Multi-cycle operation of enhanced biological phosphorus removal (EBPR) with different carbon sources under high temperature

Shen, Nan; Chen, Yun; Zhou, Yan

doi:10.1016/j.watres.2017.02.051

Cited by 57 publications

(39 citation statements)

References 56 publications

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“…Previous studies have found uncultured Rhodocyclaceae to be dominant in the active sludge of the enhanced biological phosphorus removal reactors 40,41 . Rhodospirillaceae have been reported to be PAOs 42 ; however, another study argued that Rhodospirillaceae are glycogen-accumulating organisms 43 . As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Operation mode of a step-feed anoxic/oxic process with distribution of carbon source from anaerobic zone on nutrient removal and microbial properties

Yi-jun

Yang

et al. 2019

Sci Rep

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This study investigated the operation mode of a step-feed anoxic/oxic (A/O) process with distribution of the carbon source from the anaerobic zone in terms of the treatment effects on sewage with low carbon and high nitrogen and phosphorus. After seven phases of operation, an optimal flow distribution ratio of 75%:25% was obtained from the anaerobic zone, and the concentrations of chemical oxygen demand, ammonia nitrogen, total nitrogen, and total phosphorous in the effluent were 20.8, 0.64, 14.2, and 0.89 mg/L, respectively. The presence of an internal reflux system in the deaeration zone improved the treatment. 16S rRNA high-throughput sequencing revealed that the microbial communities in aerobic zone I(O1) of the first-step A/O sludge were different from those in aerobic zone I (O2) of the second-step A/O sludge, whereas microbial communities of the seed sludge were similar to those in O2 of the second-step A/O sludge. The richness and diversity of microbial communities in O1 of the first-step A/O sludge samples were higher than those in O2 of the second-step A/O and seed sludge. At the optimal flow distribution ratio, the microbial abundance and treatment removal efficiency were the highest.

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Section: Resultsmentioning

confidence: 99%

Operation mode of a step-feed anoxic/oxic process with distribution of carbon source from anaerobic zone on nutrient removal and microbial properties

Yi-jun

Yang

et al. 2019

Sci Rep

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“…This is of particular importance in the context of using metabolic models to study the competition between PAO and GAO, as performed by Lopez-Vazquez et al (2009). It would also enable more accurate study of PAO kinetics at wider temperature ranges, particularly in light of recent, seemingly contradictory findings concerning the population dynamics of PAO and GAO at higher temperatures (Ong et al, 2014; Shen et al, 2017). Information concerning the temperature coefficients of ( Competibacter ) GAO mirror that of PAO, their values deriving from only two investigations (López-Vázquez et al, 2007b; Lopez-Vazquez et al, 2008a).…”

Section: Discussionmentioning

confidence: 99%

Global Sensitivity Analysis of Metabolic Models for Phosphorus Accumulating Organisms in Enhanced Biological Phosphorus Removal

Quang

Rogers

Hofman

et al. 2019

Front. Bioeng. Biotechnol.

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The aim of this study was to identify, quantify and prioritize for the first time the sources of uncertainty in a mechanistic model describing the anaerobic-aerobic metabolism of phosphorus accumulating organisms (PAO) in enhanced biological phosphorus removal (EBPR) systems. These wastewater treatment systems play an important role in preventing eutrophication and metabolic models provide an advanced tool for improving their stability via system design, monitoring and prediction. To this end, a global sensitivity analysis was conducted using standard regression coefficients and Sobol sensitivity indices, taking into account the effect of 39 input parameters on 10 output variables. Input uncertainty was characterized with data in the literature and propagated to the output using the Monte Carlo method. The low degree of linearity between input parameters and model outputs showed that model simplification by linearization can be pursued only in very well defined circumstances. Differences between first and total-order sensitivity indices showed that variance in model predictions was due to interactions between combinations of inputs, as opposed to the direct effect of individual inputs. The major sources of uncertainty affecting the prediction of liquid phase concentrations, as well as intra-cellular glycogen and poly-phosphate was due to 64% of the input parameters. In contrast, the contribution to variance in intra-cellular PHA constituents was uniformly distributed among all inputs. In addition to the intra-cellular biomass constituents, notably PHB, PH2MV and glycogen, uncertainty with respect to input parameters directly related to anaerobic propionate uptake, aerobic poly-phosphate formation, glycogen formation and temperature contributed most to the variance of all model outputs. Based on the distribution of total-order sensitivities, characterization of the influent stream and intra-cellular fractions of PHA can be expected to significantly improve model reliability. The variance of EBPR metabolic model predictions was quantified. The means to account for this variance, with respect to each quantity of interest, given knowledge of the corresponding input uncertainties, was prescribed. On this basis, possible avenues and pre-requisite requirements to simplify EBPR metabolic models for PAO, both structurally via linearization, as well as by reduction of the number of non-influential variables were outlined.

