Multi-locus variable number tandem repeat analysis of Vibrio cholerae isolates from 2012 to 2013 cholera outbreaks in Iran

Ranjbar, Reza; Sadeghy, J.; Moghadam, Mohammad Shokri; Bakhshi, Bita

doi:10.1016/j.micpath.2016.05.023

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…The MLVA is a method for typing bacteria, which has been applied to several different species of bacteria. This method has appropriate differentiation power, is low cost, does not take long to run, and can be performed for a large number of samples (18)(19)(20)(21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus epidermidis, Clonality and Accessory Gene Regulator Diversity in Clinical Isolates

Peerayeh

Behmanesh

Moghadas

2018

Arch Clin Infect Dis

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Background: Quorum-sensing systems are considered as important mechanisms for pathogenesis and bacterial communication. The accessory gene regulator (agr) is one of the quorum-sensing systems in staphylococci, which is generally conserved. It is believed that there is a correlation between agr groups and infection. Multiple-locus VNTR analysis is a method for bacterial typing, previously applied for several different species of bacteria. This study aimed at determining the diversity of Staphylococcus epidermidis accessory gene regulator and clonality in clinical isolates from intensive care unit patients, Tehran, Iran. Methods: A total of 59 Staphylococcus epidermidis isolates were obtained from intensive care unit patients. The MLVA was performed for S. epidermidis isolates, using seven VNTR loci, including SE2395, SE0331, SE 828, SE1632, SE0175, Se2, and Se4. Specific primers were used for agr diversity determination. Results: The agr type I was observed in 29 (49%), while each of the agr type II and agr type III were observed in 10 (17%). Furthermore, 10 (17%) isolates were untypeable with using primers. In total, 49 MLVA genotypes were discriminated. Isolates were classified to six clonal complexes. Of the 59 isolate, 33 were included in clonal complex 1 (CC1), the largest of which harbored 15 (45.4%) agr type I. Conclusions: Agr type I was observed in the majority of the isolates. The MLVA results of this study suggest that there was a clone with 33 samples, comprised of 56% of isolates; smaller clones each comprised of two to seven isolates, and four isolates in the form of singleton. It seems that big clone isolates were settled in the intensive care unit (ICU), and singleton isolates entered the ward by visitors or medical personnel and caused infection.

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Section: Introductionmentioning

confidence: 99%

Staphylococcus epidermidis, Clonality and Accessory Gene Regulator Diversity in Clinical Isolates

Peerayeh

Behmanesh

Moghadas

2018

Arch Clin Infect Dis

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“…Other pathogenic species of Vibrio including V. cholerae are among most common food and water borne bacterial pathogens in our country (39-43). Similar studies are suggested to determine antibacterial effects of lavender essence on endemic pathogenic V. cholera in the future.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Lavandula Stoechas on Toxigenesis and the Growth of Vibrio Parahaemolyticus

et al. 2018

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Methods: lavender essence was prepared and its components were identified using GCMS. Determining minimum interceptorgrowth of Vibrio parahaemolyticus was assessed in test tubes containing BHI. Thermal resistant hemolysin was measured by Kap-RPLAkit. Growth diagram was prepared after determining toxin formation titration of the bacterium during 0, 2, 3, 4, 6, 8 and 24 hours. Results: Cineol, Borneol, Camphor, LinaloolL and Alpha-pinen had the highest concentrations in the essence, respectively. Results of minimum intercepter concentration of lavender (0, 0.005, 0.015, 0.03 and 0.045 percent) on Vibrio parahaemolyticus showed that 0.03% and higher concentrations had the ability to prevent growth and toxin formation of Vibrio parahaemolyticus. In addition, the effect of different concentrations of essence on toxin titration of bacterium showed no toxin at concentrations of 0.030 and 0.045. Conclusion: lavender essence was able to prevent the growth and toxin formation of Vibrio parahaemolyticus.

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“…при молекулярном типировании методом MLVA штаммов V. cholerae O1, обусловивших вспышки холеры в иране (2012-2013 гг. ), среди 30 изученных штаммов один штамм принадлежал к классическому биовару [46]. вспышки и спорадические случаи хо-леры бенгал периодически имели место в индии и китае [17, 40,41].…”

Section: Tcpaunclassified

Cholera: Assessment of Epidemiological Situation on Cholera around the World and in Russia in 2008–2017. Forecast for 2018

Москвитина¹,

Тюленева²,

Кругликов³

et al. 2018

Problemy Osobo Opasnykh Infektsii

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36Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections] холера продолжает оставаться значимой про-блемой для здравоохранения многих стран мира, что, в свою очередь, определяет организацию и проведе-ние, а также выработку приоритетных направлений профилактической деятельности, предусмотренных на международном, региональном и национальном уровнях [3, 38,45]. при этом мониторинг динамики заболеваемости холерой, а также контаминации хо-лерными вибрионами о1 и о139 серогрупп поверх-ностных водоемов и других объектов окружающей среды, составляет основу эффективных, целенаправ-ленных, превентивных и контрольных противохо-лерных мероприятий.использованы сведения проблемно-ориенти-рованных баз данных «холера эль-тор. эпидемио-логический анализ заболеваемости в мире», «холера Пробл. особо опасных инф. 2018; 1:36-43 , several strains of the same or closely related genotypes in some regions were isolated every year. For the first time ever the algorithm for risk of epidemic process activation assessment in case of cholera in Russia, taking into account the various factors (conditions), their gradation and expert scores, has been developed for the prediction. The forecast for cholera at the global level and in Russia for 2018 is unfavorable.

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Multi-locus variable number tandem repeat analysis of Vibrio cholerae isolates from 2012 to 2013 cholera outbreaks in Iran

Cited by 9 publications

References 24 publications

Staphylococcus epidermidis, Clonality and Accessory Gene Regulator Diversity in Clinical Isolates

Staphylococcus epidermidis, Clonality and Accessory Gene Regulator Diversity in Clinical Isolates

The Effect of Lavandula Stoechas on Toxigenesis and the Growth of Vibrio Parahaemolyticus

Cholera: Assessment of Epidemiological Situation on Cholera around the World and in Russia in 2008–2017. Forecast for 2018

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