Multi-curvature micropatterns unveil distinct calcium and mitochondrial dynamics in neuronal networks

Khan, Hammad; Beck, Connor; Kunze, Anja

doi:10.1039/d0lc01205j

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…Control of cell positioning and neurite outgrowth direction were enabled by cell culture substrates containing microstructures or micropatterns. These microstructures could be generated by soft lithography [15][16][17][18] and laser etching [19]. There also have been direct cell patterning approach using microinjection of cells [20].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Cell Drawing Technique in Hydrogel Using Micro Injection System

Shinagawa

Miyata

2022

Micromachines

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Fabrication of three-dimensional tissues using living cells is a promised approach for drug screening experiment and in vitro disease modeling. To study a physiological neuronal function, three-dimensional cell patterning and construction of neuronal cell network were required. In this study, we proposed a three-dimensional cell drawing methodology in hydrogel to construct the three-dimensional neuronal cell network. PC-12 cells, which were used as neuronal cell differentiation model, were dispensed into a collagen hydrogel using a micro injector with a three-dimensional position control. To maintain the three-dimensional position of cells, atelocollagen was kept at sol-gel transition state during cell dispensing. As the results, PC-12 cells were patterned in the atelocollagen gel to form square pattern with different depth. In the patterned cellular lines, PC-12 cells elongated neurites and form a continuous cellular network in the atelocollagen gel. It was suggested that our three-dimensional cell drawing technology has potentials to reconstruct three-dimensional neuronal networks for an investigation of physiological neuronal functions.

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Section: Introductionmentioning

confidence: 99%

Three-Dimensional Cell Drawing Technique in Hydrogel Using Micro Injection System

Shinagawa

Miyata

2022

Micromachines

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Microcurvature landscapes induce neural stem cell polarity and enhance neural differentiation

Yuen

Yip

et al. 2023

Bio-des. Manuf.

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Nanomagnetic Guidance Shapes the Structure–Function Relationship of Developing Cortical Networks

Beck,

Killeen,

Johnson

et al. 2024

Nano Lett.

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In this study, we implement large-scale nanomagnetic guidance on cortical neurons to guide dissociated neuronal networks during development. Cortical networks cultured over microelectrode arrays were exposed to functionalized magnetic nanoparticles, followed by magnetic field exposure to guide neurites over 14 days in vitro. Immunofluorescence of the axonal protein Tau revealed a greater number of neurites that were longer and aligned with the nanomagnetic force relative to nonguided networks. This was further confirmed through brightfield imaging on the microelectrode arrays during development. Spontaneous electrophysiological recordings revealed that the guided networks exhibited increased firing rates and frequency in force-aligned connectivity identified through Granger Causality. Applying this methodology across networks with nonuniform force directions increased local activity in target regions, identified as regions in the direction of the nanomagnetic force. Altogether, these results demonstrate that nanomagnetic forces guide the structure and function of dissociated cortical neuron networks at the millimeter scale.

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Multi-curvature micropatterns unveil distinct calcium and mitochondrial dynamics in neuronal networks

Abstract: Soft-embossed highly-parallelized multi-curvature micropatterns model the impact of different curvatures (k = 0.003–0.2 μm−1) inspired by the human cerebral tissue folds on changes in spontaneous neuronal calcium signals and mitochondrial transport.

Cited by 3 publications

References 68 publications

Three-Dimensional Cell Drawing Technique in Hydrogel Using Micro Injection System

Three-Dimensional Cell Drawing Technique in Hydrogel Using Micro Injection System

Microcurvature landscapes induce neural stem cell polarity and enhance neural differentiation

Nanomagnetic Guidance Shapes the Structure–Function Relationship of Developing Cortical Networks

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