This study investigates the polarimetric properties of skin, skeletal muscle, connective tissue, and fat using Mueller matrix imaging. It aims to compare the polarimetric characteristics of these tissues and explore how they evolve with wavelength. Additionally, the temporal evolution of certain tissues during meat aging is studied, providing insights into the dynamic behavior of polarimetric properties over time. The research employs back‐scattering configuration and the differential decomposition analysis method of Mueller matrix images. Both in‐vivo and ex‐vivo experiments were conducted using a consistent instrument setup to ensure reliable analysis. The results reveal wavelength‐dependent variations in tissue properties, including an increase in depolarization with wavelength. Significant differences in the polarimetric characteristics of meat tissues, particularly for skeletal muscle, are observed. Over a 24‐hour period, intensity, diattenuation, and retardation experience alterations, being the decreased retardation in skeletal muscle and the increased retardation in fat the most notable ones.This article is protected by copyright. All rights reserved.