Multi-layered polydopamine coatings for the immobilization of growth factors onto highly-interconnected and bimodal PCL/HA-based scaffolds

Godoy-Gallardo, María; Portolés‐Gil, Núria; López‐Periago, Ana M.; Domingo, Concepción; Hosta‐Rigau, Leticia

doi:10.1016/j.msec.2020.111245

Cited by 45 publications

(44 citation statements)

References 68 publications

(113 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Assessment of ALP activity was chosen due to the central role of ALP in the initiation of the mineralization process by cell differentiation, displaying the highest activity during the pre-osteoblast differentiation stage [ 12 ]. ALP was evaluated following 14 days of cell culture since it has been previously demonstrated that the ALP activity of hMSCs cultured onto the developed substrates peaked at day 14 [ 16 ]. Interestingly, a similar ALP activity of approximately 15 × 10 −3 pg cells −1 min −1 was observed for all studied ratios including the hMSC monoculture.…”

Section: Resultsmentioning

confidence: 99%

“…The scaffolds were prepared as previously described [ 15 , 16 ]. Briefly, 0.06 g of HA-NPs was added to 2 mL of EL and then magnetically stirred for 30 min followed by sonication for another 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…However, the hydrophobicity of PCL still renders scaffolds with insufficient cell adhesion and proliferation, while HA alone displays limited osteoinductive ability [ 12 ]. Thus, we functionalized the PCL/HA-based scaffolds with a combination of osteoinductive and angiogenic growth factors (GFs) [ 16 ]. Co-delivery of both types of GFs is a powerful approach to promote vascularized bone regeneration [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…While, in our previous work, we identified the conditions promoting the highest incorporation of GFs and osteogenic differentiation of human mesenchymal stem cells (hMSCs) [ 16 ], herein, we further characterize the optimal assembly in terms of osteogenic but also angiogenic potential. Specifically, we fabricate highly interconnected porous PCL/HA composite scaffolds with a bimodal pore size distribution which are subsequently coated by two independent PDA layers loaded with the two GFs ( Scheme 1 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells

Godoy-Gallardo

Portolés‐Gil

López‐Periago

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells

Godoy-Gallardo

Portolés‐Gil

López‐Periago

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…This approach is called layer-by-layer. Notably important to protein delivery, the layer-by-layer method requires facile aqueous baths which potentially preserve soluble protein activity, as the method does not need to use harsh organic solvents [ 140 ]. During tissue regeneration, different GF profiles are present, and the multilayer biotechnology is an open venue that allows for building GF carriers with appropriate delivery kinetics that are able to simulate those GF profiles.…”

Section: Encapsulation Incorporation and Related Delivery Stratementioning

confidence: 99%

Advances in Growth Factor Delivery for Bone Tissue Engineering

Oliveira

Nie

Podstawczyk

et al. 2021

IJMS

136

View full text Add to dashboard Cite

Shortcomings related to the treatment of bone diseases and consequent tissue regeneration such as transplants have been addressed to some extent by tissue engineering and regenerative medicine. Tissue engineering has promoted structures that can simulate the extracellular matrix and are capable of guiding natural bone repair using signaling molecules to promote osteoinduction and angiogenesis essential in the formation of new bone tissues. Although recent studies on developing novel growth factor delivery systems for bone repair have attracted great attention, taking into account the complexity of the extracellular matrix, scaffolding and growth factors should not be explored independently. Consequently, systems that combine both concepts have great potential to promote the effectiveness of bone regeneration methods. In this review, recent developments in bone regeneration that simultaneously consider scaffolding and growth factors are covered in detail. The main emphasis in this overview is on delivery strategies that employ polymer-based scaffolds for spatiotemporal-controlled delivery of both single and multiple growth factors in bone-regeneration approaches. From clinical applications to creating alternative structural materials, bone tissue engineering has been advancing constantly, and it is relevant to regularly update related topics.

show abstract

Improved hemocompatibility by modifying acellular blood vessels with bivalirudin and its biocompatibility evaluation

et al. 2021

J Biomedical Materials Res

View full text Add to dashboard Cite

The incidence rate of cardiovascular diseases is increasing year by year. The demand for coronary artery bypass grafting has been very large. Acellular blood vessels have potential clinical application because of their natural vascular basis, but their biocompatibility and anticoagulant energy need to be improved. We decellularized the abdominal aorta of SD rats, and then modified with bivalirudin via polydopamine. The mechanical properties, blood compatibility, cytocompatibility, immune response, and anticoagulant properties were evaluated, and then the bivalirudin-modified acellular blood vessels were implanted into rats for remodeling evaluation in vivo. The results we got show that the bivalirudin-modified acellular blood vessels showed good cytocompatibility and blood compatibility, and its anti-inflammatory trend was dominant in the immune response. After 3 months of transplantation, the bivalirudinmodified acellular blood vessels did not easily form thrombus. It was not easy to form calcification and could make the host cells grow better. Through vascular stimulation and immunofluorescence test, we found that vascular smooth muscle cells and endothelial cells proliferated well in the bivalirudin group. Bivalirudin-modified acellular blood vessels provided new idea for small diameter tissue engineering blood vessels, and may become a potential clinical substitute for small-diameter vascular grafts.

show abstract

Multi-layered polydopamine coatings for the immobilization of growth factors onto highly-interconnected and bimodal PCL/HA-based scaffolds

Cited by 45 publications

References 68 publications

Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells

Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells

Advances in Growth Factor Delivery for Bone Tissue Engineering

Improved hemocompatibility by modifying acellular blood vessels with bivalirudin and its biocompatibility evaluation

Contact Info

Product

Resources

About