Mucosal Delivery of a Self-destructing Salmonella-Based Vaccine Inducing Immunity Against Eimeria

Wang, Xiao; Fields, Emilia; Okon, Blessing; Jenkins, Mark C.; Wilkins, G. C.; Brovold, Matthew; Golding, Tiana; Gonzales, Amanda; Golden, Greg; Clark‐Curtiss, Josephine E.; Curtiss, Roy

doi:10.1637/aviandiseases-d-19-00159

Cited by 8 publications

(9 citation statements)

References 83 publications

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“…Strain χ12341 also has an additional Δ sifA mutation, which enables Salmonella to escape the endosome (termed the Salmonella containing vesicle, SCV ) for lysis in the cytosol ( Beuzón et al, 2000 ). Previous work demonstrated that a vaccine vector strain with the Δ sifA mutation conferred higher levels of protection when compared with a sifA + strain against influenza virus and Eimeria challenges ( Ashraf et al, 2011 ; Kong et al, 2020 ). These collective changes in χ12341 compared to χ11802 should enhance the attenuation, efficiency of lysis and biocontainment, and immunogenicity against clostridial antigens.…”

Section: Resultsmentioning

confidence: 99%

“…( Branger et al, 2009 ), Streptococcus pneumoniae ( Nayak et al, 1998 ) and Eimeria spp. ( Kong et al, 2020 ) also showed PIESVs with an empty vector giving greater levels of protection compared to administering buffer saline. Salmonella has multiple ways to activate innate immune responses through pathogen associated molecular patterns ( PAMPs ) and damage associated molecular patterns ( DAMPs ), so that these strains can act as potent adjuvants.…”

Section: Discussionmentioning

confidence: 99%

“…Although direct verification of the derepression time is impossible since both strains require arabinose to survive, the repressed levels of PlcC and NetB in χ12341 were less than in χ11802, corresponding to reduced levels of LacI in χ12341 ( Figure 2 ), indicating that this could be the expected scenario in vivo. The second mutation is the Δ sifA mutation based on previous evidence demonstrated in influenza challenge ( Ashraf et al, 2011 ; Kong et al, 2012 ) and in other studies on inducing protective immunity against Eimeria challenge ( Kong et al, 2020 ). The release of antigen due to programmed lysis within the SCV would likely stimulate an MHC II antigen presentation, dendritic cell migration, and adaptive immune responses ( Mitchell et al, 2004 ; Halici et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

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A triple-sugar regulated Salmonella vaccine protects against Clostridium perfringens-induced necrotic enteritis in broiler chickens

et al. 2022

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Gram-positive Clostridium perfringens type G, the causative agent of necrotic enteritis ( NE ), has gained more attention in the poultry industry due to governmental restrictions on the use of growth-promoting antibiotics in poultry feed. Our previous work has proved that regulated delayed lysis Salmonella vaccines delivering a plasmid encoding an operon fusion of the nontoxic C-terminal adhesive part of alpha toxin and a GST-NetB toxin fusion were able to elicit significant protective immunity in broilers against C. perfringens challenge. We recently improved our S. Typhimurium antigen delivery vaccine strain by integrating a rhamnose-regulated O-antigen synthesis gene enabling a triple-sugar regulation system to control virulence, antigen-synthesis and lysis in vivo traits. The strain also includes a Δ sifA mutation that was previously shown to increase the immunogenicity of and level of protective immunity induced by Salmonella vectored influenza and Eimeria antigens. The new antigen-delivery vaccine vector system confers on the vaccine strain a safe profile and improved protection against C. perfringens challenge. The strain with the triple-sugar regulation system delivering a regulated lysis plasmid pG8R220 encoding the PlcC and GST-NetB antigens protected chickens at a similar level observed in antibiotic-treated chickens. Feed conversion and growth performance were also similar to antibiotic-treated chickens. These studies made use of a severe C. perfringens challenge with lesion formation and mortality enhanced by pre-exposure to Eimeria maxima oocysts. The vaccine achieved effectiveness through three different immunization routes, oral, spray and in drinking water. The vaccine has a potential for application in commercial hatcher and broiler-rearing conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A triple-sugar regulated Salmonella vaccine protects against Clostridium perfringens-induced necrotic enteritis in broiler chickens

et al. 2022

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“…The advantage of using live bacterial vaccine vectors is their capability to invade and colonize internal tissues after mucosal delivery, which is critically important for inducing immune protection (32). Bacterial vaccine vectors have been used to produce protection against self as well as heterologous antigens (42)(43)(44). Our lab has developed a strategy of regulated-delayed attenuation in Salmonella, which makes the vaccine vectors safe and immunogenic for delivery of heterologous antigens to mice (45)(46)(47).…”

