Multi-Chamber Cardioids Unravel Human Heart Development and Cardiac Defects

Schmidt, Clara; Deyett, Alison; Ilmer, Tobias; Caballero, Aranxa Torres; Haendeler, Simon; Pimpale, Lokesh; Netzer, Michael A.; Ginistrelli, Lavinia Ceci; Cirigliano, Martina; Mancheno, Estela Juncosa; Reumann, Daniel; Tavernini, Katherina; Hering, Steffen; Hofbauer, Pablo; Mendjan, Sasha

doi:10.2139/ssrn.4174579

Cited by 6 publications

(7 citation statements)

References 36 publications

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“…Currently, there are increasingly more methods for generating hCOs [ [61] , [62] , [63] ]. We should carefully choose the research model regarding to the research purpose.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

A review of protocols for engineering human cardiac organoids

Wu,

He,

et al. 2023

Heliyon

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“…Currently, there are increasingly more methods for generating hCOs [ [61] , [62] , [63] ]. We should carefully choose the research model regarding to the research purpose.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

A review of protocols for engineering human cardiac organoids

Wu,

He,

et al. 2023

Heliyon

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“… 62 For example, cardiac organoids can form ventricle-like cavities and vascular structures reminiscent of embryonic heart development, and one could imagine printing these to form more complex tissue. Although work suggests that these organoids can fuse at early stages, 63 gaps remain in our understanding of how these cells self-assemble and is untested in the context of bioprinting. A clear disadvantage of printing cardiac organoids or aggregates is their size, which typically ranges from 150 to 500 µm in diameter, which limits resolution.…”

Section: Ongoing Challenges In 3d Bioprinting Cardiac Tissuementioning

confidence: 99%

Advances in 3D Bioprinted Cardiac Tissue Using Stem Cell-Derived Cardiomyocytes

Bliley,

Stang,

Behre

et al. 2024

Stem Cells Translational Medicine

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The ultimate goal of cardiac tissue engineering is to generate new muscle to repair or replace the damaged heart. This requires advances in stem cell technologies to differentiate billions of cardiomyocytes, together with advanced biofabrication approaches such as 3D bioprinting to achieve the requisite structure and contractile function. In this concise review, we cover recent progress in 3D bioprinting of cardiac tissue using pluripotent stem cell-derived cardiomyocytes, key design criteria for engineering aligned cardiac tissues, and ongoing challenges in the field that must be addressed to realize this goal.

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“…In order to establish these parameters, ChanPharm dissociated the cardioids obtained from the consortium member (IMBA) into single cells that were subsequently characterized by means of standard patch clamp technology. The dissociation procedure is described in [74]. ChanPharm developed and refined a software package for the automatic analysis of the above-mentioned action potential parameters.…”

Section: Appendix C Measurements On Single Cardiomyocytesmentioning

confidence: 99%

“…The details of the patch clamp recordings can be found in [74]. Spontaneous electrical activity was recorded in the current clamp mode.…”

Section: Patch Clamp Recordings Of Single Cardiomyocytesmentioning

confidence: 99%

Nanoimprinted Hierarchical Micro-/Nanostructured Substrates for the Growth of Cardiomyocyte Fibers

Mühlberger,

Kopp,

Deyett

et al. 2023

Nanomanufacturing

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Investigating the behavior of cardiomyocytes is an important part of drug development. We present a structure and a related nanoimprint-based fabrication method, where the cardiomyocytes form isolated fibers, which is beneficial for drug testing, more closely representing the structure of the cardiomyocytes in vivo. We found that channel structures with walls with a rough top surface stimulate cardiomyocytes to form such fibers, as desired. Nanoimprint lithography is used as a fast and cost-efficient method to fabricate our hierarchically structured cell growth substrates.

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Multi-Chamber Cardioids Unravel Human Heart Development and Cardiac Defects

Cited by 6 publications

References 36 publications

A review of protocols for engineering human cardiac organoids

A review of protocols for engineering human cardiac organoids

Advances in 3D Bioprinted Cardiac Tissue Using Stem Cell-Derived Cardiomyocytes

Nanoimprinted Hierarchical Micro-/Nanostructured Substrates for the Growth of Cardiomyocyte Fibers

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