2012
DOI: 10.1111/j.1365-2818.2012.03645.x
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Multi‐image based method to correct vignetting effect in light microscopy images

Abstract: SummaryVignetting is the radial attenuation effect of the image's brightness intensity from the center of the optical axis to the edges. To perform quantitative image analyses it is mandatory to take into account this effect, intrinsic of the acquisition system. Many image processing steps, such as segmentation and object tracking, are strongly affected by vignetting and the effect becomes particularly evident in mosaicing. The most common approach to compensate the attenuation of the image's brightness intens… Show more

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Cited by 49 publications
(36 citation statements)
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“…Further, we compare our two methods with the "Rolling Ball" shading correction algorithm in ImageJ [10], which achieves the best performance amongst several popular retrospective shading correction methods [11]. For WSI image tiles, "Rolling Ball" is used to estimate a shading field of each image tile separately and the common shading is taken as the median of individually estimated shading fields [12]; for an alreadystitched WSI, "Rolling Ball" is used to correct the shading effect of a single image.…”
Section: Experiments and Resultsmentioning
confidence: 99%
“…Further, we compare our two methods with the "Rolling Ball" shading correction algorithm in ImageJ [10], which achieves the best performance amongst several popular retrospective shading correction methods [11]. For WSI image tiles, "Rolling Ball" is used to estimate a shading field of each image tile separately and the common shading is taken as the median of individually estimated shading fields [12]; for an alreadystitched WSI, "Rolling Ball" is used to correct the shading effect of a single image.…”
Section: Experiments and Resultsmentioning
confidence: 99%
“…The first was an inverted Olympus IX51 widefield microscope equipped with an Olympus UPlanFl 10×/0.30na Ph1 objective infinity corrected, while the second was an inverted Zeiss Axiovert 200 widefield microscope equipped with a Zeiss Achroplan 10×/0.25na Ph1 objective infinity corrected. Both microscopes were used in brightfield, and the Köhler illumination alignment [46] was performed in advance.…”
Section: Methodsmentioning
confidence: 99%
“…In practice, in order to accurately register the images of the hemocytometer's grid, we devised and implemented a proper frequency domain approach based on the analysis of the peaks of phase correlation. Furthermore, we developed an effective "blending" technique to make the final mosaic seamless and consistent so as to fix the problem of visible seams in the stitching zones (i.e., colours inhomogeneity and vignetting [37]), which could lead to incorrect cell counting, above all in automatic methods [38]. As a matter of fact, manual counting is a very tedious task and often operators when possible try exploiting, in the very early stage, automatic software to assist them in tagging what, at a first sight, appear to be single cells.…”
Section: Introductionmentioning
confidence: 99%