Multi-layer assembly of cellulose nanofibrils in a microfluidic device for the selective capture and release of viable tumor cells from whole blood

Kumar, Tharagan; Soares, Ruben R. G.; Dholey, Leyla Ali; Ramachandraiah, Harisha; Aval, Negar Abbasi; Aljadi, Zenib; Pettersson, Torbjörn; Russom, Aman

doi:10.1039/d0nr05375a

Cited by 13 publications

(13 citation statements)

References 32 publications

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“…Kumar et al [207] Cancer diagnostics are not currently prioritised in resource-limited settings. However, budget-friendly and targeted screening test and diagnostic tools are in great need.…”

Section: Murase Et Al [206]mentioning

confidence: 99%

“…We went through the process of showing how cellulose may be used as a building block for microfluidics chips in the previous part; in this section, we will expand on that to include more complicated design. The layer-by-layer assembly comprises up to 5 layers of CNF that are placed inside a microfluidic channel and are synthesized and characterized with antibodies to trap probable cancer cells [207]. Cellulase enzymes were employed to dissolve the CNF and release the collected cells in 30 min with a negligible influence on cell viability.…”

Section: Advanced Integration Of Cellulose In Microfluidcsmentioning

confidence: 99%

“…This idea might be used to create a colorimetric platform that can quantitatively detect iron levels in water. Reproduced with permissions from[92,160,207,228].…”

mentioning

confidence: 99%

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Cellulose through the Lens of Microfluidics: A Review

Moud

2022

Applied Biosciences

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Cellulose, a linear polysaccharide, is the most common and renewable biopolymer in nature. Because this natural polymer cannot be melted (heated) or dissolved (in typical organic solvents), making complicated structures from it necessitates specialized material processing design. In this review, we looked at the literature to see how cellulose in various shapes and forms has been utilized in conjunction with microfluidic chips, whether as a component of the chips, being processed by a chip, or providing characterization via chips. We utilized more than approximately 250 sources to compile this publication, and we sought to portray cellulose manufacturing utilizing a microfluidic system. The findings reveal that a variety of products, including elongated fibres, microcapsules, core–shell structures and particles, and 3D or 2D structured microfluidics-based devices, may be easily built utilizing the coupled topics of microfluidics and cellulose. This review is intended to provide a concise, visual, yet comprehensive depiction of current research on the topic of cellulose product design and understanding using microfluidics, including, but not limited to, paper-based microfluidics design and implications, and the emulsification/shape formation of cellulose inside the chips.

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Section: Murase Et Al [206]mentioning

confidence: 99%

Section: Advanced Integration Of Cellulose In Microfluidcsmentioning

confidence: 99%

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Cellulose through the Lens of Microfluidics: A Review

Moud

2022

Applied Biosciences

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“…CLSM can also enable researchers to know how thick/thin adsorbed layers are (see Figure a). The layer-by-layer assembly comprised up to 5 layers of CNF that are placed inside a microfluidic channel and were synthesized and characterized with antibodies to trap probable cancer cells . Cellulase enzymes were used to dissolve the CNF and release the collected cells in less than 30 min, with no effect on cell viability, i.e., providing a way to filter cells.…”

Section: Applicationsmentioning

confidence: 99%

Nanocellulose Product Design Aided by Confocal Laser Scanning Microscopy

Moud

2022

ACS Agric. Sci. Technol.

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Nanocellulose is one of the materials with applications in a wide range of technical disciplines, including electronics, oil recovery, robotics, and so on. To understand cellulose material qualities and behavior for product production, cellulose must be characterized separately or as part of a product using a range of approaches. Confocal laser scanning microscopy is one of the techniques that is currently underrepresented in the literature and is suitable to the cellulose domain. Here, we have shown how this characterization tool can uniquely and vastly aid in improving cellulose-based product design. In this brief Review, we looked at the application of confocal laser scanning microscopy in the cellulose domain, with a focus on nanocellulose due to its superior properties; confocal laser scanning microscopy can provide information on intricate structures such as thin layer-by-layer assembly, emulsion, gel stability, and collapse. Additionally, it can provide insight on the extent of enzymatic degradation of cellulose due to morphological changes; furthermore, the FRAP module was introduced briefly, with some of its fundamentals. Later, FRAP applications in primarily suspension and gels (homogeneous and heterogeneous) were introduced to provide examples of possible FRAP usage in cellulose science. In compiling this Review, we have used the most recent publications in the literature.

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“…Carbotrace molecules are fluorescent optotracers which specifically bind to the glycosidic linkages in cellulose and are therefore able to visually map cellulose content using confocal laser microscopy [ 17 , 18 , 19 ]. They have previously been used to identify cellulose in plant cells for mapping cellulosic nanofibrils in microfluidic devices and anatomical mapping in plant cells [ 17 , 18 , 19 ]. However, this paper reports the first use of Carbotrace to analyse defibrillated cellulose obtained from microalgae which were used for the formation of hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Use of Carbotrace 480 as a Probe for Cellulose and Hydrogel Formation from Defibrillated Microalgae

Zitzmann

Ward

Matharu

2022

Gels

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Carbotrace 480 is a commercially available fluorescent optotracer that specifically binds to cellulose’s glycosidic linkages. Herein, the use of Carbotrace 480 is reported as an analytical tool for linking cellulose content to hydrogel formation capability in defibrillated celluloses obtained from proprietary microalgae. Defibrillated celluloses obtained from acid-free hydrothermal microwave processing at low temperature (160 °C) showed poor hydrogel formation attributed to a low cellulose concentration as evidenced through the lack of Carbotrace fluorescence. High temperature (220 °C) processing afforded reasonable gels commensurate with a higher cellulose loading and stronger response to Carbotrace.

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Multi-layer assembly of cellulose nanofibrils in a microfluidic device for the selective capture and release of viable tumor cells from whole blood

Abstract: According to reports by the World Health Organization (WHO), cancer-related deaths reached almost 10 million in 2018. Nearly 65% of these deaths occurred in low- to middle-income countries, a trend...

Cited by 13 publications

References 32 publications

Cellulose through the Lens of Microfluidics: A Review

Cellulose through the Lens of Microfluidics: A Review

Nanocellulose Product Design Aided by Confocal Laser Scanning Microscopy

Use of Carbotrace 480 as a Probe for Cellulose and Hydrogel Formation from Defibrillated Microalgae

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