Multi-locus Analyses Indicate that <i>Melastoma dendrisetosum</i>, an Endemic and Endangered Shrub in Hainan, is a Distinct Species

Huang, Guilian; Liu, Ying; Wu, Wei; Ng, Wei Lun; Ning, Zu-Lin; Zhang, Rongjing; Fan, Qingxia; Zhou, Renchao

doi:10.1600/036364418x697120

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Total DNA was extracted from dried leaf tissue using the modified CTAB procedure (Doyle and Doyle 1987). Six low-copy nuclear genes ( cam , chi , gapC , gbss , tpi and vr ) were amplified and sequenced using primers published in previous studies of Melastoma (Dai et al 2012; Chao et al 2014; Zhou et al 2017; Huang et al 2018). For nrITS and chloroplast intergenic spacer trnL – trnF , universal primers were used (White et al 1990; Taberlet et al 1991).…”

Section: Methodsmentioning

confidence: 99%

“…Species delimitation in Melastoma is also problematic. Multiple names have often been applied for the same taxa across different works (Keng and Li 1977; Chen 1984; Huang and Huang 1993; Meyer 2001; Yang and Liu 2002; Chen and Renner 2007; Dai et al 2012; Chao et al 2014; Huang et al 2018). Regretfully, these issues have not been fully addressed using an integrative approach.…”

Section: Introductionmentioning

confidence: 99%

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The specific status of Melastoma kudoi (Melastomataceae, Melastomeae)

et al. 2019

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Background Melastoma has undergone rapid species radiation during the last one million years, and circumscription of some species in the genus has remained controversial. Melastoma kudoi , an erect species narrowly endemic to central Taiwan was previously treated as a synonym of M. intermedium , a semicreeping hybrid between the erect species M. candidum and the creeping M. dodecandrum , making its identity questionable. We addressed this question based on molecular and morphological data. Results Phylogenetic analyses based on nrITS sequence data revealed that M. kudoi is most closely related to M . dodecandrum . Further analyses of six nuclear genes ( cam, chi, gapC, gbss, tpi and vr ) and two chloroplast markers ( trnL – trnF and psbA ) showed that M. kudoi is well diverged from its close relatives. Morphologically, it is also easily distinguished from related species by its erect habit, center-positioned stigma, and spreading, basally enlarged hairs on the hypanthium. Conclusions Both molecular phylogenetic and morphological data suggest that M. kudoi is well separated from M. intermedium , M . dodecandrum , and O . scaberrima , and should be treated as a distinct species. Taxonomic treatment and detailed description of M. kudoi are provided. Electronic supplementary material The online version of this article (10.1186/s40529-019-0253-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The specific status of Melastoma kudoi (Melastomataceae, Melastomeae)

et al. 2019

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“…We used CTAB method (Doyle & Doyle, 1987) to extract DNA from dried leaves. Six nuclear genes adopted in previous studies Dai et al, 2012;Huang et al, 2017;Zou et al, 2017) were tested in the three taxa and three nuclear genes (gbss, vr, and tpi) showed successful PCR amplification in all three taxa. Gbss, tpi, and vr encode granule-bound starch synthase, triose phosphate isomerase, and vacuolar invertase, respectively.…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

Molecular identification of natural hybridization betweenMelastoma malabathricumandMelastoama beccarianumin Sarawak, Malaysia

Zou

Tan³

et al. 2019

Ecology and Evolution

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Hybridization is very common in flowering plants and it plays a significant role in plant evolution and adaptation. Melastoma L. (Melastomataceae) comprises about 80–90 species in tropical Asia and Oceania, among which 41 species occur in Borneo. Natural hybridization is frequently reported in Melastoma in China, but so far there have been no confirmed cases of hybridization in Southeast Asia (including Borneo), where most species occur. Here, we identified a case of natural hybridization between Melastoma malabathricum L. and Melastoma beccarianum Cogn. in Sarawak, Malaysia, by using sequence data of three nuclear genes and one chloroplast intergenic spacer. Melastoma malabathricum is the most widespread species of this genus, occurring in almost the whole range of this genus, while M. beccarianum is a local species endemic to northern Borneo. Our results showed that natural hybridization and introgression occur between M. malabathricum and M. beccarianum, and the introgression was asymmetrical, mainly from M. malabathricum to M. beccarianum. As adaptive traits can be transferred by introgression, our study suggests that natural hybridization should be a significant mechanism for the evolution and adaptation of Melastoma in Southeast Asia. However, introgression from the common species M. malabathricum to the relatively rare species M. beccarianum may cause the decline of M. beccarianum, incurring conservation concern. With a large number of species of Melastoma and almost year‐around flowering in Southeast Asia, more cases of natural hybridization are expected to be found and identified in near future.

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“…In China, nine Melastoma species were recorded in the south of the Yangtze River by Chen (1984), yet only five of them were recognized in more recent publications (Meyer 2001;Chen and Renner 2007). This revised treatment has incorporated M. affine, M. normale and M. candidum into M. malabathricum, but this has not been accepted by several plant taxonomists (Huang et al 2018).…”

Section: Introductionmentioning

confidence: 99%