Multi-antigenic recombinant subunit vaccine against Lawsonia intracellularis: The etiological agent of porcine proliferative enteropathy

Montesino, Raquel; Gutiérrez, Nicolás Antonio Sáez; Camacho, Frank; Farnós, Omar; Andrades, Stephanie M.; González, Alaín; Acosta, Jannel; Martı́n, Marcelo Cortez-San; Sánchez, Oliberto; Ruiz, A; Toledo, Jorge R.

doi:10.1016/j.vaccine.2019.01.029

Cited by 9 publications

(13 citation statements)

References 40 publications

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“…Pigs vaccinated with the candidate co-formulated with ppIL-18 showed a significant immune marker expression peak of the Th1 response (IL-12, TNF-α, IFN-γ, and GMCSF) at day 5 post-administration, compared with the control groups and with the group with the vaccine alone ( Figure 6 ). Although these data coincide with the increase in IL-12 reported on day 4 post-administration [ 24 ], the increase in relative expression with IL-18 is significantly higher. In vitro studies, where pig’s peripheral blood lymphocyte cultures were naturally infected with Lawsonia intracellularis , when stimulated with IL-18, showed IFN-γ increases at 24 h post-stimulation [ 65 ].…”

Section: Discussionsupporting

confidence: 83%

“…To assess the use of our IL-18 expressed in P. pastoris as a vaccine adjuvant, a vaccine candidate against L. intracellularis was used [ 24 ]. Pigs vaccinated with the candidate co-formulated with ppIL-18 showed a significant immune marker expression peak of the Th1 response (IL-12, TNF-α, IFN-γ, and GMCSF) at day 5 post-administration, compared with the control groups and with the group with the vaccine alone ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

“…The immune response of ppIL-18 with or without Lawsonia intracellularis antigens [ 24 ] was evaluated in three-week-old healthy Duroc/Yorkshire piglets. ppIL-18 and the antigens for L. intracellularis were emulsified in Montanide ISA 50 v2 (Seppic, Lons, France) with a ratio of 60:40 (water:oil).…”

Section: Methodsmentioning

confidence: 99%

“…A multi-antigenic subunit vaccine candidate against L. intracellularis has been shown to stimulate antibody production and activate the cellular immune response of vaccinated pigs, protecting them from the intestinal damage caused by the bacteria. However, antibody production occurs at week four post-immunization (1 week after the booster), even using interferon alpha as an adjuvant [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Hidalgo-Gajardo,

Gutiérrez,

Lamazares

et al. 2023

Vaccines

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Pig is one of the most consumed meats worldwide. One of the main conditions for pig production is Porcine Enteropathy caused by Lawsonia intracellularis. Among the effects of this disease is chronic mild diarrhea, which affects the weight gain of pigs, generating economic losses. Vaccines available to prevent this condition do not have the desired effect, but this limitation can be overcome using adjuvants. Pro-inflammatory cytokines, such as interleukin 18 (IL-18), can improve an immune response, reducing the immune window of protection. In this study, recombinant porcine IL-18 was produced and expressed in Escherichia coli and Pichia pastoris. The protein’s biological activity was assessed in vitro and in vivo, and we determined that the P. pastoris protein had better immunostimulatory activity. A vaccine candidate against L. intracellularis, formulated with and without IL-18, was used to determine the pigs’ cellular and humoral immune responses. Animals injected with the candidate vaccine co-formulated with IL-18 showed a significant increase of Th1 immune response markers and an earlier increase of antibodies than those vaccinated without the cytokine. This suggests that IL-18 acts as an immunostimulant and vaccine adjuvant to boost the immune response against the antigens, reducing the therapeutic window of recombinant protein-based vaccines.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Hidalgo-Gajardo,

Gutiérrez,

Lamazares

et al. 2023

Vaccines

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“…Considering that commercial vaccination is a less cost-effective strategy for controlling PPE compared with the prophylactic use of antibiotics [ 18 ], new vaccine candidate antigens have been identified, and alternative vaccine systems, such as live vector vaccines [ 26 ] or subunit vaccines with chimeric recombinant antigens [ 58 ], have been developed for use in practice. In this respect, the results of this meta-analysis and systematic review may provide industry stakeholders and researchers with updated information on the pooled effect of commercial vaccines and a summary of current scientific knowledge.…”

Section: Discussionmentioning

confidence: 99%

The Effectiveness of Commercial Vaccination against Lawsonia intracellularis in Mitigating the Reduction in ADWG, the Increased Mortality and Fecal Shedding of the Vaccinated Pigs: A Systematic Review and Meta-Analysis

Won

Chi

Park

2022

Veterinary Sciences

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In this study, a systematic review and meta-analysis was conducted to assess the efficacy of commercial vaccines against PPE in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist. Of the 373 articles reviewed, 16 fulfilled the pre-specified inclusion criteria. Three independent reviewers extracted the data, and vaccine effectiveness was assessed using the outcomes of interest. The majority of studies had a low or unclear risk of bias as assessed using the ARRIVE guidelines. The results of the meta-analysis indicated that the vaccination resulted in statistically significant reductions in bacterial fecal shedding (odds ratio, OR = 0.122, 95% confidence interval, CI 0.054–0.278) and mortality rate (risk ratio, RR = 0.199; 95% CI, 0.066–0.605). Furthermore, ADWG was significantly increased in the vaccinated pigs compared to the unvaccinated controls (standardized mean difference (SMD) = 0.606, 95% CI 0.243–0.969). In the subgroup analysis, the production phase and study type significantly influenced the effect size (p < 0.1). The Egger’s regression test showed no evidence of publication bias (p > 0.1). The effectiveness of commercially available vaccines against PPE-related weight loss, fecal shedding, and mortality suggests that the vaccines may help control PPE on affected swine farms.

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Immunity to bacterial pathogens of pigs and chickens

Foster

2021

Advancements and Technologies in Pig and Poultry Bacterial Disease Control

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Multi-antigenic recombinant subunit vaccine against Lawsonia intracellularis: The etiological agent of porcine proliferative enteropathy

Cited by 9 publications

References 40 publications

Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

The Effectiveness of Commercial Vaccination against Lawsonia intracellularis in Mitigating the Reduction in ADWG, the Increased Mortality and Fecal Shedding of the Vaccinated Pigs: A Systematic Review and Meta-Analysis

Immunity to bacterial pathogens of pigs and chickens

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