Multi‐enzymatic recovery of fungal cellulases (Aspergillus niger) through solid‐state fermentation of sugarcane bagasse

Abstract

In this study, the optimum conditions for the multi‐enzymatic recovery of cellulases produced by Aspergillus niger were investigated using sugarcane bagasse. Two extraction methods were investigated: the two‐stage solid–liquid extraction (SLE) followed by ultrasound‐assisted extraction (UAE), and the single‐stage SLE. The ultrasound effects were evaluated using a Doehlert design, in which the pH (5.0–9.0) and sonication power (0.8–2.0 W ml−1) were independent variables. For the single‐stage SLE, temperature (25–45°C), time (10–60 min), and pH (5.0–9.0) were analyzed using the Box–Behnken design. Both processes were monitored to evaluate FPase, CMCase, and β‐glucosidase (U ml−1) activities. The maximum enzymatic activities (EA) obtained for FPase, CMCase, and β‐glucosidase in the SLE–UAE were 0.352, 0.321, and 1.412 U ml−1, respectively. Unlike in most previous studies, sonication was insignificant (p < 0.05) with respect to the enzymatic complex within the evaluated ranges. Moreover, sonication changed the EA when lower than 1.2 and higher than 1.6 W ml−1, mainly inhibiting the EA of β‐glucosidase. The single‐stage SLE was more effective than the two‐stage SLE–UAE, and the maximum EA values for FPase, CMCase, and β‐glucosidase were 0.354, 0.303, and 3.135 U ml−1, respectively. The single‐stage process was better because it consumed less energy, required simpler equipment, and provided higher efficiency in a shorter time. This study will improve diversified enzyme extraction from sugarcane bagasse, reduce enzyme production costs, and enhance bagasse utilization.

Xylanase production by Penicillium sp. Pn004 and its application for grass hydrolysis: high value subproduct from non-centrifugal sugarcane bagasse and wheat bran

“…These results highlight the importance of buffer solutions on enzyme shelf life to mitigate the loss of catabolic activity due to changes in pH and ionic strength of the solution over time, which shall affect the shape of the active site and its solubility (Chapman et al, 2019;Eun, 1996). (Adhyaru et al, 2016;Alves et al, 2022;Jonathan et al, 2021). Thus, an experiment was set up to study the effect of the pH and the type of the extraction solution.…”

Fungal Growth and Pathology

“…Most fungi survive and reproduce between pH 3 and 10. The optimum pH range is between 5 and 7 [20][21][22]. In addition, if the environment in which fungi are found in alkaline, they can achieve maximum growth by converting the pH of the environment to the optimum growth pH with the organic acids they secrete.…”

Division mechanism of labor in Diqing Tibetan Pigs gut microbiota for dietary fiber efficiently utilization

“…Presently, the fungi used in cellulase production mainly include Trichoderma, Penicillium and Aspergillus(Li et al, 2022;Ogunyewo et al, 2021;Fan et al, 2022). However, the long growth cycle and harsh culture conditions of fungi lead to high enzyme-production cost, which limits the widespread use of cellulase(Alves et al, 2022). Therefore, the isolation of breeding strains with a short growth cycle and high enzyme-production e ciency for ber degradation, that can be cultivated at low cost, has become an urgent scienti c problem.…”