Multi-generation studies with Chironomus riparius – Effects of low tributyltin concentrations on life history parameters and genetic diversity

Vogt, Christian; Nowak, Carsten; Diogo, João Barateiro; Oetken, Matthias; Schwenk, Klaus; Oehlmann, Jörg

doi:10.1016/j.chemosphere.2006.12.025

Cited by 73 publications

(67 citation statements)

References 18 publications

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“…as a result of low contaminant exposure (Vogt et al 2007b), are threatened with extinction if a second stressor, such as slightly altered temperatures, comes into force. These results have considerable implications for the distribution and survival of locally distributed or rare species.…”

Section: Resultsmentioning

confidence: 99%

Interaction between genetic diversity and temperature stress on life-cycle parameters and genetic variability in midge Chironomus riparius populations

Vogt

Pupp²,

Nowak³

et al. 2007

Clim. Res.

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The genetic diversity of natural populations and the increase in global temperature are becoming important issues in ecotoxicological research. We investigated the combined effects of 3 different temperature levels and 6 different levels of genetic diversity on the life history parameters of Chironomus riparius. Inbreeding and loss of genetic variation are major threats to small and endangered populations. The reduction of fitness due to inbreeding is more severe under stressful environmental conditions. We show that under even a moderate temperature stress, a decrease in genetic variation has important consequences on the fitness of populations. This indicates that the global temperature increase could have serious effects on the distribution and survival of locally distributed or rare species.KEY WORDS: Life-history parameters · Microsatellites · Diptera · Chironomidae · Genetic impoverishment Resale or republication not permitted without written consent of the publisherClim Res 33: [207][208][209][210][211][212][213][214] 2007 The aim of this study was to determine the interaction between temperature stress and inbreeding, using an ectothermic aquatic invertebrate to test if genetically impoverished populations exhibit a higher sensitivity to temperature shifts. We selected a species from the dipterian family of Chironomidae, the non-biting midge Chironomus riparius, representing a model organism with a benthic-associated life cycle. Chironomids are widely distributed in the temperate northern hemisphere and are characterised by a huge ecological tolerance (Armitage et al. 1995). They can be found in hot springs, eutrophic and oligotrophic ponds, lowland rivers, and sub-glacial streams (Pinder 1986). Chironomids have 3 aquatic juvenile (egg, larvae and pupae) and one aerial adult life-stages (Olivier 1971). Previous studies assessing the effect of temperature variation (12-37°C) on chironomid growth patterns concluded that growth was promoted by increasing temperature up to a certain limit (Sankarperumal & Pandian 1991, Stevens 1998, Frouz et al. 2002, Pery & Garric 2006. In contrast, female body size and fecundity decreased with higher temperatures (Frouz et al. 2002, Gong et al. 2002. These studies were all performed with single populations; the additional impact of genetic diversity in connection with the effects of temperature shifts was not investigated. Hence, we tested if the development and reproduction of Chironomus riparius are affected by temperature shifts (± 3°C) and if there is an interaction between these effects and genetic diversity. MATERIALS AND METHODS Test organismsFor the experiments, 6 different Chironomus riparius populations were used (Table 1). One population, with a high inbreeding level (Pop. VI) was obtained from ECT Ecotoxicology, Flörsheim. Five other genetically different populations (Pop. I-V) were derived from different generations of a multi-generation study (under controlled conditions) performed using our inbred inhouse mass culture (GEN-) and a genetically ...

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Section: Resultsmentioning

confidence: 99%

Interaction between genetic diversity and temperature stress on life-cycle parameters and genetic variability in midge Chironomus riparius populations

Vogt

Pupp²,

Nowak³

et al. 2007

Clim. Res.

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“…Later generations showed clear changes, and the susceptibility and GABAR gene expression levels changed in response to the stressor. The phenomenon of increasing adaptation of insect and arthropod populations has been observed after exposure to chemical stressors, including tributyltin (Vogt et al, 2007) and ammonia (Kolarevic et al, 2012) and might be a general response to chemical stress. Results from the present study indicated that spinosad sensitivity of the 3rd instar larvae of P. xylostella declined as the sub-lethal selection cycles increased.…”

Section: Discussionmentioning

confidence: 99%

“…This finding was similar to the changes in LC 25 shown in Table 1. Studies investigating the effects of TBT across multiple generations of Chironomus riparius revealed that TBT increased the tolerance toward the stressor in the TBT-exposed group, but did not alter genetic variability (Vogt et al, 2007). However, exogenous compounds are known to affect gene expression levels, which is associated with the binding element within the promoter that interacts with these exogenous compounds (Tan et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Analysis of persistent changes to γ-aminobutyric acid receptor gene expression in Plutella xylostella subjected to sublethal amounts of spinosad

Zhang

et al. 2016

Genet. Mol. Res.

