Multi-drug Resistant Mycobacterium leprae from Patients with Leprosy.

Abstract

Sequences of the folP1, rpoB, and gyrA genes were analyzed for 88 isolates of Mycobacterium leprae from leprosy patients in Japan, Haiti, Indonesia, Pakistan, and the Philippines. Thirteen isolates (14.8%) showed representative mutations in more than two genes, suggesting the emergence of multidrug-resistant M. leprae.

Quinolones for mycobacterial infections

“…This leads to the conclusion that FQ resistance in M. leprae is present but not common and is observed mostly in cases that failed therapy [38].…”

“…In an earlier survey from Japan, 88 strains of M. leprae were studied; 11 strains showed resistance located on two different genes, and 2 isolates had three gene mutations conferring resistance; in all these cases FQ resistance was involved. The origin of the strains was from the countries mentioned above [38].…”

Multi-targeted therapy for leprosy: Insilico strategy to overcome multi drug resistance and to improve therapeutic efficacy

“…Though global efforts were taken to control leprosy by intensive chemotherapy, it is still a major health problem in several countries of Asia, Latin America, and Africa. M. leprae develops resistance since 1964 for Dapsone, 1976 for Rifampin, and 1996 for Ofloxacin (Nakata et al, 2011(Nakata et al, , 2012Rocha Ada et al, 2012;Maeda et al, 2001).…”

A Mutation at Codon 516 in the rpoB Gene of Mycobacterium leprae Confers Resistance to Rifampin

“…Approximately 12 months after inoculation, bacterial suspension for drug susceptibility testing was prepared from the nude mice footpads, which had shown bacillary multiplication. Drug susceptibility testing, for dapsone, rifampin, ofloxacin, sparfloxacin, clofazimine, and clarithromycin was per-formed in the same manner as previously presented ( 11,12,13 ). Additionally , 0.08% minocycline ( 3 ) was added to the drug group for susceptibility testing in the present study .…”

“…Direct sequencing of the PCR products was performed with the ABI Prism 310 Genetic Analyzer (Perkin-Elmer Applied Biosystems, Norwalk). Sequencing data was analyzed by the DNASIS program (Hitachi Software Engineering, Yokohama, Japan), as presented elsewhere ( 9,11,14 ). The DNA sequence was compared with that in the GenBank database.…”