Müller glia factors induce survival and neuritogenesis of peripheral and central neurons

Reis, Ricardo Augusto de Melo; Cabral-da-Silva, Mauricio e Castro; Mello, Fernando G. de; Taylor, Joanne

doi:10.1016/j.brainres.2008.02.035

Cited by 28 publications

(21 citation statements)

References 45 publications

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“…Previous studies that examined RGC survival in culture used embryonic animals or pups up to the second postnatal week, and in the best cases reported a 5-to 7-fold increase in RGC survival (de Melo Reis et al, 2008). To our knowledge the present study is unique in using RGCs from adult mice and rats, and we found that Otx2 promoted RGC survival 2-to 5-fold above untreated cultures.…”

Section: Discussionmentioning

confidence: 45%

Otx2 Promotes the Survival of Damaged Adult Retinal Ganglion Cells and Protects against Excitotoxic Loss of Visual AcuityIn Vivo

Ibad¹,

Rheey²,

Mrejen³

et al. 2011

J. Neurosci.

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Retinal ganglion cells (RGCs) are the projection neurons from the eye to the brain and their loss results in visual impairment in a number of diseases. Transcription factors with a homeodomain can translocate between cells and, in at least one reported case, can stimulate neuronal survival. Otx2 is a homeoprotein transcription factor expressed in the retina that is taken up by RGCs. We thus hypothesized that Otx2 capture could regulate the survival of adult RGCs. We report that Otx2 stimulates the survival of adult mouse and rat RGCs in vitro and protects RGCs against NMDA-induced toxicity in vivo in mice. In the latter model, Otx2 also preserves visual acuity.

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Section: Discussionmentioning

confidence: 45%

Otx2 Promotes the Survival of Damaged Adult Retinal Ganglion Cells and Protects against Excitotoxic Loss of Visual AcuityIn Vivo

Ibad¹,

Rheey²,

Mrejen³

et al. 2011

J. Neurosci.

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“…Our previous studies have shown that both SCs and Müller glial cells secrete multiple growth factors as well as unidentified factors which support RGC growth de Melo Reis et al 2008). This view is further supported by our present study demonstrating the switch of neurotrophic factor dependence and the enhancement of adult RGC survival and neurite outgrowth when treated with SCCM despite the relatively low survival of adult RGCs, as compared to BCF treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The purification of neonatal rodent RGCs has been well documented (Barres et al 1988;de Melo Reis et al 2008;Ito et al 1998;Jo et al 1999); however, the isolation of purified adult rodent RGCs remains yet to be demonstrated.…”

Section: Adult Rgc Purificationmentioning

confidence: 99%

“…Usefully, RGCs can be purified and grown under controlled conditions in which the effects of various factors can be determined Barres et al 1988;Goldberg et al 2002). This technique was perfected for postnatal (P) RGCs of rats (Barres et al 1988) and we have recently extended these studies in mice (de Melo Reis et al 2008). However, the technique has only been applied to these early postnatal stages.…”

Section: Introductionmentioning

confidence: 99%

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Trophic responsiveness of purified postnatal and adult rat retinal ganglion cells

Eddie

Taylor

2009

Cell Tissue Res

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Central neurons lose the ability for axonal re-growth during development and typically do not regenerate their axons following axotomy once they become mature unless given a growth-permissive environment i.e. peripheral nerve graft. In the present study, the growth responsiveness of purified retinal ganglion cells (RGCs) at different ages to neurotrophic factors and Schwann cell (SC)-secreted factors were examined directly. The purity of adult RGCs was 97% as assessed by retrograde labelling with 4,6-diamidino-2-phenylindole. The stability of cultures were demonstrated by long-term survival (30 days) in medium contained brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and forskolin (F) (BCF). RGCs from postnatal (P) (P0, P4, P8, P21) and adult (P90) rats showed decreasing levels of survival and neuritogenesis when grown in BCF. In contrast, the opposite was observed in SC-conditioned medium (CM)-treated P0-P8 RGCs which were increasingly responsive. SCCM induced maximal neurite outgrowth in P8 RGCs via the activation of extracellular regulated kinase 1/2 (Erk1/2). Inhibition of mitogen-activated protein kinase-Erk1/2 signaling using an Erk1/2-specific inhibitor (UO126) abolished SCCM-induced Erk1/2 phosphorylation and neuritogenesis completely. Although both SCCM and BCF failed to sustain the same levels of growth in P21 or P90 cultures as observed in P8 cultures, SCCM promoted higher survival and neuritogenesis than BCF-treated adult RGCs. This study is the first report of adult rat RGC purification and demonstrates that mature RGCs need multiple factors for survival and neurite outgrowth.

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“…These functions include, for example, retinal glucose metabolism; neurotransmitter recycling/mediation; maintenance of the blood-retinal barrier; maintenance of survival of photoreceptors and neurons; performance of neuronal degeneration processes; mediation of transcellular ion, water, and bicarbonate transports; mediation of the extracellular space fluid; and being a source of stem cells (Bringmann et al, 2009;Reichenbach and Bringmann, 2013). Müller cells interact with all neurons in the retinal tissue and directly take part in the regulation of synaptic activity in the inner retina (de Melo Reis et al, 2008a). Trophic factors of Müller cells can regulate numerous functions of neuronal processes such as synaptogenesis, neuroprotection, and photoreceptor survival as well as neuron-glia interactions in the retina (de Melo Reis et al, 2008b;Ruzafa and Vecino, 2015).…”

Section: Müller Cellsmentioning

confidence: 99%

Endogenous spontaneous ultraweak photon emission in the formation of eye-specific retinogeniculate projections before birth

Bókkon¹,

Scholkmann

Salari

et al. 2016

Reviews in the Neurosciences

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AbstractIn 1963, it was suggested [Sperry, R.W. (1963). Chemoaffinity in the orderly growth of nerve fiber patterns and connections. Proc. Natl. Acad. Sci. USA 50, 703–710.] that molecular cues can direct the development of orderly connections between the eye and the brain (the “chemoaffinity hypothesis”). In the same year, the amazing degree of functional accuracy of the visual pathway in the absence of any external light/photon perception prior to birth [Wiesel, T.N and Hubel, D.H. (1963). Single-cell responses in striate cortex of kittens deprived of vision in one eye. J. Neurophysiol. 26, 1003–1017.] was discovered. These recognitions revealed that the wiring of the visual system relies on innate cues. However, how the eye-specific retinogeniculate pathway can be developed before birth without any visual experience is still an unresolved issue. In the present paper, we suggest that Müller cells (functioning as optical fibers), Müller cell cone (i.e. the inner half of the foveola that is created of an inverted cone-shaped zone of Müller cells), discrete retinal noise of rods, and intrinsically photosensitive retinal ganglion cells might have key functions by means of retinal spontaneous ultraweak photon emission in the development of eye-specific retinogeniculate pathways prior to birth.

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Müller glia factors induce survival and neuritogenesis of peripheral and central neurons

Cited by 28 publications

References 45 publications

Otx2 Promotes the Survival of Damaged Adult Retinal Ganglion Cells and Protects against Excitotoxic Loss of Visual AcuityIn Vivo

Otx2 Promotes the Survival of Damaged Adult Retinal Ganglion Cells and Protects against Excitotoxic Loss of Visual AcuityIn Vivo

Trophic responsiveness of purified postnatal and adult rat retinal ganglion cells

Endogenous spontaneous ultraweak photon emission in the formation of eye-specific retinogeniculate projections before birth

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