Multi-analyses of gallstones and correlation between their properties with the laboratory results

Peter, Anca; Cozmuta, Leonard Mihaly; Nicula, Camelia; Cozmuța, Anca Mihaly; Vulpoi, Adriana; Barbu–Tudoran, Lucian; Magyari, Klára; Todea, M.; Baia, Lucian; Pop, Flaviu Gheorghe

doi:10.1016/j.ab.2020.113587

Cited by 11 publications

(2 citation statements)

References 26 publications

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“…Based on the research methods and formation theories of common human body stones (such as gallstones, kidney stones and dental pulp stones) and natural apatite minerals, we explored the formation mechanism of submandibular stones ( Chatterjee et al., 2018 ; Milcent et al., 2019 ; Peter et al., 2020 ). This study introduced the theory of biomineralization and inorganic crystal variation to explain the formation mechanism of submandibular stones based on their composition, structure, and morphology.…”

Section: Discussionmentioning

confidence: 99%

The roles of heteromorphic crystals and organic compounds in the formation of the submandibular stones

Yuan

Cai

Tao

2022

Heliyon

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Section: Discussionmentioning

confidence: 99%

The roles of heteromorphic crystals and organic compounds in the formation of the submandibular stones

Yuan

Cai

Tao

2022

Heliyon

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“…The collected gallstones were thrown away materials with no human tissues or genetic material. The gallstone samples were washed with deionized water and dried at 60 ℃ in an incubator until the constant weight [21].…”

Section: Collection and Pre-treatment Of Gallstonesmentioning

confidence: 99%

In-vitro Dissolution Study of Gallstone with Medicinal Plant Extracts

Adhikari

Gyawali

2023

BIBECHANA

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Background: Gallstone disease poses a substantial economic burden on healthcare systems globally, necessitating safer alternatives to current treatments like dissolution therapy and cholecystectomy. Natural compounds from plants offer a potential solution, but research on their cholelitholytic activity is limited. In vitro dissolution studies are crucial for identifying effective plant-based therapies. Objective: This study aims to investigate the in vitro cholelitholytic activity of six plants and Ayurvedic medicines, selected based on ethnopharmacological knowledge and folk medicinal practices. Methods: Gallstone samples were categorized as combined cholesterol gallstones (CCGS) or black pigment gallstones based on external morphology and cross-sectional analysis. In vitro dissolution studies were conducted using extracts from Bergenia ciliata, Berberis asiatica, Cuscuta europaea, Kalanchoe pinnata, Teraxacum officinale, Macrotyloma uniflorum, and Ayurvedic medicines (Cystone®, Gokshuradi, and Calcury). The samples were immersed in the extracts and controls separately and incubated in a shaking water bath. The gallstone dissolution capacity was assessed by recording the dry weight of the samples at multiple time points. Results: T. officinale was highly effective in dissolving black pigment gallstones, while B. asiatica exhibited superior efficacy for CCGS. M. uniflorum and C. europaea also demonstrated significant dissolution activity against black pigment gallstones. However, K. pinnata was less effective for both gallstone types. B. ciliata and C. europaea exhibited equal effectiveness against both types. Ayurvedic medicine extracts were less effective compared to plant extracts. Conclusion: This in vitro study showed the plants can dissolve GS effectively. However, the effectiveness of the plant to dissolve GS depends on the type of the stone. The findings from this study serve as a basis for further in vivo research

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Elemental studies and mapping of cholesterol and pigment gallstones using scanning electron microscopy–energy dispersive spectroscopy

Bali,

Khajuria,

Manyar

et al. 2023

X-Ray Spectrometry

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Gallstone formation is one of the most severe human diseases, with regional differences in gallstone composition worldwide. The formation of gallstones inside the gallbladder is a complex process and is still under debate despite advances in instrumentation. This study was an in‐depth analysis of the chemical, structural, and elemental composition of cholesterol and pigment‐type gallstones using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy–energy dispersive X‐ray spectroscopy (SEM–EDS). Thermal gravimetric and differential scanning calorimetry (TG‐DSC) analysis was also carried out on gallstones to predict their thermal behavior. FTIR spectroscopy was employed to distinguish the cholesterol and pigment gallstones. Using SEM, we performed the morphological studies of gallstone and EDS were carried out to analyze elemental distribution within the gallstones. Elemental imaging and mapping of the major and minor elements within the cholesterol and black pigment gallstones were carried out, revealing the stone's heterogeneous nature. The level of heavy and toxic elements was found to be higher in pigment stones than in cholesterol gallstones. The results obtained from TG‐DSC are well correlated and supported by the results from FTIR spectroscopy.

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Multi-analyses of gallstones and correlation between their properties with the laboratory results

Cited by 11 publications

References 26 publications

The roles of heteromorphic crystals and organic compounds in the formation of the submandibular stones

The roles of heteromorphic crystals and organic compounds in the formation of the submandibular stones

In-vitro Dissolution Study of Gallstone with Medicinal Plant Extracts

Elemental studies and mapping of cholesterol and pigment gallstones using scanning electron microscopy–energy dispersive spectroscopy

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