Multi-layered roles of BBX proteins in plant growth and development

Cao, Jing; Yuan, Jiale; Zhang, Yingli; Chen, Chen; Zhang, Beihong; Shi, Xianming; Niu, Rui; Lin, Fang

doi:10.1007/s44154-022-00080-z

Cited by 27 publications

(23 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BBX proteins are known to regulate the transcription of their target genes by directly binding to the upstream regulatory regions (Xu et al, 2016; Song et al, 2020; Yadav et al, 2020a; Cao et al, 2023; Singh and Datta, 2023). Since FT expression is altered in BBX13 -overexpressors, we performed an electrophoretic mobility shift assay (EMSA) to investigate if BBX13 binds to the FT promoter.…”

Section: Resultsmentioning

confidence: 99%

“…The B-box (BBX) family of proteins, which are zinc finger transcription factors, is known to play versatile roles in light-regulated development (Song et al, 2020; Yadav et al, 2020a; Cao et al, 2023). B-box family members contain one or more B-box domains comprised of highly conserved repetitive sequences of cysteine and histidine, with specialized tertiary structures that are stabilized by binding to Zn ions (Klug and Schwabe, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The B-box protein BBX13/COL15 suppresses photoperiodic flowering by attenuating the action of CONSTANS in Arabidopsis

Rahul,

Yadukrishnan,

Sasidharan

et al. 2024

Preprint

View full text Add to dashboard Cite

The optimal timing of transition from vegetative to floral reproductive phase is critical for plant productivity and agricultural yields. Light plays a decisive role in regulating this transition. The B-box (BBX) family of transcription factors regulates several light-mediated developmental processes in plants, including flowering. Here, we identify a previously uncharacterized group II member of the BBX family, BBX13/COL15, as a negative regulator of flowering under long-day conditions. BBX13 is primarily expressed in the leaf vasculature, buds, and flowers. Its spatial expression pattern is similar to the major flowering time regulators CO and FT. bbx13 mutants flower early, while BBX13-overexpressors exhibit delayed flowering under long days. Genetic analyses showed that BBX13 acts upstream to CO and FT in the flowering pathway and negatively regulates their expression. We found that BBX13 physically interacts with CO and inhibits the CO-mediated transcriptional activation of FT. In addition, BBX13 directly binds to the CORE2 motif on the FT promoter, a site where CO also binds. Furthermore, our ChIP data indicates that BBX13 reduces the in vivo binding of CO on the FT promoter. Through luciferase assay, we found that BBX13 inhibits the CO-mediated transcriptional activation of FT. All these data together suggest that BBX13/COL15 represses flowering in Arabidopsis by attenuating CO activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The B-box protein BBX13/COL15 suppresses photoperiodic flowering by attenuating the action of CONSTANS in Arabidopsis

Rahul,

Yadukrishnan,

Sasidharan

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Therefore, it is plausible that interactions with specific protein partners is photoreceptor‐dependent. B‐box domain proteins (BBXs) form another group of pivotal regulatory proteins for photomorphogenic responses (reviewed in Cao et al, 2023). Certain members of the BBX protein family such as BBX31 have been demonstrated to act as positive regulators of UV‐B signalling while they are negative regulators of photomorphogenesis under white light (Yadav et al, 2019).…”

Section: Coordination Of Multiple Signals By Key Signalling Hubsmentioning

confidence: 99%

UV‐B responses in the spotlight: Dynamic photoreceptor interplay and cell‐type specificity

Depaepe

Vanhaelewyn

Straeten

2023

Plant Cell & Environment

View full text Add to dashboard Cite

Plants are constantly exposed to a multitude of external signals, including light. The information contained within the full spectrum of light is perceived by a battery of photoreceptors, each with specific and shared signalling outputs. Recently, it has become clear that UV‐B radiation is a vital component of the electromagnetic spectrum, guiding growth and being crucial for plant fitness. However, given the large overlap between UV‐B specific signalling pathways and other photoreceptors, understanding how plants can distinguish UV‐B specific signals from other light components deserves more scrutiny. With recent evidence, we propose that UV‐B signalling and other light signalling pathways occur within distinct tissues and cell‐types and that the contribution of each pathway depends on the type of response and the developmental stage of the plant. Elucidating the precise site(s) of action of each molecular player within these signalling pathways is key to fully understand how plants are able to orchestrate coordinated responses to light within the whole plant body. Focusing our efforts on the molecular study of light signal interactions to understand plant growth in natural environments in a cell‐type specific manner will be a next step in the field of photobiology.

