Mulberrin inhibitsBotrytis cinereafor strawberry storage by interfering with the bioactivity of 14α-demethylase (CYP51)

Abstract

Currently, chemical agents hold a great promise in preventing and combating Botrytis cinerea. However, some agents' antifungal mechanism for B. cinerea remains rather vague, imposing restrictions on the research and...

Dibenzylideneacetone Overcomes Botrytis cinerea Infection in Cherry Tomatoes by Inhibiting Chitinase Activity

“…Each experiment was repeated three times. The spore growth at different concentrations was observed under an inverted microscope, and the number of spore germinations was recorded …”

“…The spore growth at different concentrations was observed under an inverted microscope, and the number of spore germinations was recorded. 4 2.7.4. TEM Observation of Mycelial Structure.…”

“…In Figure 3B, propidium iodide (PI) permeated the damaged cell membranes and was bound to intracellular DNA, emitting a vibrant red fluorescence. 4 Additionally, DCFH-DA captured intracellular ROS, oxidizing them into 2′,7′-dichlorofluorescein and generating green fluorescence under a fluorescent field. Accumulation of ROS increased mitochondrial membrane permeability, resulting in the release of Ca 2+ and subsequent apoptosis.…”

“…Botrytis cinerea stands out as one of the extensively researched fungi capable of infesting over 200 crop species, including field crops, fruits, and vegetables in temperate and subtropical regions. , This insidious invader targets various plant parts, such as roots, flowers, leaves, and fruits, leading to economic losses postharvest or during transportation. − It has been identified as one of the top 10 significant fungal plant pathogens. The disease induced by B. cinerea , termed “gray mold disease”, diminishes crop yields significantly.…”

“…As a result, the quest for novel inhibitors has become imperative. Target inhibition has emerged as a widely employed approach, primarily focusing on cell membrane enzymes (CYP51, 3-keto reductase, IPC synthase), ,− cell wall components (Chitin synthase, Chitinase), and essential enzymes involved in mitochondrial respiration (succinate dehydrogenase, cytochrome bc) for fungi. − …”