Multi‐excitation fluorescence spectroscopy for analysis of non‐alcoholic fatty liver disease

Sauvage, Vincent; Levene, Adam P; Nguyen, Hoa; Wood, Tobias C.; Kudo, Hiromi; Concas, Danilo; Thomas, Howard; Thursz, Mark; Goldin, Robert; Anstee, Quentin M.; Elson, Daniel S.

doi:10.1002/lsm.21064

Cited by 13 publications

(14 citation statements)

References 31 publications

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“…1 Typically, such a probe incorporates a light source, fiber optic cables for light transport, and an optical detector integrated with spectroscopic devices. Different spectroscopic fiber optic probes have long since been developed and used to substantially improve clinical diagnostic capability for different purposes, 2,3 such as surgical guidance during brain tumor resection, [4][5][6] determination of tissue optical property for optical biopsy of brain tissue 7 and for interstitial photodynamic therapy (PDT), 8 treatment monitoring during PDT, 9,10 lung cancer staging of mediastinal lymph nodes, 11 diagnosis of nonalcoholic fatty liver disease, 12 detection of metastatic breast cancer in sentinel lymph nodes, 13 and tumor oxygenation monitoring and tissue optical properties evaluation for breast cancer diagnosis. 14,15 These probes have the flexibility that they can be manufactured into either cavities or tubular structures, put in contact with epithelial surfaces, or inserted into structures, punctured by rigid devices such as needles.…”

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confidence: 99%

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Xie

Mousavi

et al. 2014

J. Biomed. Opt

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Abstract. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring.

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confidence: 99%

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Xie

Mousavi

et al. 2014

J. Biomed. Opt

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“…The changes that arise as a result of renal failure affect almost every body system, which can cause changes in the fluorescent parameters of urine. [4] Studies of urine fluorescent characteristics of children suffering from various urinary tract diseases, such as glomerulonephritis, nephritic syndrome, and chronic renal insufficiency, showed differences between the urine fluorescence of patients and healthy children. [5] In the urine CM of dogs with chronic renal failure (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…[1] FS (fluorescence spectroscopy) is a potential diagnostic technique for many disorders. [2] Successful diagnostic application of this technique could be achieved because autofluorescence alterations of human tissues [2][3][4] or body fluids [5,6] are caused by pathological process. Some studies were also performed in animal models.…”

Section: Introductionmentioning

confidence: 99%

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Pilot Study of Canine Urine Analysis Using Fluorescent Fingerprint

Šteffeková

Birková

Baranová

et al. 2015

Spectroscopy Letters

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Urine contains a variety of compounds including a number of natural fluorophores. Fluorescence spectroscopy has proven to be a useful tool in analytical science. Fluorescence detection has three major advantages over other light-based investigation methods: high sensitivity, speed, and safety. Our work presents the newest approach to analysis of dog urine. Fluorescent fingerprint can very quickly reveal differences between complicated mixtures without adding any reagents. We describe autofluorescence characteristics of the urine of healthy dogs and of those with various disorders using fluorescent fingerprint. Fluorescent analysis has given good results to distinguish between pathological urine and the healthy standard in dogs. Our results suggest that this method can be used to characterize fluorescent properties of canine urine and to reveal some pathological changes in dogs.

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“…While most of the LIF studies have focused on differentiating malignant and normal tissues in disease diagnosis, 15 there are existing studies on the variation of tissue fluorescence due to temperature and other thermo-transformations. For instance, Lin et al 16 studied the use of fluorescence measurements to monitor thermal damage induced on canine liver samples, which were subjected to isothermal baths at different temperatures and for different incremental durations.…”

Section: Introductionmentioning

confidence: 99%

Laser-induced tissue fluorescence in radiofrequency tissue-fusion characterization

Fonseca

Arya

et al. 2014

J. Biomed. Opt

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Abstract. Heat-induced tissue fusion is an important procedure in modern surgery and can greatly reduce trauma, complications, and mortality during minimally invasive surgical blood vessel anastomosis, but it may also have further benefits if applied to other tissue types such as small and large intestine anastomoses. We present a tissue-fusion characterization technology using laser-induced fluorescence spectroscopy, which provides further insight into tissue constituent variations at the molecular level. In particular, an increase of fluorescence intensity in 450-to 550-nm range for 375-and 405-nm excitation suggests that the collagen cross-linking in fused tissues increased. Our experimental and statistical analyses showed that, by using fluorescence spectral data, good fusion could be differentiated from other cases with an accuracy of more than 95%. This suggests that the fluorescence spectroscopy could be potentially used as a feedback control method in online tissue-fusion monitoring.

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Multi‐excitation fluorescence spectroscopy for analysis of non‐alcoholic fatty liver disease

Abstract: Background and objectives: the increasing incidence of non-alcoholic fatty liver diseases

Cited by 13 publications

References 31 publications

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Pilot Study of Canine Urine Analysis Using Fluorescent Fingerprint

Laser-induced tissue fluorescence in radiofrequency tissue-fusion characterization

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