2022
DOI: 10.1101/2022.04.17.488590
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Multi-marker DNA metabarcoding detects suites of environmental gradients from an urban harbour

Abstract: There is increasing need for biodiversity monitoring, especially in places where potential anthropogenic disturbance may significantly impact ecosystem health. We employed a combination of traditional morphological and bulk macroinvertebrate metabarcoding analyses to benthic samples collected from Toronto Harbour (Ontario, Canada) to compare taxonomic and functional diversity of macroinvertebrates and their responses to environmental gradients. At the species rank, sites assessed using COI metabarcoding showed… Show more

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Cited by 1 publication
(2 citation statements)
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“…As a part of collaborative work with Environment and Climate Change Canada, MetaWorks was used to assess macroinvertebrate and (non-macroinvertebrate) eukaryote taxa in an urban harbour using COI and 18S rRNA [56]. Using the MetaWorks pipeline, COI metabarcodes were identified down to species rank with 99% accuracy and 18S metabarcodes were identified to genus rank with 80% accuracy using a custom-trained classifier based on the SILVA 18S release 138 [38].…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…As a part of collaborative work with Environment and Climate Change Canada, MetaWorks was used to assess macroinvertebrate and (non-macroinvertebrate) eukaryote taxa in an urban harbour using COI and 18S rRNA [56]. Using the MetaWorks pipeline, COI metabarcodes were identified down to species rank with 99% accuracy and 18S metabarcodes were identified to genus rank with 80% accuracy using a custom-trained classifier based on the SILVA 18S release 138 [38].…”
Section: Resultsmentioning
confidence: 99%
“…Canada, MetaWorks was used to assess macroinvertebrate and (nonmacroinvertebrate) eukaryote taxa in an urban harbour using COI and 18S rRNA [56].…”
Section: Use Case 3: As a Part Of Collaborative Work With Environment...mentioning
confidence: 99%