Mucosal delivery of herpes simplex virus vaccine

Bowen, J.C.; Alpar, H. Oya; Phillpotts, R. J.; Brown, M. R. W.

doi:10.1016/s0923-2516(06)80115-9

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…Extrapolation from extensive rodent studies suggests that the pre-erythrocytic immunity to P. falciparum infection is mediated, at least in part, by major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTLs) acting against the intrahepatic forms (20,21). In addition, LS antigen 1 (LSA1)-specific HLA class I-restricted CD8 ϩ -CTL responses correlate with resistance to severe malaria in humans (16). Based on these observations, one logical hypothesis is that an effective pre-erythrocytic malaria vaccine is likely to require the inclusion of intrahepatic T-cell epitopes capable of inducing CTL activity (20,34,43).…”

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confidence: 99%

Long-Term Multiepitopic Cytotoxic-T-Lymphocyte Responses Induced in Chimpanzees by Combinations ofPlasmodium falciparumLiver-Stage Peptides and Lipopeptides

2004

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Preclinical immunogenicity studies of 12 malaria peptides, selected from four Plasmodium falciparum antigens (Ags), namely, LSA1, LSA3, SALSA, and STARP, that are expressed at the pre-erythrocytic (sporozoite and liver) stages of the human parasite were carried out in chimpanzees. To strengthen their immunogenicity, six of these synthetic peptides were modified by the C-terminal addition of a single palmitoyl chain (lipopeptides) and delivered without adjuvant, whereas the remaining six unmodified peptides were emulsified and delivered by using Montanide ISA51 adjuvant. We have previously reported that these peptides and lipopeptides induce high B-cell and CD4؉ -T-helper responses in chimpanzees. In this report, we show their ability to induce multiepitopic and long-lasting antigen-specific CD8؉ cytotoxic-T-lymphocyte (CTL) responses. The magnitude, consistency, and memory of CTL responses generated by LSA3 peptides point to the strong immunogenicity of this liver-stage Ag. These findings support the screening strategy used to select the four P. falciparum pre-erythrocytic Ags and emphasize their valuable immunogenic properties. The successful immunization of nonhuman primates with combinations of corresponding peptides in a mineral oil emulsion (ISA51) and lipopeptides in saline provide a vaccine formulation that can be tested in humans.

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confidence: 99%

Long-Term Multiepitopic Cytotoxic-T-Lymphocyte Responses Induced in Chimpanzees by Combinations ofPlasmodium falciparumLiver-Stage Peptides and Lipopeptides

2004

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“…route failed to induce as effective immune responses (data not shown) and protection against local, clinical HSV-2 disease or virus replication, as could be achieved following subcutaneous inoculation with the same preparation at an equivalent dosage. Using a subunit glycoprotein HSV-l vaccine, other workers have reported similar results (Bowen et al, 1992). In addition, administration oflive HSV -1 DISC vaccine to guineapigs by the i.n.…”

Section: Discussionmentioning

confidence: 58%

Vaccine Design

1997

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Aseries presenting the results of aetlvities sponsored by the NA TO Seienee Committee, whieh aims at the dissemination of advaneed seientlflc and technologleal knowledge, with a vlew to strengthenlng links between seientific communities. The series is publlshed by an International board of publlshers in conjunctlon with the NATO Sclentific Affairs Division A Llfe Sclences B Physlcs All rights reservedNo part 01 thls book may be reproduced, stored in a retrleval syst~m, or transmitted in any lorm or by any means, electronlc, mechanlcal, photocopylng, mlcrolilming. recording, or otherwlse, wlthout written perm Iss Ion lrom the Publlsher ISBN 978-1-4899-0064-7 ISBN 978-1-4899-0062-3 (eBook) PREFACEDuring the last decade or so vaccine development has been facilitated by rapid advances in the molecular and cell biology ofthe immune system. This has laid the foundations of a new generation of vaccines exemplified by subunit vaccines produced through gene cloning and by synthetic peptides mimicking small regions ofproteins on the outer coat ofviruses. However, as subunit and peptide vaccines are only weakly or non-immunogenic, there is areal need for strategies to improve their potency.This book contains the proceedings of the 5th NATO Advanced Studies Institute (ASI), "Vaccine Design: The Role of Cytokine Networks," held at Cape Sounion Beach, Greece, during 24 June-5 July 1996 and deals in depth with the role ofbasic immunology in the regulation of immunity and vaccine design. Special emphasis is given to the use of cytokines in conjuction with vaccines with the aim ofimproving their potency or the use ofvaccines designed to improve cytokine production.

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“…The potential of mucosal immunization with live attenuated/modified HSV-2 for prevention of latency in the guinea pig model of the disease has previously been reported (34–38). Furthermore, nasal immunization with recombinant HSV-2 gD together with the adjuvant LTK63 was shown to generate partial protection against primary genital herpes in guinea pigs (39).…”

Section: Discussionmentioning

confidence: 98%

Nasal Immunization Confers High Avidity Neutralizing Antibody Response and Immunity to Primary and Recurrent Genital Herpes in Guinea Pigs

et al. 2016

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Genital herpes is one of the most prevalent sexually transmitted infections in both the developing and developed world. Following infection, individuals experience life-long latency associated with sporadic ulcerative outbreaks. Despite many efforts, no vaccine has yet been licensed for human use. Herein, we demonstrated that nasal immunization with an adjuvanted HSV-2 gD envelope protein mounts significant protection to primary infection as well as the establishment of latency and recurrent genital herpes in guinea pigs. Nasal immunization was shown to elicit specific T cell proliferative and IFN-γ responses as well as systemic and vaginal gD-specific IgG antibody (Ab) responses. Furthermore, systemic IgG Abs displayed potent HSV-2 neutralizing properties and high avidity. By employing a competitive surface plasmon resonance (SPR) analysis combined with a battery of known gD-specific neutralizing monoclonal Abs (MAbs), we showed that nasal immunization generated IgG Abs directed to two major discontinuous neutralizing epitopes of gD. These results highlight the potential of nasal immunization with an adjuvanted HSV-2 envelope protein for induction of protective immunity to primary and recurrent genital herpes.

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Mucosal delivery of herpes simplex virus vaccine

Cited by 12 publications

References 22 publications

Long-Term Multiepitopic Cytotoxic-T-Lymphocyte Responses Induced in Chimpanzees by Combinations ofPlasmodium falciparumLiver-Stage Peptides and Lipopeptides

Long-Term Multiepitopic Cytotoxic-T-Lymphocyte Responses Induced in Chimpanzees by Combinations ofPlasmodium falciparumLiver-Stage Peptides and Lipopeptides

Vaccine Design

Nasal Immunization Confers High Avidity Neutralizing Antibody Response and Immunity to Primary and Recurrent Genital Herpes in Guinea Pigs

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