Mulberry Fruit Extract Promotes Serum HDL-Cholesterol Levels and Suppresses Hepatic microRNA-33 Expression in Rats Fed High Cholesterol/Cholic Acid Diet

Lee, Soojin; Lee, Mak-Soon; Chang, Eugene; Lee, Yoonjin; Lee, Jaerin; Kim, Jiyeon; Kim, Chong Tai; Kim, In Hwan; Kim, Yangha

doi:10.3390/nu12051499

Cited by 26 publications

(21 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, dMF had little impact on TC and LDL. These observations generally reflect mouse studies using statins and mulberry extracts that reduce obesity, 28 , 29 , 30 , 31 , 32 , 33 diabetes 34 , 35 , 36 , 37 , 38 and hyperlipidemia 21 , 30 , 39 …”

Section: Discussionsupporting

confidence: 55%

Dried mulberry fruit ameliorates cardiovascular and liver histopathological changes in high-fat diet-induced hyperlipidemic mice

Chaiwong

Chatturong

Chanasong

et al. 2021

Journal of Traditional and Complementary Medicine

View full text Add to dashboard Cite

Background and aim Metabolic disease encompasses most contemporary non-communicable diseases, especially cardiovascular and fatty liver disease. Mulberry fruits of Morus alba L. are a favoured food and a traditional medicine. While they are anti-atherosclerotic and reduce hyperlipidemic risk factors, studies need wider scope that include ameliorating cardiovascular and liver pathologies if they are to become clinically effective treatments. Therefore, the present study sought to show that freshly dried mulberry fruits (dMF) might counteract the metabolic/cardiovascular pathologies in mice made hyperlipidemic by high-fat diet (HF). Experimental procedure C57BL/6J mice were fed for 3 months with either: i) control diet, ii) HF, iii) HF+100 mg/kg dMF, or iv) HF+300 mg/kg dMF. Body weight gain, food intake, visceral fat accumulation, fasting blood glucose, plasma lipids, and aortic, heart, and liver histopathologies were evaluated. Adipocyte lipid accumulation, autophagy, and bile acid binding were also investigated. Results and conclusion HF increased food intake, body weight, visceral fat, plasma total cholesterol (TC) and low-density lipoprotein (LDL), TC/HDL ratio, blood glucose, aortic collagen, arterial and cardiac wall thickness, and liver lipid. Both dMF doses prevented hyperphagia, body weight gain, and visceral fat accumulation, lowered blood glucose, plasma TG and unfavourable TC/HDL and elevated plasma HDL beyond baseline. Arterial and cardiac wall hypertrophy, aortic collagen fibre accumulation and liver lipid deposition ameliorated in dMF-fed mice. Clinical trials on dMF are worthwhile but outcomes should be holistic commensurate with the constellation of disease risks. Here, dMF should supplement the switch to nutrient-rich from current energy-dense diets that are progressively crippling national health systems.

show abstract

Section: Discussionsupporting

confidence: 55%

Dried mulberry fruit ameliorates cardiovascular and liver histopathological changes in high-fat diet-induced hyperlipidemic mice

Chaiwong

Chatturong

Chanasong

et al. 2021

Journal of Traditional and Complementary Medicine

View full text Add to dashboard Cite

show abstract

“…This may suggest that serum TG levels may not be an accurate indicator to evaluate fatty liver disorders in animals and, probably, in humans. It could be assumed that the hepatomegaly found in the AD groups was possible due to the poor management of the dietary cholesterol metabolism [39]. Supplementation with Cur to AD did not show an improvement in the lipid profile.…”

Section: Discussionmentioning

confidence: 91%

Curcumin exerts a protective effect against obesity and liver injury induced by an atherogenic diet