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“…Accumulibacter might have a better tolerance to high temperature. Shen et al (2017) further showed that having multiple anaerobic/aerobic stages in one EBPR aided EBPR at 30 o C. Acetate as a carbon source resulted in higher process stability compared to propionate.…”

Section: Introductionmentioning

confidence: 97%

Global warming readiness: Feasibility of enhanced biological phosphorus removal from wastewater at 35°C

Qiu

Law

Zuniga-Montanez

et al. 2021

Preprint

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Recent research has shown enhanced biological phosphorus removal (EBPR) from municipal wastewater at warmer temperatures around 30oC to be stable in both laboratory-scale reactors and full-scale treatment plants. In the context of a changing climate, the feasibility of EBPR at even higher temperatures is of interest. We operated two lab-scale EBPR sequencing batch reactors with alternating anaerobic and aerobic phases for over 300 days at 30oC and 35oC, respectively, and followed the dynamics of the communities of phosphorus accumulating organisms (PAOs) and competing glycogen accumulating organisms (GAOs) using a combination of 16S rRNA gene metabarcoding, quantitative PCR and fluorescent in-situ hybridization analyses. Stable and nearly complete P removal was achieved at 30oC; similarly, long term P removal was stable at 35oC with effluent PO43-_P concentrations < 0.5 mg/L on half of all monitored days. Diverse and abundant Ca. Accumulibacter amplicon sequence variants were closely related to those found in temperate environments, suggesting that EBPR at this temperature does not require a highly specialized PAO community. The slow-feeding strategy used effectively limited the carbon uptake rates of GAOs, allowing PAOs to outcompete GAOs at both temperatures. Candidatus Competibacter was the main GAO, along with cluster III Defluviicoccus members. These organisms withstood the slow-feeding regime, suggesting that their bioenergetic characteristics of carbon uptake differ from those of their tetrad-forming relatives. This specific lineage of GAOs warrants further study to establish how complete P removal can be maintained. Comparative cycle studies at two temperatures for each reactor revealed higher activity of Ca. Accumulibacter when the temperature was increased from 30oC to 35oC, suggesting that the stress was a result of the higher carbon (and/or P) metabolic rates of PAOs and GAOs, the resultant carbon deficiency, and additional community competition. An increase in the TOC to PO43--P ratio (from 25:1 to 40:1) effectively eased the carbon deficiency and benefited the proliferation of PAOs. In general, the slow-feeding strategy and sufficiently high carbon input benefited a high and stable EBPR at elevated temperature and represent basic conditions for full-scale applications.

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Multi-cycle operation of enhanced biological phosphorus removal (EBPR) with different carbon sources under high temperature

Cited by 57 publications

References 56 publications

Operation mode of a step-feed anoxic/oxic process with distribution of carbon source from anaerobic zone on nutrient removal and microbial properties

Operation mode of a step-feed anoxic/oxic process with distribution of carbon source from anaerobic zone on nutrient removal and microbial properties

Global Sensitivity Analysis of Metabolic Models for Phosphorus Accumulating Organisms in Enhanced Biological Phosphorus Removal

Global warming readiness: Feasibility of enhanced biological phosphorus removal from wastewater at 35°C

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