Multi-analyte explosive detection using a fiber optic biosensor

Bakaltcheva, Irina; Ligler, Frances S.; Patterson, Charles H.; Shriver‐Lake, Lisa C.

doi:10.1016/s0003-2670(99)00571-1

Cited by 74 publications

(48 citation statements)

References 15 publications

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“…Unlabeled direct assay is a relatively quick and convenient method since it does not require the time-consuming and expensive labeling process. In order to use competitive assay, analytes or their analogs must be labeled with detectable chemicals, such as Cy5 and fluorescein (FITC) [36][37][38]. Upon incubating the analyte and labeled derivative will compete for limited binding sites of the antibodies.…”

Section: Antigens/antibodiesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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(ABSTRACT)Fiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures.To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity

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Section: Antigens/antibodiesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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“…[21] Also, a competitive fluorescence immunoassay for the detection of RDX was reported. [22] The sensitivities accomplished by these methods are, however, unsatisfactory for analyzing trace amounts of the RDX explosive.…”

mentioning

confidence: 99%

Imprinted Au‐Nanoparticle Composites for the Ultrasensitive Surface Plasmon Resonance Detection of Hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX)

2010

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The analysis of explosives attracts recent research efforts due to homeland security needs and the broad demand for the clearance of minefields. While numerous studies have addressed the development of sensing platforms for nitroaromatic explosives, and specifically trinitrotoluene (TNT), the detection of more hazardous explosives, such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) or pentaerythritol tetranitrate (PETN), is less developed and needs further efforts, particularly the improvement of the sensitivities associated with the analyses of these substrates.[1,2] Different optical, electrochemical, or microgravimetric sensors or biosensors for TNT were reported. Fluorescent organic polymers, which are quenched by nitroaromatic explosives, [3,4] luminescent polysilole nanoparticles (NPs), [5,6] or fluorescent silicon NPs quenched by nitroaromatic vapors enabled the development of optical sensors. The electrochemical activity of the nitro groups of TNT provided the basis for developing voltammetric sensors for this explosive, [7,8] and recently, a composite of Au NPs linked to electrodes enabled a sensitive electrochemical detection of TNT.[9] Also, different sensing matrices, such as cyclodextrin polymers, [10] carbowax, [11] or silicon polymers, [12] were used for TNT analysis by surface acoustic wave devices, and the aggregation of functionalized Au NPs in the presence of TNT was used to develop an optical sensor for the explosive.[13] Similarly, antibody-based optical [14][15][16] or microgravimetric quartz-crystal-microbalance [17] biosensors for TNT were developed. Different optical [18,19] or voltammetric [20] sensors for RDX were also reported. These included the fluorescence detection of RDX with an acridinium dye, [18] or the application of NADH-functionalized quantum dots.[21] Also, a competitive fluorescence immunoassay for the detection of RDX was reported.[22] The sensitivities accomplished by these methods are, however, unsatisfactory for analyzing trace amounts of the RDX explosive.Surface plasmon resonance (SPR) is a versatile method for probing changes in the refractive index occurring on thin metal films as a result of recognition events or chemical reactions. [23,24] Numerous SPR sensors and biosensors were developed, [25][26][27] and metal NPs were implemented to enhance the SPR response and to amplify SPR-based sensors. [28,29] The electronic coupling between the localized plasmon of the metallic NPs (e.g., Au NPs) and the surface plasmon wave enhances the SPR response and, thus, the labeling of recognition complexes with metallic NPs amplifies the sensing events. Different biosensing processes, such as DNA hybridization, [30] formation of immunocomplexes, [31] and the probing of biocatalytic transformations, [32] used Au NPs as labels for amplified SPR analyses. Recently, composites of bisaniline-crosslinked Au NPs were electropolymerized on Au electrodes, and the resulting matrices were used for the ultrasensitive SPR detection of TNT.[33] The formation of p-donor-acceptor complexes ...

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“…Taşınabilir fiberoptik bio sensörler patlayıcı maddeleri tanımlamak için fiber optik problara immünoanaliz uygularlar [28]. Analiz edilmiş bio sensörler TNT ve RDX gibi paylayıcıları belirli bir miktarda tespit edebilmektedirler [29]. Miktar tespiti renk değişimini gözlemleyerek ya da radyoaktivite veya flüoresanı ölçerek yapılmaktadır [55].…”

Section: Patlayıcı Algılama Metotlarıunclassified