The objective of this research was to determine how mulch type affects adsorption and efficacy of commonly used preemergence herbicides in nurseries and landscapes. Nursery containers were filled with standard potting media and mulched with either pine bark (PB) mini-nuggets (mixed Pinus sp.), pine straw (PS; mixed Pinus sp.), or shredded eucalyptus hardwood (HW; Eucalyptus sp.) at a 2-inch depth. Herbicides including dimethenamid-P + pendimethalin (applied as a tank mix), prodiamine, and indaziflam were applied to mulched containers, watered in, and the mulch was subsequently removed 3 days later. Seeds of garden spurge (Euphorbia hirta), large crabgrass (Digitaria sanguinalis), and eclipta (Eclipta prostrata) were then seeded and used as bioassay species for dimethenamid-P + pendimethalin, prodiamine, and indaziflam, respectively. Chemical assays were also performed using a separate set of pots mulched with PB at 2 inches and treated with the same herbicides. Results from the bioassay experiment showed PS was the only mulch type that did not significantly decrease efficacy of any applied herbicide. Chemical assays showed only 34% of the applied dimethenamid-P reached the soil surface as evidenced by chemical assay, but more dimethenamid-P moved through PB than did pendimethalin (12%) or prodiamine (17%), which adsorbed more strongly. Overall results suggest preemergence herbicides will be strongly adsorbed to organic mulch. However, as mulch is typically more effective on weeds that germinate below the mulch layer, this does not automatically result in reduced efficacy from herbicide + mulch combinations, and the addition of a preemergence herbicide may be effective in reducing weed germination within or on top of the mulch layer.