Multi-layered stimuli responsive DNA micelles for the stepwise controlled release of small molecules

Abstract

DNA functionalized Multi layered Surface Crosslinked Micelles (mlSCMs) can compartmentalize two small molecule cargo in distinct layers. In response to the appropriate trigger, mlSCMs can release cargo for chemical and biochemical applications.

Controlled Release of a Dual Zinc‐Sensing and Gene Regulating Small Molecule from DNA Micelles

“…B. Synthesis of 1.6 % TPEN loaded NANs : A previously reported protocol was followed for the synthesis of NANs with cargo encapsulation [25,26,45] . 1.1 mg of surfactant (trialkyl modified) was dissolved in 345 μL of Millipore water (10 mM).…”

“…A previously reported protocol was followed for the synthesis of NANs with cargo encapsulation. [25,26,45] 1.1 mg of surfactant (trialkyl modified) was dissolved in 345 μL of Millipore water (10 mM). 6.25 μL of 10 mM TPEN (stock solution in DMSO) was added to the surfactant solution and sonicated for 30 min at room temperature.…”

DNA-functionalized metal or metal-containing nanoparticles for biological applications

“…Notably, spherical nucleic acid conjugates exhibit different properties from those of the Au core and DNA molecules from which they are derived. Recently, with the development of DNA technologies and NP construction methods, various types of DNA-functionalized NPs have been studied, with a diverse range of applications in nanomaterial assembly, 18,19 biosensor development, [20][21][22] and drug delivery. [23][24][25] Among these, the DNA functionalization of metal or metal-containing nanoparticles (M/MC NPs) has gained increasing attention.…”