Multi-feedstock biorefinery concept: Valorization of winery wastes by engineered yeast

Baptista, Sara Isabel Leite; Romaní, Aloia; Cunha, Joana T.; Domingues, Lucı́lia

doi:10.1016/j.jenvman.2022.116623

Cited by 12 publications

(7 citation statements)

References 100 publications

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“…The C/N ratios for each formulation are indicated 2. The pH of each media was adjusted to 6.8-7.0 with KOH (5 mol.L −1 ), and 6.5 g/L CaCO 3 was added to maintain the culture pH during fermentation [27,28].…”

Section: Media Composition and Preparationmentioning

confidence: 99%

Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

Francisco,

Aguiar,

Abreu

et al. 2023

Fermentation

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In this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain from a set of engineered strains was identified in synthetic media mimicking the composition of the non-detoxified EBH (SM), the lipid profile was characterized, and yeast extract and corn steep liquor (CSL) were pinpointed as supplements enabling a good balance between lipid accumulation, biomass production, and autolysis by A. gossypii. The potential of the engineered A. gossypii A877 strain to produce lipids was further validated and optimized with minimally processed inhibitor-containing hydrolysate and high sugar concentration, and scaled up in a 2 L bioreactor. Lipid production from non-detoxified EBH supplemented with CSL reached a lipid titer of 1.42 g/L, paving the way for sustainable single-cell oil production within the concept of circular economy and placing lipids as an alternative by-product within microbial biorefineries.

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Section: Media Composition and Preparationmentioning

confidence: 99%

Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

Francisco,

Aguiar,

Abreu

et al. 2023

Fermentation

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“…6.8 million hL (Santos et al, 2022a). In the winemaking process, significant quantities of by-products are generated, both liquid, as washing waters, and solid, namely, stalk (5%-7%), pomace (20%-25%), and lees (2%-6%) (Prozil et al, 2012;Baptista et al, 2023). In fact, for producing 100 L of wine, ca.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, for producing 100 L of wine, ca. 31 kg of solid and 50-140 L of liquid by-products are generated (Prozil et al, 2012;Baptista et al, 2023). One of the biggest issues associated with winemaking residues is the environmental impact of improper disposal.…”

Section: Introductionmentioning

confidence: 99%

Binderless particleboards obtained 100% from winery by-products for the packaging industry

Fernandes,

Lopes,

Ferreira

et al. 2024

Front. Food. Sci. Technol.

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The valorization of bioeconomy by-products holds immense significance in achieving sustainability goals and fostering resource efficiency, not only to reduce the amount of waste generated but also to significantly decrease the carbon footprint associated with several industrial fields, by promoting circular economy. The wine industry is not only one of the major contributors for world’s economy but also a great producer of by-products, with no valorization process associated. Grape stalks are a great source of chemical molecules, as polyphenols, that can be applied in the production of bio-adhesives. In the present work, grape stalk particles were used as raw material to obtain a highly rich extract with bonding properties through solid/liquid extraction. Different extraction agents were tested (water, NaOH, and Na2CO3) at varied concentrations (0.1%, 0.5%, and 1.0%, respectively). Additionally, extracts were chemically characterized, and the bonding capacity was also evaluated. Using NaOH 1.0%, an extraction efficiency of 8.9% and a total content of polyphenols of 1.4% were achieved. Moreover, all extracts presented an interesting bonding capacity (>1.0 MPa) by hot-press at 120°C for 120 s. The composite panel produced using grape stalk adhesive and vine strain particles fulfills the requirements for type P1 particleboards in terms of internal bond strength, reaching 0.66 MPa. The mechanical and wettability properties of composite material allow foreseeing a promising application in the food packaging industry.

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“…GM is rich in fructose and glucose (in a ratio of roughly 1:1) and also contains organic acids, nitrogen compounds, minerals, polyphenols, vitamins and aromatic compounds. 18,19 This low-cost by-product, whose bulked price was barely €0.3037 L −1 in Spain in 2019, 19 was used as feedstock for the biosynthesis of GA by A. niger mutant strains, [20][21][22][23] mannitol by Lactobacillus intermedius 19 and bioethanol by engineered Saccharomyces cerevisiae, 24 among other added-value chemicals.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of gluconic acid production by Aspergillus niger from by‐products as glucose source using pressurized air conditions

Fernandes

Dias

Belo

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND: The profitable production of gluconic acid (GA), an organic acid extensively used in the food and beverages industries, relies on the use of cheap and available substrates with high sugar content. The high oxygen demand for the microbial conversion of glucose to GA is still a bottleneck of industrial production in conventional stirred-tank reactors. Therefore, in this study, for the first time, the effect of increased air pressure up to 4 bar on GA production and sugar consumption in batch and step-wise fed-batch cultures in sugarcane molasses (ScM) and grape must (GM) medium was studied.RESULTS: ScM and GM, by-products of the agro-food industry, proved to be adequate feedstocks to replace pure glucose in GA batch production by Aspergillus niger MUM 92.13. A threefold improvement in GA productivity was obtained in batch experiments carried out at 4 bar with ScM and GM compared to those at 1 bar. Furthermore, the addition of two pulses of ScM, under pressurized conditions, increased the GA production, reaching a final concentration of 140 g L −1 . CONCLUSION: This work proved that the use of total air pressure up to 4 bar is an effective approach to enhance GA productivity using ScM and GM as substrates in batch and step-wise fed-batch cultures. Also, this study provides a novel strategy for developing a biotechnological bioprocess for GA production by A. niger from low-cost and sugar-rich by-products performed in pressurized stirred tank reactors.

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Multi-feedstock biorefinery concept: Valorization of winery wastes by engineered yeast

Cited by 12 publications

References 100 publications

Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

Binderless particleboards obtained 100% from winery by-products for the packaging industry

Enhancement of gluconic acid production by Aspergillus niger from by‐products as glucose source using pressurized air conditions

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