Multi-function Plant Defensin, Antimicrobial and Heavy Metal Adsorbent Peptide

Abstract

Background: Defensin peptide isolated from plants are often heterogeneous in length, sequence and structure, but they are mostly small, cationic and amphipathic. Plant defensins exhibit broad-spectrum antibacterial and antifungal activities against Gram-positive and Gram-negative bacteria, fungi and etc. Plant defensins also play an important role in innate immunity, such as heavy metal and some abiotic stresses tolerance. Objectives: In this paper, in vitro broad-spectrum activities, antimicrobial and heavy metal absorption, of a recombinant plant defensin were studied. Material and Methods: SDmod gene, a modified plant defensin gene, was cloned in pBISN1-IN (EU886197) plasmid, recombinant protein was produced by transient expression via Agroinfiltration method in common bean. The recombinant protein was tested for antibacterial activity against Gram-negative, Gram-positive bacteria and Fusarium sp. the effects of different treatments on heavy metal zinc absorption by this peptide were tested. Results: We confirmed the antibacterial activities of this peptide against Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Bacillus cereus) bacteria, and antifungal activities of this peptide against Fusarium spp. (Fusarium oxysporum and Fusarium solani). High metal absorption coefficient for this peptide was also observed. Results: Out of six actinobacterial isolates, VITVAMB 1 possessed the most efficient RO-16 decolorization property. It decolorized 85.6% of RO-16 (250 mg L -1 ) within 24hrs. Isolate VITVAMB 1 was identified to be Nocardiopsis sp. Maximum dye decolorization occurred at pH 8, temperature 35 o C, 3% salt concentration and a dye concentration of 50 mg L -1 . Conclusions: Results suggesting that modified defensin peptide facilitates a broader range of defense activities. dedefensins are an important part of the innate immune system in eukaryotes. These molecules have multidimensional properties that making them promising agents for therapeutic drugs.

Effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/STAT3/Survivin pathway

“…MicroRNAs play an important role in this regard (31). There are some factors that stimulate the expression of genes in plants, or by producing transgenic plants and using the engineering method to create metabolites (32)(33)(34)(35). In this research, we investigate the effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/ STAT3/Survivin pathway.…”

RNA-Sequencing in Resistant (QL3) and Susceptible (Theis) Sorghum Cultivars Inoculated With Johnsongrass Isolates of Colletotrichum sublineola

“…The most extreme cases include a lichenase-2 precursor, which was up 10.8-fold in QL3 and only 3.9-fold in Theis. Lichenases are involved in the hydrolysis of D glucans in plant cell walls during growth, and a lichenase has been shown to inhibit the growth of Fusarium in vitro ( Mirakhorli et al, 2019 ). A lichenase has also been shown to be differentially upregulated in potatoes in response to Alternaria alternata ( Brouwer et al, 2020 ).…”

Plant-Derived Antimicrobial Peptides: A Plant Defense Weapon against Biotic and Abiotic Stresses

“…Although the roles of PAMPs in the suppression of bacteria and fungi have been extensively investigated [30][31][32] their roles in reducing the negative effects of abiotic stresses appear to be neglected. In fact, not many papers have been found to work on the functions of PAMPs in improving plant tolerance to salinity and drought stress [33,34], heavy metals [35], and wound stress [36]. Transgenic crops have been developed over the past decades to integrate beneficial genes from bacteria, fungi, and other plant species into the host plant genomes.…”