Multi-immunoreaction-based dual-color capillary electrophoresis for enhanced diagnostic reliability of thyroid gland disease

Woo, Nain; Kim, Su-Kang; Kang, Seong Ho

doi:10.1016/j.chroma.2017.06.044

Cited by 11 publications

(4 citation statements)

References 44 publications

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“…However, it is challenging to distinguish overt hyper/hypothyroidism from subclinical hyper/hypothyroidism or to detect central hypothyroidism by measuring only the TSH value. Woo et al developed a novel method to measure T4, T3, and TSH simultaneously from the same sample using a combination of multi-immunoreaction-based dual-color capillary electrophoresis and a dual-color laser-induced fluorescence technique [ 25 ]. They showed this method had high detection sensitivity and required a short measurement time.…”

Section: Discussionmentioning

confidence: 99%

Determination of Capillary Blood TSH and Free Thyroxine Levels Using Digital Immunoassay

Suzuki,

Takeuchi,

Miyazaki

et al. 2024

Journal of the Endocrine Society

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Background The remote performance of thyroid function blood tests is complicated because it requires blood collection. Objective To compare thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels between capillary and venous blood and assess the adequacy of measuring each value in capillary blood. Methods This prospective intervention study was conducted at Ito Hospital and was based on the clinical research method. The participants were five healthy female volunteers and 50 patients (41 females and 9 males) between the ages of 23 and 81 years. To measure TSH and FT4 levels in capillary and venous blood, a digital immunoassay (d-IA) method capable of measuring trace samples was used. Chemiluminescence measurements were used as controls. Values obtained for each assay system were compared using Spearman's correlation analysis. Capillary blood was collected using an autologous device (TAP II; not approved in Japan). Results Capillary plasma volume obtained using TAP II was 125 µL or more in 26 cases, 25 µL to 124 µL in 24 cases, and less than 25 µL in five cases. Strong correlations were noted in the TSH and FT4 levels between capillary and venous blood, with correlation coefficients of rs = 0.99 and rs = 0.97, respectively. Conclusion Capillary TSH and FT4 levels strongly correlate with venous blood values. Trace samples can be used in high-precision d-IA methods. These results may promote telemedicine in assessing thyroid function.

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Section: Discussionmentioning

confidence: 99%

Determination of Capillary Blood TSH and Free Thyroxine Levels Using Digital Immunoassay

Suzuki,

Takeuchi,

Miyazaki

et al. 2024

Journal of the Endocrine Society

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“…To improve the throughput of CE-LIF, Woo et al built up a CE coupled with dual color LIF (Figure ). This instrumental setup allows the simultaneous detection of thyroxine (T4), triiodothyronine (T3), and thyroid-stimulating hormone (TSH) in a single run of CE for enhancing diagnosis of thyroid gland disease. Interestingly, they also showed that the complex of antibody-T4 could be efficiently separated from the antibody and the antibody–TSH complexes, although the molecular sizes of these species mainly determined by the antibodies are approximately same.…”

Section: Instrumentationmentioning

confidence: 99%

“…1509 , Woo, N., Kim, S. K., Kang, S. H. Multi-immunoreaction-based dual-color capillary electrophoresis for enhanced diagnostic reliability of thyroid gland disease, pp. 153–162 (ref ). Copyright (2017), with permission from Elsevier.…”

Section: Instrumentationmentioning

confidence: 99%

Affinity Interactions by Capillary Electrophoresis: Binding, Separation, and Detection

Zhao

Zhang

et al. 2018

Anal. Chem.

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“…The LOD at S/N = 3 was reported as being in sub μg/L concentrations. Other studies on the detection of thyroxine have used CE‐CL . The assay was based on the reaction between luminol and KMnO 4 in alkaline solution.…”

Section: Applications In Different Clinical Fieldsmentioning

confidence: 99%

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Phillips

2017

Electrophoresis

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Recent advances in CE and microchip-CE in clinical applications: 2014 to mid-2017CE and microchip CE (ME) are powerful tools for the analysis of a number of different analytes and have been applied to a variety of clinical fields and human samples. This review will present an overview of the most recent applications of these techniques to different areas of clinical medicine during the period of 2014 to mid-2017. CE and ME have been applied to clinical chemistry, drug detection and monitoring, hematology, infectious diseases, oncology, endocrinology, neonatology, nephrology, and genetic screening. Samples examined range from serum, plasma, and urine to lest utilized materials such as tears, cerebral spinal fluid, sweat, saliva, condensed breath, single cells, and biopsy tissue. Examples of clinical applications will be given along with the various detection systems employed.

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Multi-immunoreaction-based dual-color capillary electrophoresis for enhanced diagnostic reliability of thyroid gland disease

Cited by 11 publications

References 44 publications

Determination of Capillary Blood TSH and Free Thyroxine Levels Using Digital Immunoassay

Determination of Capillary Blood TSH and Free Thyroxine Levels Using Digital Immunoassay

Affinity Interactions by Capillary Electrophoresis: Binding, Separation, and Detection

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

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