Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Abstract

Responsive two-dimensional photonic crystal (2DPC) hydrogels have been widely used as smart sensing materials for constructing various optical sensors to accurately detect different target analytes. Herein, we report photonic hydrogel aptasensors based on aptamer-functionalized 2DPC poly(acrylamide-acrylic acid-N-tert-butyl acrylamide) hydrogels for facile, label-free and colorimetric detection of lysozyme in human serum. The constructed photonic hydrogel aptasensors undergo shrinkage upon exposure to lysozyme solution through multi-factors cooperative actuation. Here, the specific binding between the aptamer and lysozyme, and the simultaneous interactions between carboxyl anions and N-tert-butyl groups with lysozyme, increase the cross-linking density of the hydrogel, leading to its shrinkage. The aptasensors’ shrinkage decreases the particle spacing of the 2DPC embedded in the hydrogel network. It can be simply monitored by measuring the Debye diffraction ring of the photonic hydrogel aptasensors using a laser pointer and a ruler without needing sophisticated apparatus. The significant shrinkage of the aptasensors can be observed by the naked eye via the hydrogel size and color change. The aptasensors show good sensitivity with a limit of detection of 1.8 nM, high selectivity and anti-interference for the detection of lysozyme. The photonic hydrogel aptasensors have been successfully used to accurately determine the concentration of lysozyme in human serum. Therefore, novel photonic hydrogel aptasensors can be constructed by designing functional monomers and aptamers that can specifically bind target analytes.

Smart Two-dimensional Photonic Crystal Hydrogel for Accurate Detection of Hg2+

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“…近年来, 不同类型的智能二维光子晶体水凝胶已被 报道. 例如, Meng 等、Wu 等以及本课题组分别报道了 适配体功能化的智能二维光子晶体水凝胶, 实现了对病 毒 [12][13] 、半胱氨酸 [14] 、黄曲霉毒素 [15] 、腺苷 [16] 、蛋白 酶 [17][18] 等的检测. Cai 等 [19] 制备了一种无标记的辣根过 氧化物酶/牛血清白蛋白复合二维光子晶体水凝胶, 用 于检测 H 2 O 2 .…”

Host–Guest Interaction and Hydrogen Bonding Cross-Linking Actuated Smart Two-Dimensional Photonic Hydrogel Sensor of l-Histidine

“…The measurement of the Debye ring diameter is facile and can be performed using only a ruler and a laser pointer. The simple fabrication of 2DPC, the easy functionalization of hydrogels, and convenient measurement of Debye ring diameter endow the 2DPCH sensors with wide use in sensing antibiotics, , proteases, , viruses, and metal ions . Presently, it has been reported that 2DPCH has been developed to detect amino acids, , in which aptamers were used as recognition molecules and were copolymerized into the hydrogel.…”

Review on Carbon Dot-Based Fluorescent Detection of Biothiols

“…[ 35 , 36 , 37 , 38 , 39 , 40 ]. Recently, a smartphone-based surface plasmon-coupled emission (SPCE) platform and photonic crystal-coupled emission (PCCE) technology were also employed in biothiol quantification as well as in biosensing studies [ 41 , 42 , 43 , 44 , 45 , 46 ]. Among these materials, functionalized fluorescent quantum dots have attracted much attention due to their size, photostability, and unique optical properties (Stokes shifts, wide absorption and optimizable PL, etc.)…”