show abstract

“…For example, KIDARI (KDR) is a small protein with Helix-Loop-Helix (HLH) domain that may act as a repressor of light signaling in Arabidopsis by binding to and inhibiting the activity of a light-controlled bHLH transcription factor (Hong et al, 2013). Another example, BBX31 (length of 121 amino acids) and BBX30 (length of 117 amino acids) allosterically deactivate transcription factors (Song et al, 2020; Cao et al, 2023). Therefore, identifying and characterizing small protein candidates that modulate bHLH transcription factor activity in the Fe signaling pathway can be relevant for better understanding the molecular mechanisms involved in PYE regulation.…”

Section: Introductionmentioning

confidence: 99%

“…Another example, BBX31 (length of 121 amino acids) and BBX30 (length of 117 amino acids) allosterically deactivate transcription factors (Song et al, 2020;Cao et al, 2023). Therefore, identifying and characterizing small protein candidates that modulate bHLH transcription factor activity in the Fe signaling pathway can be relevant for better understanding the molecular mechanisms involved in PYE regulation.…”

Section: Introductionmentioning

confidence: 99%

The small iron-deficiency-induced protein OLIVIA and its relation to the bHLH transcription factor POPEYE

Lichtblau,

Baby,

Khan

et al. 2023

Preprint

View full text Add to dashboard Cite

Iron (Fe) is a crucial micronutrient needed in many metabolic processes. To balance needs and potential toxicity, plants control the amount of Fe they take up and allocate to leaves and seeds during their development. One important regulator of this process is POPEYE (PYE). PYE is a Fe deficiency-induced key bHLH transcription factor (TF) for allocation of internal Fe in plants. In the absence of PYE, there is altered Fe translocation and plants develop a leaf chlorosis.NICOTIANAMINE SYNTHASE4(NAS4),FERRIC-REDUCTION OXIDASE3(FRO3), andZINC-INDUCED FACILITATOR1(ZIF1) genes are expressed at higher level inpye-1indicating that PYE represses these genes. PYE activity is controlled in a yet unknown manner. Here, we show that a small Fe deficiency-induced protein OLIVIA (OLV) can interact with PYE. OLV has a conserved C-terminal motif, that we named TGIYY. Through deletion mapping, we pinpointed that OLV TGIYY and several regions of PYE can be involved in the protein interaction. AnOLVoverexpressing (OX) mutant line exhibited an enhancedNAS4gene expression. This was a mild Fe deficiency response phenotype that was related to PYE function. Leaf rosettes ofolvmutants remained smaller than those of wild type, indicating that OLV promotes plant growth. Taken together, our study identified a small protein OLV as a candidate that may connect aspects of Fe homeostasis with regulation of leaf growth.HighlightsOLIVIA (OLV), a small protein, can interact with the bHLH transcription factor POPEYE (PYE)OLV has a conserved motif, named TGIYY, that can be involved in protein interaction with PYEOLV mutant plants have mild phenotypes related with PYE andNAS4regulation.OLV promotes rosette growth.

show abstract

Multi-layered roles of BBX proteins in plant growth and development

Cited by 27 publications

References 126 publications

The B-box protein BBX13/COL15 suppresses photoperiodic flowering by attenuating the action of CONSTANS in Arabidopsis

The B-box protein BBX13/COL15 suppresses photoperiodic flowering by attenuating the action of CONSTANS in Arabidopsis

UV‐B responses in the spotlight: Dynamic photoreceptor interplay and cell‐type specificity

The small iron-deficiency-induced protein OLIVIA and its relation to the bHLH transcription factor POPEYE

Contact Info

Product

Resources

About