Multi-instrument Evaluation of a Real-time PCR Assay for Identification of Atlantic Salmon: a Case Study on the Use of a Pre-packaged Kit for Rapid Seafood Species Identification

Naaum, Amanda M.; Hellberg, Rosalee S.; Okuma, Tara A.; Hanner, Robert

doi:10.1007/s12161-019-01584-7

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…2453-2468). For example, handheld polymerase chain reaction (PCR) thermocyclers have been commercialized for biosurveillance use, which can connect to mobile devices and generate results in 30-60 min [95] (pp. 2474-2479).…”

Section: Technological Innovations To Improve Canadian Greenhouse Ope...mentioning

confidence: 99%

Canadian Greenhouse Operations and Their Potential to Enhance Domestic Food Security

LaPlante¹,

Andrekovic²,

Young³

et al. 2021

Agronomy

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Food security is a growing societal challenge. The pressure to feed a projected global population of 9.6 billion by 2050 will continue to be limited by decreasing arable land. The recent disruptions in international trade resulting from responses to the COVID-19 pandemic have highlighted the importance of regional self-reliance in food production. While Canada is highly self-reliant in food categories such as meat and dairy, the nation relies heavily on international imports to fulfill fresh vegetable demands. In potential future scenarios where international trade faces disruptions, Canadian food security could be at risk. By providing local sources of fresh foods year-round, the greenhouse vegetable industry holds strong potential to overcome future food supply shortages and could become a critical contributor to self-sustainable food production in Canada. Many challenges, however, surround the Canadian greenhouse industry. Some challenges include the persistence and spread of infectious plant pathogens and forecasted labour shortages. Opportunities to alleviate such challenges include introducing more diverse commodity groups and integrating innovative technologies to accelerate efficiency within the industry. In this commentary, we examine the current state of the Canadian greenhouse industry, explore potential challenges, and highlight opportunities that could promote food security across the nation.

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Section: Technological Innovations To Improve Canadian Greenhouse Ope...mentioning

confidence: 99%

Canadian Greenhouse Operations and Their Potential to Enhance Domestic Food Security

LaPlante¹,

Andrekovic²,

Young³

et al. 2021

Agronomy

Self Cite

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“…However, protein-based analytical methods for species identification are limited because of the proclivity of proteins to denature during heat and high-pressure treatment [16]. DNA-based methods can also be employed to characterize seafood species [17]. Compared to proteins, DNA is more stable during heat and high-pressure processing [18,19].…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR Assay for Simultaneous Identification of Five Types of Tuna (Katsuwonus pelamis, Thunnus alalonga, T. albacares, T. obesus and T. thynnus)

Lee

Suh

Lee

et al. 2022

Foods

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There is a need to identify the species of similar types of fish, especially those that are commercially sold. Particularly, the price of tuna varies depending on its type, which is difficult to determine as they are sold in cut or processed forms. This study developed a multiplex polymerase chain reaction (PCR) assay to identify the five most common tuna species: bigeye, skipjack, Atlantic bluefin, albacore, and yellowfin tunas. Newly designed species-specific primer sets for these five tuna species were created. Subsequently, the amplicon sizes obtained were 270, 238, 200, 178, and 127 base pairs for bigeye, skipjack, Atlantic bluefin, albacore, and yellowfin tunas, respectively. Each primer’s specificity was further tested using 15 other fish species, and no cross-reactivity was observed. To identify multiple targets in a single reaction, multiplex PCR was optimized to increase its resolution and accuracy. The detection levels of the multiplex PCR assay were confirmed to be 1 pg for all the five tunas. Additionally, it was successfully applied to 32 types of commercial tuna products. Therefore, this multiplex PCR assay could be an efficient identification method for various tuna species.

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“…Real-time PCR is a targeted method and cannot be used to simultaneously test for a wide range of species. 4 Besides the molecular methods, traditional methods have also been used to evaluate fish and other seafoods. 5 Despite high accuracies of these methods, they generally need expensive and complicated instruments and time-consuming sample preparation procedures, which prevents them from being used for rapid and high-throughput assessment of the aquatic products.…”

Section: Introductionmentioning

confidence: 99%

Inspecting species and freshness of fish fillets using multimode hyperspectral imaging techniques

Qin

Vasefi²,

Hellberg

et al. 2020

Sensing for Agriculture and Food Quality and Safety XII

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This study developed multimode hyperspectral imaging techniques to detect substitution and mislabeling of fish fillets. Line-scan hyperspectral images were collected from fish fillets in four modes, including reflectance in visible and nearinfrared (VNIR) region, fluorescence by 365 nm UV excitation, reflectance in shortwave infrared (SWIR) region, and Raman by 785 nm laser excitation. Fish fillets of six species (i.e., red snapper, vermilion snapper, Malabar snapper, summer flounder, white bass, and tilapia) were used for species differentiation and frozen-thawed red snapper fillets were used for freshness evaluation. A total of 24 machine learning classifiers were used for fish species and freshness classifications using four types of spectral data in three different subsets (i.e., full spectra, first ten components of principal component analysis, and bands selected by a sequential feature selection method). The highest accuracies were achieved at 100% using full VNIR reflectance spectra for the species classification and 99.9% using full SWIR reflectance spectra for the freshness classification. The VNIR reflectance mode gave an overall best performance for both species and freshness inspection.

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Multi-instrument Evaluation of a Real-time PCR Assay for Identification of Atlantic Salmon: a Case Study on the Use of a Pre-packaged Kit for Rapid Seafood Species Identification

Cited by 10 publications

References 15 publications

Canadian Greenhouse Operations and Their Potential to Enhance Domestic Food Security

Canadian Greenhouse Operations and Their Potential to Enhance Domestic Food Security

Multiplex PCR Assay for Simultaneous Identification of Five Types of Tuna (Katsuwonus pelamis, Thunnus alalonga, T. albacares, T. obesus and T. thynnus)

Inspecting species and freshness of fish fillets using multimode hyperspectral imaging techniques

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