Mucosal immune responses and protective efficacy elicited by oral administration AMP-ZnONPs-adjuvanted inactivated H9N2 virus in chickens

Moringa oleifera (M. oleifera) is a plant with significant medicinal and nutritional value and contains various bioactive compounds, particularly in its leaves (MOL). This study sought to explore the impact of M. oleifera leaf polysaccharides (MOLPs) on lipopolysaccharide (LPS)-activated intestinal epithelial cells (IEC6) and to uncover the mechanisms involved. The cytotoxicity of MOLP on IEC6 cells was assessed using the Cell Counting Kit-8 (CCK-8) assay, which demonstrated a safe concentration range of 0–1280 µg/mL. The impact of MOLP on cell viability was further evaluated over 12 to 48 h. IEC6 cells were treated with three concentrations of MOLP low (25 µg/mL), medium (50 µg/mL), and high (100 µg/mL) alongside LPS (50 µg/mL) stimulation for one day. The findings revealed that treatment with MOLP significantly promoted cell migration and increased the production of interleukin-10 (IL-10), while it simultaneously decreased cell apoptosis and the levels of pro-inflammatory cytokines, such as tumour necrosis factor alpha (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6). Additionally, MOLP treatments across all concentrations significantly reduced the expression of Toll-like receptor 4 (TLR-4), myeloid differentiation primary response 88 (MyD88), phosphorylated nuclear factor kappa B-alpha (pIκB-α), and phosphorylated NF-κB p65 signalling pathways. Moreover, MOLP restored the expression of tight junction proteins, such as zonula occludens-1 (ZO-1) and occludin, which had been disrupted by LPS. These results indicate that MOLP exhibits anti-inflammatory properties by inhibiting inflammatory signalling pathways and maintaining intestinal barrier integrity through the upregulation of tight junction proteins in IEC6 cells. This study enhances our understanding of the anti-inflammatory capabilities of MOLP.

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Effects of Whole Flaxseed in Reduced Energy Diets on Broiler Chickens Meat Quality and Carcass Characteristics Under Heat Stress Condition

Harith M. Jawad,

Yasser J. Jameel

2024

Aca. Intl. J. V. Med.

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This study aimed to assess the impact of heat stress (HS) on broiler chickens provided with a low-energy diet including whole flaxseed, focusing on carcass characteristics and meat quality. A total of 250 one-day-old Ross 308 broiler chicks were randomly allocated into five groups (50 chicks per group), with two replicates for each group. The negative control treatment (T1) received a basal diet devoid of HS. The second treatment fed a basal diet under HS as a positive control (T2). In the third experiment, they fed a basal diet, including whole flaxseed, under heat-stress conditions (T3). The fourth treatment consisted of providing a low-energy diet with whole flaxseed under HS (T4). The fifth intervention was a reduced energy diet administered under HS (T5). The carcass characteristics and edible entrails in the T3 and T4 groups showed a significant improvement (P≤0.05) compared to the control groups. The meat quality in the T3 and T4 groups was considerably enhanced (P≤0.05) due to an increase in omega-3 fatty acid levels [α-linolenic acid, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA)], as determined by GC mass spectrometry compared to the control group that had a corn-soybean diet. In conclusion, incorporating whole flaxseeds into a low-energy diet during HS augmented broiler chickens’ productivity, improved carcass characteristics, and elevated omega-3 concentrations in the meat.

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Mucosal immune responses and protective efficacy elicited by oral administration AMP-ZnONPs-adjuvanted inactivated H9N2 virus in chickens

Cited by 3 publications

References 35 publications

Peptides as Alternatives to Antibiotics in Poultry Health Management

Peptides as Alternatives to Antibiotics in Poultry Health Management

The Anti-Inflammatory Properties of Polysaccharides Extracted from Moringa oleifera Leaves on IEC6 Cells Stimulated with Lipopolysaccharide In Vitro

Effects of Whole Flaxseed in Reduced Energy Diets on Broiler Chickens Meat Quality and Carcass Characteristics Under Heat Stress Condition

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