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ABSTRACT.A multi-generational approach was used to investigate the persistent effects of a sub-lethal dose of spinosad in Plutella xylostella. The susceptibility of various sub-populations of P. xylostella to spinosad and the effects of the insecticide on the gene expression of γ-aminobutyric acid receptor (GABAR) were determined. The results of a leaf dip bioassay showed that the sensitivity of P. xylostella to spinosad decreased across generations. The sub-strains had been previously selected based on a determined LC 25 of spinosad. Considering that GABA-gated chloride channels are the primary targets of spinosad, the cDNA of P. xylostella was used to clone GABARα by using reverse transcription-polymerase chain reaction (RT-PCR). The mature peptide cDNA was 1477-bp long and contained a 1449-bp open reading frame encoding a protein of 483 amino acids. The resulting amino acid sequence was used to generate a neighbor-joining dendrogram, and homology search was conducted using NCBI BLAST. The protein had high similarity with the known GABAR sequence from P. xylostella. Subsequent semi-quantitative RT-PCR and real-time PCR analyses indicated that the GABAR transcript levels in the spinosad-resistant strain (RR, 145.82-fold) and in Sub1 strain (selected with LC 25 spinosad for one generation) were the highest, followed by those in the spinosadsusceptible strain, the Sub10 strain (selected for ten generations), and the Sub5 strain (selected for five generations). This multi-generational study found significant correlations between spinosad susceptibility and GABAR gene expression, providing insights into the long-term effects of sub-lethal insecticide exposure and its potential to lead to the development of insecticide-resistant insect populations.

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“…Other studies (i.e. Branco et al, 2002;Strand and Assmund, 2003;Coelho et al, 2006;Vogt et al, 2007) propose fauna identification and, sometimes, with using sublethal concentrations of Tributyltin (TBT) and invoke biomanipulation (e.g. Crisman and Beaver, 1990;Hansson et al, 1998;Gomez-Ariza et al (1999) to evaluate tolerance dose of biota in order to assist ecotoxicology explanation of urban and peri-urban pollution into riparian systems Pascoe et al (1989).…”

Section: Ecohydrological Dynamics At the Urban Flood Prone Areasmentioning

confidence: 99%

Challenging issues of urban biodiversity related to ecohydrology

Mendiondo

2008

Braz. J. Biol.

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This paper aims to outline challenging issues of urban biodiversity in order to address yardsticks related to ecohydrology, and with a complementary approach to eutrophication impacts. The vision of environmental services, urbanization's consequences and management aspects of water governance are also depicted. Factors of river restoration, environmental tradeoffs and socio-cultural constrains are envisaged through concept questions towards emerging aspects that figure out methodological guides, strategic challenges for stakeholders and inter-disciplinary opportunities. Examples from case studies on restoration and management, from experiences and lessons learned, are enclosed, with brief discussions and literature citation.Keywords: ecohydrology, urban biodiversity, urban waters. Desafios da biodiversidade urbana relacionados com a ecohidrologia ResumoEste artigo aborda desafios sobre a biodiversidade em ambiente urbano com o propósito de apontar uma relação com a ecohidrologia e com especial aproximação aos problemas recorrentes da eutroficação. A visão de serviços ambientais, as conseqüências da urbanização e os aspectos da gestão para uma governança em torno dos recursos hídricos são também apontados no trabalho. Fatores como a recuperação ambiental dos rios, as compensações ambientais e as restrições sócio-culturais são mencionadas usando perguntas conceituais que direcionem aspectos emergentes, no sentido de exemplificar guias metodológicos, desafios estratégicos na negociação junto aos atores e às oportunidades interdisciplinares. Alguns exemplos extraídos a partir de estudos de caso são mostrados, em especial de experiências e lições apreendidas, com discussões e citações da literatura atual do tema.Palavras-chave: ecohidrologia, biodiversidade urbana, águas urbanas.

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Multi-generation studies with Chironomus riparius – Effects of low tributyltin concentrations on life history parameters and genetic diversity

Cited by 73 publications

References 18 publications

Interaction between genetic diversity and temperature stress on life-cycle parameters and genetic variability in midge Chironomus riparius populations

Interaction between genetic diversity and temperature stress on life-cycle parameters and genetic variability in midge Chironomus riparius populations

Analysis of persistent changes to γ-aminobutyric acid receptor gene expression in Plutella xylostella subjected to sublethal amounts of spinosad

Challenging issues of urban biodiversity related to ecohydrology

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