Multi‐Body Biomarker Entrapment System: An All‐Encompassing Tool for Ultrasensitive Disease Diagnosis and Epidemic Screening

Abstract

Ultrasensitive identification of biomarkers in biofluids is essential for precise diagnosis of diseases. For the gold standard approaches, polymerase chain reaction and enzyme‐linked immunosorbent assay, cumbersome operational steps hinder their point‐of‐care applications. Here, w e implement a bionic biomarker entrapment system (BioES), which employs a multi‐body Y‐shaped tetrahedral DNA probe immobilized on carbon nanotube transistors. W e successfully realize clinical identification of endometriosis by detecting an estrogen receptor, ERβ, from the lesion tissue of endometriosis patients and establish a standard diagnosis procedure. The multi‐body Y‐shaped BioES achieves a theoretical limit of detection (LoD) of 6.74 aM and a LoQ of 141 aM in a complex protein milieu. Furthermore, the BioES is optimized into multi‐site recognition module for enhanced binding efficiency, realizing the first identification of monkeypox virus antigen A35R and unamplified detection of circulating tumor DNA of breast cancer in serum. The rigid and compact probe framework with synergy effect enable the BioES to target A35R and DNA with a LoD down to 991 aM and 0.21 aM, respectively. Owing to its versatility for proteins and nucleic acids as well as ease‐of‐manipulation and ultra‐sensitivity, the BioES can be leveraged as an all‐encompassing tool for population‐wide screening of epidemics and clinical disease diagnosis.This article is protected by copyright. All rights reserved

晶体管生物传感：界面调控策略及生物医学应用

“…Nakatsuka 团队 [60] 利用二价阳离子对核酸骨架的支撑作用，开发了可以高灵敏监 结构示意图; (b) 基于 Fab 抗体的 FET 生物传感器工作原理示意图 [48] ; (c) 不同取 向的抗体在传感界面的高度和 IGZO FET 传感器的检测性能 [49] ; (d) 基于 aptamer 的 FET 传感器检测原理示意图 [27] ; (e) TDF 上端柔性悬臂摆动示意图 [50] ; (f) 基于 TDFs 的 FET 生物传感器检测原理示意图 [51] ; (g) TDFs 二聚体示意图 [52] ; (h) 二价 Y 型 TDFs 探针示意图 [53] Figure 3 illustrating the working principle of a Fab-based FET biosensor [48] ; (c) height of antibodies with different orientations at biosensing interfaces and detecting performance of IGZO FET sensors [49] ; (d) schematic diagram illustrating the detection principle of the aptamer-based FET biosensor [27] ; (e) schematic demonstrating oscillation of a flexible cantilever linked on a TDF [50] ; (f) schematic diagram illustrating the detection principle of a TDF-based FET biosensor [51] ; (g) schematic representation of TDFs dimer [52] ; (h) schematic representation of a divalent Y-shaped…”

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Solution-Induced Degradation of the Silicon Nanobelt Field-Effect Transistor Biosensors

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Label-Free and Ultrasensitive Detection of Cartilage Acidic Protein 1 in Osteoarthritis Using a Single-Walled Carbon Nanotube Field-Effect Transistor Biosensor

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