Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Verdonck, Frank; Tiels, Petra; Gog, K van; Goddeeris, Bruno; Lycke, Nils; Clements, John D.; Cox, Eric

doi:10.1016/j.vetimm.2007.06.018

Cited by 39 publications

(32 citation statements)

References 57 publications

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“…The control of ED has mainly been based on adjusting diet components, bacterial probiotics, and antibiotic therapy (Bertschinger 1999;Uemura et al 2003). Several attempts to stimulate immune protection against ED have been made (MacLeod and Gyles 1991;Bosworth et al 1996;Verdonck et al 2007). However, to date, there is no commercial vaccine available or an effective therapy.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

Oanh

Nguyen

Thuy

et al. 2010

Trop Anim Health Prod

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Edema disease (ED) is a common fatal disease in newly weaned piglets. To develop an effective control program for ED, we carried out a study to better understand the incidence and spread of the disease and the characteristics of the causative agent. In our study, 69 Escherichia coli strains, isolated from 92 piglets showing clinical signs of ED from 13 provinces in northern Vietnam, were positive for both the VT2e toxin and the F18 major fimbrial subunit gene fedA. Of these, 40 strains (58%) were positive for AIDA and 16 isolates carried one or more enterotoxins. Forty-six (67%) of the 69 VT2e(+)/F18(+) E. coli isolates belonged to classical serotypes (O139:K82, O141: K85, O138:K81, and O149:K91) while the remaining strains did not belong to the common serotypes in pig. Seropathotype 0139:K82(+)/VT2e(+)/F18(+)/AIDA(+) (21 isolates) was the most frequently detected ED-causing E. coli strain. High prevalence of resistance was observed to the common drugs of tetracycline, streptomycin, trimethoprim/sulfamethoxazole, amoxicillin/clavulanic acid, and spectinomycin. Multiple resistances were widely distributed with 84% of isolates resistant to five antibiotics. Sequence analysis demonstrated that the VT2e toxin is identical among E. coli strains causing ED in pig.

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Section: Introductionmentioning

confidence: 99%

Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

Oanh

Nguyen

Thuy

et al. 2010

Trop Anim Health Prod

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“…Postweaning pigs remain immunologically naïve to ETEC, and they develop diarrhea after ETEC infection. Immunization of weaned pigs with vaccines containing K88 and/or F18 fimbrial antigens induces anti-K88 and/or anti-F18 antibodies (32,33,35). However, these products are not likely to fully protect weaned pigs against postweaning diarrhea (10).…”

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confidence: 99%

A Tripartite Fusion, FaeG-FedF-LT₁₉₂A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Ruan¹,

Liu²,

Casey³

et al. 2011

Clin. Vaccine Immunol.

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Enterotoxigenic Escherichia coli (ETEC) strains expressing K88 (F4) or F18 fimbriae and heat-labile (LT)and/or heat-stable (ST) toxins are the major cause of diarrhea in young pigs. Effective vaccines inducing antiadhesin (anti-K88 and anti-F18) and antitoxin (anti-LT and anti-ST) immunity would provide broad protection to young pigs against ETEC. In this study, we genetically fused nucleotides coding for peptides from K88ac major subunit FaeG, F18 minor subunit FedF, and LT toxoid (LT 192 ) A2 and B subunits for a tripartite adhesin-adhesin-toxoid fusion (FaeG-FedF-LT 192 A2:B). This fusion was used for immunizations in mice and pigs to assess the induction of antiadhesin and antitoxin antibodies. In addition, protection by the elicited antiadhesin and antitoxin antibodies against a porcine ETEC strain was evaluated in a gnotobiotic piglet challenge model. The data showed that this FaeG-FedF-LT 192 A2:B fusion elicited anti-K88, anti-F18, and anti-LT antibodies in immunized mice and pigs. In addition, the anti-porcine antibodies elicited neutralized cholera toxin and inhibited adherence against both K88 and F18 fimbriae. Moreover, immunized piglets were protected when challenged with ETEC strain 30302 (K88ac/LT/STb) and did not develop clinical disease. In contrast, all control nonvaccinated piglets developed severe diarrhea and dehydration after being challenged with the same ETEC strain. This study clearly demonstrated that this FaeG-FedF-LT 192 A2:B fusion antigen elicited antibodies that neutralized LT toxin and inhibited the adherence of K88 and F18 fimbrial E. coli strains and that this fusion could serve as an antigen for vaccines against porcine ETEC diarrhea. In addition, the adhesin-toxoid fusion approach used in this study may provide important information for developing effective vaccines against human ETEC diarrhea.

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“…However, oral immunization with F18 fimbriae does not elicit protection in a piglet model, probably due to the presence of immunodominant epitopes in the FedA subunit. 28 We reasoned that oral immunization of piglets with purified FedF subunit would enable induction of protective immunity, but only if FedF is encapsulated inside microparticles so asto deliver higher amounts of antigens to the gut-associated lymphoid tissue and to induce strong immune responses by mimicking pathogen dimensions.…”

Section: Introductionmentioning

confidence: 99%

Duality of β-glucan microparticles: antigen carrier and immunostimulants

Baert¹,

Geest²,

Greve³

et al. 2016

IJN

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Designing efficient recombinant mucosal vaccines against enteric diseases is still a major challenge. Mucosal delivery of recombinant vaccines requires encapsulation in potent immunostimulatory particles to induce an efficient immune response. This paper evaluates the capacity of β-glucan microparticles (GPs) as antigen vehicles and characterizes their immune-stimulatory effects. The relevant infectious antigen FedF was chosen to be loaded inside the microparticles. The incorporation of FedF inside the particles was highly efficient (roughly 85%) and occurred without antigen degradation. In addition, these GPs have immunostimulatory effects as well, demonstrated by the strong reactive oxygen species (ROS) production by porcine neutrophils upon their recognition. Although antigen-loaded GPs still induce ROS production, antigen loading decreases this production by neutrophils for reasons yet unknown. However, these antigen-loaded GPs are still able to bind their specific β-glucan receptor, demonstrated by blocking complement receptor 3, which is the major β-glucan receptor on porcine neutrophils. The dual character of these particles is confirmed by a T-cell proliferation assay. FedF-loaded particles induce a significantly higher FedF-specific T-cell proliferation than soluble FedF. Taken together, these results show that GPs are efficient antigen carriers with immune-stimulatory properties.

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Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Cited by 39 publications

References 57 publications

Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

A Tripartite Fusion, FaeG-FedF-LT₁₉₂A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Duality of β-glucan microparticles: antigen carrier and immunostimulants

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Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Cited by 39 publications

References 57 publications

Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

A Tripartite Fusion, FaeG-FedF-LT192A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Duality of &beta;-glucan microparticles: antigen carrier and immunostimulants

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Resources

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A Tripartite Fusion, FaeG-FedF-LT₁₉₂A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Duality of β-glucan microparticles: antigen carrier and immunostimulants