Nucleolar organizer regions (NORs) evaluation of lingual salivary glands of chronic alcoholics

Severgnini

Ferraris

Carranza

2002

J Oral Pathology Medicine

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Mucoepidermoid tumors of salivary glands: histoprognostic value of NORs stained with AgNOR technique

Cited by 31 publications

References 4 publications

Biological Behavior of Human Dental Pulp Cells in Response to Carious Stimuli Analyzed by PCNA Immunostaining and AgNOR Staining

Biological Behavior of Human Dental Pulp Cells in Response to Carious Stimuli Analyzed by PCNA Immunostaining and AgNOR Staining

Correlation between mucoepidermoid carcinoma grade and AgNOR count

Nucleolar organizer regions (NORs) evaluation of lingual salivary glands of chronic alcoholics

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