Antona¹,

González²,

Ramos³

et al. 2021

FFHD

View full text Add to dashboard Cite

Background: Curcumin (Cur) is a natural yellow polyphenol extracted from the turmeric rhizome (Curcuma longa). Cur is known for its potential therapeutic properties as an analgesic, anti-inflammatory, antioxidant, antimicrobial, hepatoprotective, and anti-mutagenic, although some of these biological activities remain unproven. Epidemiological studies have shown a positive relationship between high-fat diets and diet-related chronic diseases. We hypothesized that some adverse effects of consuming atherogenic or high-fat diets (AD) can be ameliorated by Cur supplementation. Using an experimental model of rats, this study investigated the significance of Cur when it is given as a supplement in an AD.Methods: Healthy adult Wistar rats were randomly assigned to one of three groups. Controls (C) received a standard diet and experimental rats were fed with AD or AD+Cur for 5 weeks. Cur (100 mg/kg body weight) was given orally daily, plus piperine (5 mg/kg body weight). The effect of Cur supplementation was studied on zoometrics, visceral fat content, serum lipids profile, hepatosteatosis, liver function and oxidative status. Results: Diets did not alter energy consumption. As compared to the other groups, AD+Cur group showed a lower total visceral fat content, percentage of perirenal, mesenteric, and pelvic fat, and body weight gain (P< 0.05). Serum total cholesterol (P<0.0001), non-HDL-C (P<0.0001) levels were significantly higher in AD groups as compared with C. Serum triglycerides and HDL-C levels remained similar among groups (P>0.05). AD induced a liver injury with macrovesicular steatosis and portal inflammation. AD+Cur rats presented microvesicular steatosis with no inflammation, achieving the lowest level of alanine aminotransferase (ALT; P<0.0001) and reductions of aspartate aminotransferase (AST; P<0.0001). Liver homogenates from AD+Cur showed that Cur supplementation reduced the dichlorofluorescein diacetate (DCFH-DA) oxidation rate induced by AD by 25 % and deferoxamine and superoxide dismutase inhibited DCFH-DA. Conclusion: Cur as a dietary supplement showed a protective effect against obesity and inflammation, but its cardioprotective ability remained unproved. Cur may develop as a promising therapeutic agent for liver diseases induced by oxidative stress. This study provides supporting evidence to confirm the beneficial effects of curcumin from the point of view of functional food science. Keywords: curcumin, liver injury, ROS, atherogenic diet, visceral fat, obesity

show abstract

Section: Ta B L Ementioning

confidence: 99%

“…Pearson's correlation between body mass, Lee obesity index, food intake, liver HDL-C, expression of miR-33 and miR-223, and liver TC and Apo B (Lee et al, 2020(Lee et al, , 2021. Altogether, hepatic miR-33 and miR-223 expression regulate cholesterol uptake and excretion via SR-IB, HMGCR, MSMO1, SREBPs, ABCG1, and ABCA1 (Lee et al, 2020;Ye et al, 2018). However, according to our findings and other previous studies, the precise signaling pathway of the effect of AT and DE on miR-33 and miR-223 expression in liver is unclear.…”

Section: Ta B L Ementioning

confidence: 99%

Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR ‐33 and miR ‐223 expression in rat liver

Aliakbari

Saghebjoo

Sarir

et al. 2022

Journal of Food Biochemistry

View full text Add to dashboard Cite

Exercise training and medicinal herb supplementation may improve microRNAs (miRNAs) expression associated with obesity. This study aimed to assess the effects of 10 weeks of aerobic training (AT) and dill extract (DE) on miR‐33 and miR‐223 expression of liver in high‐fat diet (HFD)‐induced obese rats. Forty male Wistar rats were fed a defined high‐fat (n = 32) and standard (n = 8, nonobese control [NC]) diet. After obesity induction, obese rats were randomly allocated to four groups: AT, DE, AT + DE, and obese control (OC). Rats were euthanized and plasma and liver tissue samples were collected after the intervention. The liver expression of miR‐33 was lower in the AT, DE, AT + DE, and NC groups compared with the OC group. Also, the liver miR‐223 expression was higher in the AT, DE, AT + DE, and NC groups compared with the OC group. Moreover, the liver expression of miR‐223 in the AT + DE group was higher compared with the AT and DE groups. The AT, DE, AT + DE, and NC groups had lower liver TC compared with the OC group. Also, the plasma level of apolipoprotein B (Apo B) was significantly lower, and liver HDL‐C was significantly higher in the AT + DE and NC groups compared with the OC group. These findings show that long‐term AT combined with the intake of DE may improve the plasma levels of Apo B, and TC and HDL‐C levels in the liver, which is probably due to AT and DE positive effects on miR‐33 and miR‐223 in the liver of obese rats. Practical applications Aerobic training reduces overweight and obesity health problems, however, the duration and intensity of the exercise training distinguish between individuals. We used an integrated approach combining pharmacological and non‐pharmacological as a medical strategy to prevent HFD‐induced metabolic injury in obese rats. The present results discovered that a combination of AT + DE intervention improves the miR‐33 and miR‐223 in the liver of obese rats.

show abstract

Mulberry Fruit Extract Promotes Serum HDL-Cholesterol Levels and Suppresses Hepatic microRNA-33 Expression in Rats Fed High Cholesterol/Cholic Acid Diet

Cited by 26 publications

References 56 publications

Dried mulberry fruit ameliorates cardiovascular and liver histopathological changes in high-fat diet-induced hyperlipidemic mice

Dried mulberry fruit ameliorates cardiovascular and liver histopathological changes in high-fat diet-induced hyperlipidemic mice

Curcumin exerts a protective effect against obesity and liver injury induced by an atherogenic diet

Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR ‐33 and miR ‐223 expression in rat liver

Contact Info

Product

Resources

About