Mucous cells of the tracheobronchial tree in the ferret

Jacob, Samuel; Poddar, S.

doi:10.1007/bf00493372

Cited by 23 publications

(12 citation statements)

References 13 publications

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“…In accordance with many authors (Lamb and Reid 1972;Jones and Reid 1978;Jacob and Poddar 1982;Spicer et al 1983;Castells et al 1990Castells et al , 1991Castells et al , 1992Castells et al , 1994 Mandal and Mandal 1990;Jeffery et al 1992), we found both neutral and acidic sulphated and acidic sialylated glycoconjugates in the mucous granules of goblet cells in the tracheal epithelium of the control rabbits. In experimental groups, we identified the same glycoconjugates, too.…”

Section: Discussionsupporting

confidence: 93%

“…The presence of neutral and acidic sulphated and acidic sialylated glycoconjugates (GCCs) was widely evidenced in many species including humans (Lamb and Reid 1972;Jones and Reid 1978;Jacob and Poddar 1982;Spicer et al 1983;Castells et al 1990Castells et al , 1991Castells et al , 1992Castells et al , 1994Jeffery et al 1992;K im et al 1997). Damjanov (1987) stressed the differences in the GCC composition among individual species.…”

Section: Airways' Epithelium Cholinergic Stimulation Glycoconjugatementioning

confidence: 99%

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The Effect of Intravenously Administered acetylcholine on the Glycoconjugate Composition on Goblet Cells of the Tracheal Epithelium in Rabbits

Vajner¹,

Uhlı́k²,

Konrádová³

et al. 2000

Acta Vet. Brno

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To evaluate the influence of cholinergic stimulation on the composition of glycoconjugates in goblet cells, we studied the tracheal epithelium of rabbits 5 minutes and 20 minutes after i.v. administration of acetylcholine in doses 0.1 mg and 0.5 mg, respectively. Using both conventional and lectin histochemistry, we compared the percentage of tracheal goblet cells containing neutral glycoconjugates to those containing total acidic glycoconjugates, sulphated acidic glycoconjugates, and sialylated acidic glycoconjugates. A conspicuous increase in percentage of neutral glycoconjugates-containing goblet cells and a decrease of sialylated glycoconjugatescontaining goblet cells occurred as early as 5 minutes after the administration of the lower dose and were recorded 20 minutes post exposure, too. Five minutes post exposure, the higher dose evoked a decrease in percentage of both neutral-and sialylated glycoconjugates-containing goblet cells. Twenty minutes after the administration of the higher dose, percentage of all studied elements reached values similar to those found in the lower dose group. The cholinergic stimulation did not cause total disappearance of both neutral-and sialylated glycoconjugates-containing goblet cells. Only the reaction of goblet cells 5 minutes after the administration of the higher acetylcholine dose differed quantitatively from the reactions in remaining experimental groups. Airways' epithelium, cholinergic stimulation, glycoconjugates, lectin histochemistryIn our previous studies, we demonstrated that the goblet cells (GC) in the rabbit tracheal epithelium were overstimulated and damaged due to the intravenous (i.v.) administration of acetylcholine. An apocrine type of secretion and also chain exocytosis were found after the administration of both doses of acetylcholine. Twenty minutes after the administration of 0.5 mg of acetylcholine, the degeneration of GC evoked massive differentiation of new secretory elements (Konrádová et al. 1996ab). Similar reaction of GC was also described by Specian and Neutra (1980) in the tissue culture of intestinal GC, and by Newman et al. (1996) in the tracheal epithelium of guinea pigs. Tokuyama et al. (1990) andFung et al. (1992) confirmed the stimulating effect of vagal control to the secretion of airways' GC and submucosal glands. Ramnarine and Rogers (1994) stressed non-adrenergic, non-cholinergic (NANC) neural mechanisms, coworking with neurogenic acetylcholine as "the secretion fine tuning". A direct effect of acetylcholine on tracheal secretory elements was further confirmed as a dose-dependent in studies by Kamijo et al. (1993), Woods et al. (1996), andMatera et al. (1998). Miyata et al. (1998) doubted the acetylcholine role in controlling production of high molecular weight-glycoconjugates in spite of the study by Steel and Hanrahan (1997) on GC of the tracheal epithelium in hamsters.The presence of neutral and acidic sulphated and acidic sialylated glycoconjugates

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Section: Discussionsupporting

confidence: 93%

Section: Airways' Epithelium Cholinergic Stimulation Glycoconjugatementioning

confidence: 99%

The Effect of Intravenously Administered acetylcholine on the Glycoconjugate Composition on Goblet Cells of the Tracheal Epithelium in Rabbits

Vajner¹,

Uhlı́k²,

Konrádová³

et al. 2000

Acta Vet. Brno

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“…I t is a well established laboratory animal model for studying human influenza virus infection [4], experimental teratology [5] , and modulation of airway secretion [ 6 ] . Morphologic studies of ferret airway epithelium have been limited to adult animals [7] . The purpose of this study was to describe and quantitate the postnatal development of the ferret trzcheal epithelium and submucosal glands, and thus t o determine whether the ferret is a useful model of airway epithelial and glandular development.…”

Section: Introductionmentioning

confidence: 99%

Postnatal Development of Tracheal Surface Epithelium and Submucosal Glands in the Ferret

Leigh

Gambling

Carson

et al. 1986

Experimental Lung Research

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We explored the usefulness of the postnatal ferret as a model for early developmental events in the large airways, using light and scanning electron microscopy. In the first 28 postnatal days, ferret tracheal surface epithelium and glands undergo dramatic growth and development. Tracheal surface area increases 8-fold. At birth, ciliated cells are sparse (9.4 +/- 1.2% of total epithelial cells). A significant increase in ciliated cells is observed at weekly intervals and by day 28 the ciliated cell is the predominant cell type (54.2 +/- 2.8% of total epithelial cells). Secretory cells decrease from 66.4 +/- 1.0% at birth to 22.2 +/- 2.8% of total epithelial cells. Histochemical staining of the granules of the epithelial secretory cells changes from predominantly non-acidic (staining with PAS but not Alcian blue) to predominantly acidic (staining also with Alcian blue). During the same time interval, tracheal glands develop from intraepithelial cellular aggregates devoid of secretory granules at birth into complex, submucosal tubuloacinar structures composed predominantly of cells containing non-acidic secretory granules at 28 days. Therefore, infant ferrets offer an opportunity to examine the structural and functional components of the mucociliary clearance mechanism at developmental stages which occur prenatally in many laboratory animals and in humans.

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“…The tracheobronchial tree of the ferret has abundant submucosal glands, and the epithelium of the bronchi has many mu cus-secreting cells, mainly of the serous type [Basbaum et al, 1981;Jacob and Poddar. 1982], However, the epithelium of the trachea has few or no mucus-secreting cells, and no epithelial nerves have been seen in the tracheal epithelium of this spe cies [Duffett.…”

Section: Discussionmentioning

confidence: 99%