Multi‐cycle recovery of lactoferrin and lactoperoxidase from crude whey using fimbriated high‐capacity magnetic cation exchangers and a novel “rotor–stator” high‐gradient magnetic separator

Abstract

BACKGROUND: Colorectal cancer (CRC) is the second leading cause of cancer death in the United States. CRC incidence and mortality rates are higher among blacks than among whites, and screening rates are lower in blacks than in whites. For the current study, the authors tested 3 interventions that were intended to increase the rate of CRC screening among African Americans. METHODS: The following interventions were chosen to address evidence gaps in the Centers for Disease Control and Prevention's Guide to Community Preventive Services: one‐on‐one education, group education, and reducing out‐of‐pocket costs. Three hundred sixty‐nine African‐American men and women aged ≥50 years were enrolled in this randomized, controlled community intervention trial. The main outcome measures were postintervention increase in CRC knowledge and obtaining a screening test within 6 months. RESULTS: There was substantial attrition: Two hundred fifty‐seven participants completed the intervention and were available for follow‐up 3 months to 6 months later. Among completers, there were significant increases in knowledge in both educational cohorts but in neither of the other 2 cohorts. By the 6‐month follow‐up, 17.7% (11 of 62 participants) of the Control cohort reported having undergone screening compared with 33.9% (22 of 65 participants) of the Group Education cohort (P = .039). Screening rate increases in the other 2 cohorts were not statistically significant. CONCLUSIONS: The current results indicated that group education could increase CRC cancer screening rates among African Americans. The screening rate of <35% in a group of individuals who participated in an educational program through multiple sessions over a period of several weeks indicated that there still are barriers to overcome. Cancer 2010. © 2010 American Cancer Society.

High-gradient magnetic separation for technical scale protein recovery using low cost magnetic nanoparticles

“…First results from a pilot plant similar to ours were published very recently and dealt with the multicycle purification of equine chorionic Gonadotropin (eCG) using microparticles [29]. Previous publications had already reported on studies carried out with a smaller rotor stator system: Brown and collaborators showed the suitability of HGMS for a technical application [30], whereas Müller and co-authors isolated eCG and compared the HGMS process with the technically established fixed-bed chromatography [31]. Our focus lies on the application of nanoparticles (instead of micro-) and also on the optimization of relevant processing parameters to improve final purification and concentration factors.…”

Use of Continuous Magnetic Extraction for removal of feedstock contaminants in flow-through mode

“…All other chemicals were of analytical grade and buffers were prepared with demineralized (pilot-scale trial) or Millipore Milli-Q system [6][7][8][9] and heat and silica treated whey (lane 10) before (lanes 2, 3, 6, 7) and after filtration (lanes 4,5,8,9) and with (lanes 3,5,7,9) and without (lanes 2, 4, 6, 8, 10) dilution 1:4 times. Lanes ultrapure water (lab-scale).…”

“…The superparamagnetic property of the adsorbent particles allows them to be separated from large suspension volumes. However of equal importance, is that when the magnetic field is turned off they can be easily resuspended in a new buffer [5]. Another unit operation tolerating feedstocks containing solids is Aqueous TwoPhase Systems (ATPS).…”

Hydrophobic and Hydrophilic Magnetite Nanoparticles: Synthesis by Chemical Coprecipitation and Physico-Chemical Characterization

“…High magnetic moment, nano-or micron-sized carriers with superparamagnetic behavior and various added functionalities provide advanced therapeutic, diagnostic or bioprocessing capabilities (Dave and Gao 2009;Reddy et al 2012). The strongly magnetoresponsive character and appropriate design of surface properties are among the main requirements for nano-or micro-adsorbents to be used in magnetic bio-separation processes, such as protein purification (Müller et al 2011) involving high gradient magnetic separation (HGMS) steps (Brown et al 2013). The building blocks for the fabrication V.-M. Socoliuc (*) · L. Vékás Romanian Academy-Timisoara Branch, Center for Fundamental and Advanced Technical Research,Mihai Viteazul Street 24,300223 Timisoara, of magnetoresponsive microcarriers are the subdomain magnetite nanoparticles surface coated by biocompatible molecular layers, such as carboxylic (lauric, myristic, oleic) acids, which ensure their stabilization and dispersion in an appropriate carrier liquid, to obtain stable magnetic nanofluids-the primary materials for the envisaged magnetic nanocomposites.…”