Multi-level chemical characterization and anti-inflammatory activity evaluation of the polysaccharides from Prunella vulgaris

Sun, He; Lou, Jia; Chen, Bo-Xue; Huang, Jia-Qi; Wang, Qi-Long; Song, Shao-Fei; Jia, Zi-Yue; Miao, Rong; Wang, Shi-Yu; Li, Xue; Yang, Wen-Zhi

doi:10.1016/j.fitote.2024.105841

Cited by 3 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the field of food science, polysaccharides' texture and mouthfeel can be impacted by protuberances. The hydroxyl groups in the polysaccharides' skeletal form provide hydrogen bonds to form the aggregation of the fractions; this is due to high intermolecular and intramolecular interaction between the residues [36]. Overall, acidic polysaccharide fractions can be subjected to AFM analysis to learn more about their physical characteristics and behavior.…”

Section: Afm Analysismentioning

confidence: 99%

Chemical Characterization Analysis, Antioxidants, and Anti-Diabetic Activity of Two Novel Acidic Water-Soluble Polysaccharides Isolated from Baobab Fruits

Song,

Abubaker,

Akhtar

et al. 2024

Foods

View full text Add to dashboard Cite

This study explores the isolation and characterization of two acidic polysaccharides from baobab (Adansonia digitata) fruits, named ADPs40-F3 and ADPs60-F3; the two types of acidic polysaccharides exhibited high sugar content and chemical structural features characterized by O–H, C–H, carbonyl C=O, and COOH carboxyl functional groups. The two fractions showed molecular weights of 1.66 × 105 and 9.59 × 104 Da. ADPs40-F3 residues consist of arabinose (2.80%), galactose (0.91%), glucose (3.60%), xylose (34.70%), and galacturonic acid (58.10%). On the other hand, ADPs60-F3 is composed of rhamnose (1.50%), arabinose (5.50%), galactose (2.50%), glucose (3.10%), xylose (26.00%), and galacturonic acid (61.40%). Furthermore, NMR analysis showed that the main acidic structures of ADPs40-F3 and ADPs60-F3 are formed by 4,6)-α-d-GalpA-(1→, →4)-β-d-Xylf-(1→, →4,6)-β-d-Glcp-(1→, →5)-α-L-Araf-(1→, →4,6)-α-d-Galp-(1→ residues and 4)-α-d-GalpA-(1→, →4)-β-d-Xylf-(1→, →6)-β-d-Glcp-(1→, →5)-α-l-Araf-(1→ 4,6)-α-d-Galp-(4,6→, →2)-α-Rhap- residues, respectively, based on the observed signals. Antioxidant assays against DPPH, ABTS+, and FRAP revealed significant antioxidant activities for ADPs40-F3 and ADPs60-F3, comparable to ascorbic acid (VC). Additionally, both polysaccharides exhibited a dose-dependent inhibition of α-glucosidase and α-amylase activities, suggesting potential anti-diabetic properties. In vivo evaluation demonstrated that ADPs60-F3 significantly reduced blood glucose levels, indicating promising therapeutic effects. These findings underscore the potential utility of baobab fruit polysaccharides as natural antioxidants and anti-diabetic agents.

show abstract

Section: Afm Analysismentioning

confidence: 99%

Chemical Characterization Analysis, Antioxidants, and Anti-Diabetic Activity of Two Novel Acidic Water-Soluble Polysaccharides Isolated from Baobab Fruits

Song,

Abubaker,

Akhtar

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

Structural Characterization and Anti‐inflammatory Activities of a Purified Polysaccharide from Veronica persica Poir.

Song,

Gu,

Kong

2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

In this manuscript, the polysaccharide (VPP‐I) from Veronica persica Poir., was characterized in detail by high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FT‐IR) and nuclear magnetic resonance spectroscopy (NMR). In addition, lipopolysaccharide (LPS) induced inflammation model of RAW264.7 cells was used to evaluate the in vitro anti‐inflammatory activity of VPP‐I. The results showed that the relative molecular weight of VPP‐I was 2.355 KDa, which was mainly composed of mannose (Man), glucose (Glc) and galactose (Gal) in a ratio of 1:32.46:28.76. Moreover, the VPP‐I contained sugar alcohol derivatives of T‐D‐Glcp(1→, →4)‐D‐Galp(1→, →3,6)‐D‐Manp(1→, →4)‐D‐Glcp(1→, →6)‐D‐Galp(1→ and →6)‐D‐Glcp(1→. In vitro anti‐inflammatory results showed that VPP‐I could inhibit the secretion of IL‐β, IL‐6 and TNF‐α in RAW264.7 cells induced by LPS. Moreover, compared to the LPS group, the mRNA expression levels of iNOS, COX‐2, IL‐β, IL‐6 and TNF‐α produced by RAW264.7 were significantly decreased after treatment with VPP‐I (P<0.05). In addition, VPP‐I could increase the SOD and GSH‐Px enzymes activity and decrease the content of MDA in LPS‐induced RAW264.7 cells (P<0.05). In summary, this paper laid theoretical foundation for the application of Veronica persica Poir. in the field of medicine.

show abstract

Characterization and identification of the wide-polarity multicomponents from Prunella vulgaris by offline two-dimensional liquid chromatography and hydrophilic interaction chromatography coupled to ion mobility-quadrupole time-of-flight mass spectrometry

Sun,

Wang,

Huang

et al. 2024

Journal of Chromatography A

View full text Add to dashboard Cite

Multi-level chemical characterization and anti-inflammatory activity evaluation of the polysaccharides from Prunella vulgaris

Cited by 3 publications

References 30 publications

Chemical Characterization Analysis, Antioxidants, and Anti-Diabetic Activity of Two Novel Acidic Water-Soluble Polysaccharides Isolated from Baobab Fruits

Chemical Characterization Analysis, Antioxidants, and Anti-Diabetic Activity of Two Novel Acidic Water-Soluble Polysaccharides Isolated from Baobab Fruits

Structural Characterization and Anti‐inflammatory Activities of a Purified Polysaccharide from Veronica persica Poir.

Characterization and identification of the wide-polarity multicomponents from Prunella vulgaris by offline two-dimensional liquid chromatography and hydrophilic interaction chromatography coupled to ion mobility-quadrupole time-of-flight mass spectrometry

Contact Info

Product

Resources

About