Multi-cycle signal-amplified colorimetric detection of tobramycin based on dual-strand displacement and three-way DNA junction

Ou, Ying; Jin, Xin; Fang, Jingwen; Tian, Yaping; Zhou, Nandi

doi:10.1016/j.microc.2020.104823

Cited by 25 publications

(4 citation statements)

References 33 publications

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“…16,17 Furthermore, compared with one-dimensional (1D) 18 and two-dimensional (2D) 19 orbital DNA nanomachines, the three-dimensional (3D) orbital DNA walker 20−22 exhibits satisfactory achievements in the field of nucleic acid amplification with high-throughput cargo loading capacity, high-density walking steps, and faster target recovery rates. Therefore, through the justifiable design of the orbit, the accurate identification and multicycle amplification 23,24 of target miRNA can be realized, and the intensity of ECL signal response can be improved vigorously.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Nevertheless, as a special species of nanomachines, inspired by the biological protein motors, a DNA walker can amplify the signal by providing energy to drive the walking chain, moving along a specific track, which further improves its operability, accuracy, and flexibility. − In the process of walking, driven by the strand-displacement reaction, the DNA walker can move autonomously along the predetermined orbit through a swing arm probe and track probe, improving the local concentration and achieving the accumulation of signal. , Furthermore, compared with one-dimensional (1D) and two-dimensional (2D) orbital DNA nanomachines, the three-dimensional (3D) orbital DNA walker − exhibits satisfactory achievements in the field of nucleic acid amplification with high-throughput cargo loading capacity, high-density walking steps, and faster target recovery rates. Therefore, through the justifiable design of the orbit, the accurate identification and multicycle amplification , of target miRNA can be realized, and the intensity of ECL signal response can be improved vigorously.…”

Section: Introductionmentioning

confidence: 99%

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3D DNA Walker-Assisted CRISPR/Cas12a Trans-Cleavage for Ultrasensitive Electrochemiluminescence Detection of miRNA-141

et al. 2021

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In this study, a CRISPR/Cas12a (LbCpf1)mediated electrochemiluminescence (ECL) paper-based platform on the basis of a three-dimensional (3D) DNA walker was proposed for the ultrasensitive detection of miRNA-141. Initially, 3D-rGO with a tremendous loading space was modified on the paper working electrode (PWE) to construct an excellent conductive substrate and facilitate the growth of AuPd nanoparticles (NPs). Afterward, the AuPd NPs were introduced as the coreaction emitter medium of the 3D-rGO/PWE to provide convenience for the transformation between S 2 O 8 2− and SO 4 2− , amplifying the ECL emission of g-C 3 N 4 nanosheets (NSs). Meanwhile, with the help of Nt.BsmAI nicking endonuclease, a 3D DNA walker signal amplifier was designed to convert and magnify the target miRNA-141 into a particular trigger sequence, which could act as activator DNA to motivate the trans-acting deoxyribonuclease activity of CRISPR/Cas12a to further achieve efficient annihilation of the ECL signal. Furthermore, the proposed multimechanism-driven biosensor exhibited excellent sensitivity and specificity, with a relatively low detection limit at 0.331 fM (S/ N = 3) in the concentration range between 1 fM and 10 nM. Consequently, the designed strategy not only extended the application scope of CRISPR/Cas12a but also devoted a new approach for the clinical diagnosis of modern medicine.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D DNA Walker-Assisted CRISPR/Cas12a Trans-Cleavage for Ultrasensitive Electrochemiluminescence Detection of miRNA-141

et al. 2021

View full text Add to dashboard Cite

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“…Ou et al used a multi-cycle signal amplification strategy and incorporated with double-strand displacement and DNA three-way ligation structure to establish a highly sensitive and selective colorimetric sensor for TOB. The LOD of this method was 12.24 nmol/L and the linear range was 20 nmol/L–800 nmol/L [ 121 ]. The detection process avoids the unfavorable factors of expensive consumables and complex preparation.…”

Section: Affinity-based Analysis Methods For Aminoglycoside Residuesmentioning

confidence: 99%

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Liu

Chen

et al. 2023

Foods

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With the increasingly serious problem of aminoglycoside antibiotic residues, it is imperative to develop rapid, sensitive and efficient detection methods. This article reviews the detection methods of aminoglycoside antibiotics in animal-derived foods, including enzyme-linked immunosorbent assay, fluorescent immunoassay, chemical immunoassay, affinity sensing assay, lateral flow immunochromatography and molecular imprinted immunoassay. After evaluating the performance of these methods, the advantages and disadvantages were analyzed and compared. Furthermore, development prospects and research trends were proposed and summarized. This review can serve as a basis for further research and provide helpful references and new insights for the analysis of aminoglycoside residues. Accordingly, the in-depth investigation and analysis will certainly make great contributions to food safety, public hygiene and human health.

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“…In general, aptamers and ribozymes are two types of nucleic acid recognition elements for non-nucleic acid biomarkers. In this section, we mainly introduce nucleic acid recognition elements for the detection of small molecules, ,,− proteins, ,,− cells, and metal ions ,− based on aptamers and ribozymes (Figure ).…”

Section: Clinical Translation-driven Nucleic Acid-based Testsmentioning

confidence: 99%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are natural biopolymers composed of nucleotides that store, transmit, and express genetic information. Overexpressed or underexpressed as well as mutated nucleic acids have been implicated in many diseases. Therefore, nucleic acid tests (NATs) are extremely important. Inspired by intracellular DNA replication and RNA transcription, in vitro NATs have been extensively developed to improve the detection specificity, sensitivity, and simplicity. The principles of NATs can be in general classified into three categories: nucleic acid hybridization, thermal-cycle or isothermal amplification, and signal amplification. Driven by pressing needs in clinical diagnosis and prevention of infectious diseases, NATs have evolved to be a rapidly advancing field. During the past ten years, an explosive increase of research interest in both basic research and clinical translation has been witnessed. In this review, we aim to provide comprehensive coverage of the progress to analyze nucleic acids, use nucleic acids as recognition probes, construct detection devices based on nucleic acids, and utilize nucleic acids in clinical diagnosis and other important fields. We also discuss the new frontiers in the field and the challenges to be addressed.

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Multi-cycle signal-amplified colorimetric detection of tobramycin based on dual-strand displacement and three-way DNA junction

Cited by 25 publications

References 33 publications

3D DNA Walker-Assisted CRISPR/Cas12a Trans-Cleavage for Ultrasensitive Electrochemiluminescence Detection of miRNA-141

3D DNA Walker-Assisted CRISPR/Cas12a Trans-Cleavage for Ultrasensitive Electrochemiluminescence Detection of miRNA-141

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Nucleic Acid Tests for Clinical Translation

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