Mucoprotective effects of Saikosaponin-A in 5-fluorouracil-induced intestinal mucositis in mice model

Ali, Jawad; Khan, Ashraf Ullah; Shah, Fawad Ali; Ali, Hussain; Islam, Salman Ul; Kim, Yeong Shik; Khan, Salman

doi:10.1016/j.lfs.2019.116888

Cited by 73 publications

(59 citation statements)

References 42 publications

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“…In short, the effect of the poncirin treatment on the GST, GSH, Catalase and SOD was evaluated against the CCL4-induced liver injury. For the determination of the GST, 0.1 ml of the liver homogenate was mixed with the equal volume of 1-chloro-2, 4-dinitrobenzene (CDNB) and the final volume was adjusted to 3 ml by the addition of the phosphate buffer (PH: 6.0) [34]. The absorbance was recorded at 314 nm.…”

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

“…The absorbance was recorded at 314 nm. The GSH concentration was determined by mixing of 0.1 ml liver homogenate with the 0.5 ml of the 5,5dithio-bis-2-nitrobenzoic acid (DTNB) and the final volume was made 3 ml by the addition of the phosphate buffer, while the absorbance was recorded at 412 nm [34]. The Catalase concentration was assessed in liver homogenate by adding 0.1 ml of the tissue homogenate with the 2.9 ml of H 2 O 2 buffer and the absorbance was recorded at 240 nm.…”

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

“…The Catalase concentration was assessed in liver homogenate by adding 0.1 ml of the tissue homogenate with the 2.9 ml of H 2 O 2 buffer and the absorbance was recorded at 240 nm. The SOD concentration was determined in the liver homogenate by the mixing the 0.2 ml of the sample with the 2.6 ml of Tris-EDTA and 0.1 ml of the pyragallol, while the absorbance was noted at 420 nm [34]. However, the lipid peroxidation product i.e.…”

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

“…MDA was determined by incubation of the 0.25 ml tissue homogenate at 37°C for 1 h in water bath. After this, 5% of the trichloroacetic acid (TCA) and 0.5 ml (0.67%) thiobarbituric acid (TBA) was added to the tissue homogenate and the reading was noted at 535 nm [34].…”

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

“…The permeability of Evans blue into the tissue such as peritoneum and liver were assessed in all the treated groups according to the reported methods with little modifications [34]. The vascular permeability was assessed by administering the Evans blue dye 30 min after the poncirin administration into the tail vein.…”

Section: Evans Blue Vascular Permeabilitymentioning

confidence: 99%

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Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice

Ullah

Khan

Baig

et al. 2020

BMC Complement Med Ther

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Background: In the present study, the poncirin which is flavonoid-7-o-glycosides (isolated from the Poncirus trifoliata) in nature was evaluated against the Carbon tetra chloride (CCL4)-induced liver injury. The poncirin have been reported for various anti-inflammatory, analgesic activity etc. Based on the previous studies it was anticipated that the poncirin will ameliorate CCL4-induced liver injury.Methods: The CCL4-induced acute and chronic liver injury model (albino BALB/c mice) was used. Following the induction of the liver injury various parameters such as food and water intake, body weight and weight to dry ratio changes were assessed. Furthermore, various hematological, biochemical parameters and histological studies such as hemotoxylin and eosin (H and E) staining were performed. The poncirin treatment was also evaluated against the pro-inflammatory cytokines such as interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) using enzyme link immunosorbant assay (ELISA). The Swiss Target prediction software was used to investigate interaction of the poncirin on the various hepatic enzymes. Results: The poncirin treatment markedly improved the behavioral parameters such as food and water intake. The liver weight variation was attenuated and total body was improved markedly. The hematological and biochemical parameters were significantly improved compared to the CCL4 treated groups. The anti-oxidants were induced, while oxidative stress markers were reduced promisingly. The H and E staining showed that poncirin treatment significantly improved the histology of liver compared to the CCL4 treated group. Furthermore, the poncirin treatment also evidently decreased the inflammatory mediators. Conclusions: The poncirin treatment showed marked improvement in behavioral, biochemical and histological parameters following CCL4-induced liver injury. Additionally, the poncirin treatment also markedly improved the antioxidant enzymes, attenuated the oxidative stress markers and inflammatory cytokines.

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Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

Section: Effect Of Poncirin On Liver Anti-oxidants and Oxidative Strementioning

confidence: 99%

Section: Evans Blue Vascular Permeabilitymentioning

confidence: 99%

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Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice

Ullah

Khan

Baig

et al. 2020

BMC Complement Med Ther

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Thymoquinone protects against 5‐Fluorouracil‐induced mucositis by NF‐κβ and HIF‐1 mechanisms in mice

Madani

Kazemi

Shirafkan

et al. 2023

J Biochem & Molecular Tox

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Mucositis is among the most common side effects of 5‐Fluorouracil (5‐FU) and other cancer therapeutic drugs. Thymoquinone (TQ), a bioactive constituent extracted from Nigella sativa, has antioxidant and anti‐inflammatory properties and can modify acute gastrointestinal injury. To investigate the effects of TQ on mucositis induced by 5‐FU, studied animals were divided into four groups: control, 5‐FU unit dose (300 mg/kg) to cause oral and intestinal mucositis (OM and IM), TQ (2.5 mg/kg) and TQ (2.5 mg/kg) plus 5‐FU. Due to The molecular mechanisms, it was confirmed that the expression of NF‐κβ and HIF‐1 increases in OM. The serum levels of malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD), as well as pathological parameters, were assessed. Based on our results, the nuclear factor‐kappa β gene expression in the tongue was downregulated significantly in the 5‐FU + TQ compared to the 5‐FU. TQ treatment can diminish MDA, and a reduction in oxidative stress was shown. TQ could also reduce the severity of tissue destruction and damaging effects induced by 5‐FU on the tongue and intestine. We also observed lower villus length and width in the intestine of the 5‐FU group compared to the control group. According to our research's pathological, biochemical, and molecular results, treatment with TQ as an anti‐inflammatory and antioxidant compound may be the potential to improve and treat 5‐FU‐induced OM and IM, and TQ could be used against cancer treatment drugs and exhibit fewer adverse effects.

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RETRACTED ARTICLE: Inhibition of NF-κB signaling and HSP70/HSP90 proteins by newly synthesized hydrazide derivatives in arthritis model

Khan

et al. 2021

Naunyn-Schmiedeberg's Arch Pharmacol

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Mucoprotective effects of Saikosaponin-A in 5-fluorouracil-induced intestinal mucositis in mice model

Cited by 73 publications

References 42 publications

Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice

Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice

Thymoquinone protects against 5‐Fluorouracil‐induced mucositis by NF‐κβ and HIF‐1 mechanisms in mice

RETRACTED ARTICLE: Inhibition of NF-κB signaling and HSP70/HSP90 proteins by newly synthesized hydrazide derivatives in arthritis model

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