Ship motion is an important influencing factor in passenger ship evacuation that affects the entire evacuation process by reducing individual walking speed. This study used Dalian Maritime University's training ship to conduct human walking experiments to study the influence of ship motion onNormal and fast walking speeds.It was found that during the berthing period, the individual Normal walking speed was 1.28-1.68 m/s, and the fast walking speed was 1.50-2.14 m/s. During the voyage, the ship's rolling motion reduced the Normal walking speed by 3.8%-10.3% and the fast walking speed by 3.7-14.0%. Due to the influence of ship rolling, the higher the deck and the farther away the rolling centre is, the smaller the athwartship and fore-aft walking speeds. Athwartship walking was slightly faster than fore-aft walking. In the Normal walking mode, the athwartship walking speed was 1.6%-3.7% faster than fore-aft walking, and in the fast walking mode, the athwartship walking speed was 0.8%-4.9% faster than fore-aft walking. During the berthing period, the average speed of the younger group was 24.1% higher than that of the older group. During the voyage, the reduction ratio of the individual walking speed was 86.0%-96.2%, and the value decreased as the deck height increased.