Section: Discussionmentioning

confidence: 99%

Construction and Evaluation of Recombinant Attenuated Edwardsiella piscicida Vaccine (RAEV) Vector System Encoding Ichthyophthirius multifiliis (Ich) Antigen IAG52B

2022

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We have successfully designed and constructed a RAEV vector system with regulated-delayed attenuation in vivo attributes that synthesizes Ichthyophthirius multifiliis (Ich) protective antigen IAG52B to enable vaccination of fish susceptible to edwardsiellosis and white spot disease. The first feature of this vaccine delivery system is an Edwardsiella piscicida strain carrying genomic deletions of asdA. AsdA is an enzyme necessary for the synthesis of diaminopimelic acid (DAP), which is an essential component of the peptidoglycan layer of the cell wall of Gram-negative bacteria. asdA mutant strains have obligate growth requirements for DAP in the medium or a plasmid vector with the wild-type asdA gene enabling synthesis of DAP. This balanced-lethal plasmid vector-host system in E. piscicida enables as a second feature the synthesis of recombinant antigens to induce protective immunity against fish pathogens. Recombinant protective antigen IAG52B from the fish pathogen I. multifiliis was synthesized by RAEV strains harboring the AsdA+ plasmid pG8R8029. The third feature of this vaccine strain is a regulated-delayed attenuation in vivo phenotype that is based on the replacement of an arabinose-regulated araC ParaBAD cassette for the promoters of the fur and crp genes of E. piscicida such that the expression of these genes is dependent on arabinose provided during growth. Thus, following colonization, the Fur and Crp proteins stop being synthesized due to the lack of arabinose and attenuation is progressively achieved in vivo to prevent generation of diseases symptoms. Our vaccine strain χ16022 with the genotype ΔasdA10 ΔPfur170::TT araC ParaBADfur ΔPcrp68::TT araC ParaBADcrp contains the AsdA+ plasmid, pG8R8029, which encodes the IAG52B antigen. Vaccine strain χ16022(pG8R8029) is attenuated and induces systemic and mucosal IgM titer against E. piscicida and Ich in zebrafish. In addition, transcript levels of tnf-α, il-1β, il-6 and il-8 were significantly increased in different tissues of vaccinated zebrafish compared to unimmunized fish. Zebrafish vaccinated with χ16022(pG8R8029) showed 60% survival upon intracoelomic (i.c.) challenge with a lethal dose of virulent E. piscicida strain J118. Our RAEV system could be used as a generalized vaccine-vector system to protect teleost fish against multiple bacterial, viral and parasitic infectious diseases.

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“…The delivery system of recombinant or subunit vaccine candidates, using eukaryote or prokaryote vectors for feed or water administration application have shown optimistic and realistic results [ 36 , 37 , 38 , 39 , 40 ]. Some of these novel vaccines not only provide antigenic diversity, but cost of production and welfare issues are significantly reduced or eliminated [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Juárez-Estrada

Gayosso-Vázquez

Téllez-Isaías

et al. 2021

Animals

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This study investigated protection against Eimeria tenella following the vaccination of chicks with 5.3 × 106 E. tenella whole-sporozoites emulsified in the nanoparticle adjuvant IMS 1313 N VG Montanide™ (EtSz-IMS1313). One-day-old specific pathogen-free (SPF) chicks were subcutaneously injected in the neck with EtSz-IMS1313 on the 1st and 10th days of age. Acquired immunity was assayed through a challenge with 3 × 104 homologous sporulated oocysts at 21 days of age. The anticoccidial index (ACI) calculated for every group showed the effectiveness of EtSz-IMS1313 as a vaccine with an ACI of 186; the mock-injected control showed an ACI of 18 and the unimmunized, challenged control showed an ACI of −28. In a comparison assay, antibodies from rabbits and SPF birds immunized with EtSz-IMS1313 recognized almost the same polypeptides in the blotting of E. tenella sporozoites and merozoites. However, rabbit antisera showed the clearest recognition pattern. Polypeptides of 120, 105, 94, 70, 38, and 19 kDa from both E. tenella life cycle stages were the most strongly recognized by both animal species. The E. tenella zoite-specific IgG antibodies from the rabbits demonstrated the feasibility for successful B cell antigen identification.

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Mucosal Delivery of a Self-destructing Salmonella-Based Vaccine Inducing Immunity Against Eimeria

Cited by 8 publications

References 83 publications

A triple-sugar regulated Salmonella vaccine protects against Clostridium perfringens-induced necrotic enteritis in broiler chickens

A triple-sugar regulated Salmonella vaccine protects against Clostridium perfringens-induced necrotic enteritis in broiler chickens

Construction and Evaluation of Recombinant Attenuated Edwardsiella piscicida Vaccine (RAEV) Vector System Encoding Ichthyophthirius multifiliis (Ich) Antigen IAG52B

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

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