Mulberry extract ameliorates T2DM-related symptoms via AMPK pathway in STZ-HFD-induced C57BL/6J mice

Zhang, Lulu; Zhou, Xiuteng; Chen, Huijian; You, Liangzhen; Zhang, Ting; Cheng, Meng; Yao, Yudi; Pan, Xin; Yang, Xinzhou

doi:10.1016/j.jep.2023.116475

Cited by 7 publications

(4 citation statements)

References 62 publications

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“…After myotubes were fully differentiated, the cells continued to be differentiated and cultured in normal control (0.1% DMSO) and compound 3 (10, 20, and 30 μg/mL) for 48 h. After washing the cells with PBS for 3 rinses, the proteins of L6 cells were isolated, scraped off with a spatula, and then lysed using the proper kit. Relevant proteins were extracted from L6 cells based on previously reported methods. , Protein samples were divided into aliquots and then subjected to electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). When the sample is electrophoresed to the bottom of the gel, stop electrophoresis and immediately transfer the membrane.…”

Section: Methodsmentioning

confidence: 99%

“…Relevant proteins were extracted from L6 cells based on previously reported methods. 7,28 Protein samples were divided into aliquots and then subjected to electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). When the sample is electrophoresed to the bottom of the gel, stop electrophoresis and immediately transfer the membrane.…”

Section: Characterization Of Compounds Dichotominmentioning

confidence: 99%

“…Therefore, GLUT4 can be considered as a potentially effective target for the treatment of T2DM. In the research for possible hypoglycemic agents derived from natural sources, a cell-based GLUT4 translocation assay system was developed utilizing stable L6 myotubes expressing pIRAP-mOrange cDNAs to evaluate possible plant extracts and portions. − ,− During the screening of a library of plant extracts (800 biotas) with effects on GLUT4 translocation, an EtOAc fraction of C. dichotoma fruits was found to have a potentially positive activity.…”

Section: Introductionmentioning

confidence: 99%

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Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Wei,

Zhang,

Kang

et al. 2024

J. Agric. Food Chem.

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From the fruits of Cordia dichotoma, 11 new phenolic compounds, dichotomins A-K, were isolated, together with 19 known compounds. Through the analysis of detailed NMR data and HRESIMS data, the planar structures of all compounds were confirmed. Using NMR calculations, the absolute configuration of dichotomins A-K was elucidated by comparing their observed and computed electronic circular dichroism (ECD) spectra. Dichotomin H (8) and dichotomin I (9) were determined as two pairs of enantiomers. The enantiomers of compounds 8 and 9 were separated using chiral-phase high-performance liquid chromatography (HPLC), and the stereostructure of each enantiomer was determined by similarly calculating the ECD. Compounds 3, 5, 7, 17, 18, 23−25, and 27−30 increased glucose uptake by 1.04-to 2.85-folds at concentrations of 30 μg/mL. Further studies revealed that compounds 3 and 5 had a moderate effect on glucose transporter 4 (GLUT4) translocation activity in L6 cells. At 30 μg/mL, compound 3 significantly enhanced AMPK phosphorylation and GLUT4 expression. As a whole, compound 3 has the potential to be a drug candidate for the treatment of type 2 diabetes mellitus (T2DM).

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Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Compounds Dichotominmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Wei,

Zhang,

Kang

et al. 2024

J. Agric. Food Chem.

Self Cite

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“…Although several therapies targeting different mechanisms including insulin secretion, insulin sensitization, α-glucosidase inhibition, DPP-IV inhibition, GLP-1R antagonism, SGLT-2 inhibition, etc., are in use to treat diabetes, these treatment strategies have certain limitations, viz., limited efficacy and tolerance and mechanism-based toxicity, thereby posing the need to develop new molecules with better therapeutic effect. Various classes of molecules both from natural and synthetic origin, with the GLUT4 translocation stimulatory effect have been reported to regulate blood glucose levels. − Patel et al have reported a naturally occurring carbazole alkaloid koenidine with the ability to potentiate glucose utilization in the skeletal muscle and proposed it as a potential lead for diabetes management . Recently, Furuzono et al reported a xanthene derivative to attenuate glucose intolerance by inducing GLUT4 translocation in skeletal muscle in mice models .…”

Section: Introductionmentioning

confidence: 99%

Design, Synthesis, and Biological Evaluation of 1,4-Dihydropyridine–Indole as a Potential Antidiabetic Agent via GLUT4 Translocation Stimulation

Katiyar,

Ahmad,

Kumar

et al. 2024

J. Med. Chem.

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In the quest for the discovery of antidiabetic compounds, a series of 27 1,4-dihydropyridine−indole derivatives were synthesized using a diversity approach. These compounds were systematically evaluated for their antidiabetic activity, starting with an in vitro assessment for GLUT4 translocation stimulation in L6-GLUT4myc myotubes, followed by in vivo antihyperglycemic activity evaluation in a streptozotocin (STZ)-induced diabetic rat model. Among the synthesized compounds, 12,14,15,16,19,27, and 35 demonstrated significant potential to stimulate GLUT4 translocation in skeletal muscle cells. Compound 19 exhibited the highest potency and was selected for in vivo evaluation. A notable reduction of 21.6% (p < 0.01) in blood glucose levels was observed after 5 h of treatment with compound 19 in STZ-induced diabetic rats. Furthermore, pharmacokinetic studies affirmed that compound 19 was favorable to oral exposure with suitable pharmacological parameters. Overall, compound 19 emerged as a promising lead compound for further structural modification and optimization.

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Developments in the study of Chinese herbal medicine's assessment index and action mechanism for diabetes mellitus

Liu,

Zheng,

Wang

et al. 2024

Anim Models and Exp Med

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In traditional Chinese medicine (TCM), based on various pathogenic symptoms and the ‘golden chamber’ medical text, Huangdi Neijing, diabetes mellitus falls under the category ‘collateral disease’. TCM, with its wealth of experience, has been treating diabetes for over two millennia. Different antidiabetic Chinese herbal medicines reduce blood sugar, with their effective ingredients exerting unique advantages. As well as a glucose lowering effect, TCM also regulates bodily functions to prevent diabetes associated complications, with reduced side effects compared to western synthetic drugs. Chinese herbal medicine is usually composed of polysaccharides, saponins, alkaloids, flavonoids, and terpenoids. These active ingredients reduce blood sugar via various mechanism of actions that include boosting endogenous insulin secretion, enhancing insulin sensitivity and adjusting key enzyme activity and scavenging free radicals. These actions regulate glycolipid metabolism in the body, eventually achieving the goal of normalizing blood glucose. Using different animal models, a number of molecular markers are available for the detection of diabetes induction and the molecular pathology of the disease is becoming clearer. Nonetheless, there is a dearth of scientific data about the pharmacology, dose‐effect relationship, and structure–activity relationship of TCM and its constituents. Further research into the efficacy, toxicity and mode of action of TCM, using different metabolic and molecular markers, is key to developing novel TCM antidiabetic formulations.

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Mulberry extract ameliorates T2DM-related symptoms via AMPK pathway in STZ-HFD-induced C57BL/6J mice

Cited by 7 publications

References 62 publications

Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Design, Synthesis, and Biological Evaluation of 1,4-Dihydropyridine–Indole as a Potential Antidiabetic Agent via GLUT4 Translocation Stimulation

Developments in the study of Chinese herbal medicine's assessment index and action mechanism for diabetes mellitus

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