Multi-metal resistance and potential of Alcaligenes sp. MMA for the removal of heavy metals

Abstract

Pollution of water bodies is increasing because of toxic metals. In view of this, the present study examines the removal efficiency of heavy metals Cu 2+ , Cd 2+ , Cr 6+ , Ni 2+ , and Zn 2+ by a bacterial strain isolated from River Yamuna. Initially, three strains were isolated, and further examined for the maximum tolerance index. Among the three isolates, strain MMA showed the highest tolerance to the heavy metals and was selected. The biochemical characterization and phylogenetic analysis based on 16S rRNA gene sequencing showed that the strain belonged to the genus Alcaligenes. Metal removal by the strain was observed at 20 mg/L in both individual and multi-metal experimental setup. The highest metal removal within 72 h was observed for Cu 2+ (88.45%) followed by Ni 2+ (82.45%), Zn 2+ (69.99%), Cd 2+ (63.04%), and least for Cr 6+ (48.93%). Bacterial biosorption studies were done using SEM-EDX and FT-IR. Along with the metal removal, the heavy metal assessment of the River was also done for the year September 2017-August 2018, representing the post-monsoon, monsoon, and pre-monsoon. Cu 2+ , Cd 2+ , Cr 6+ , and Zn 2+ were detected in the water sample due to the uncontrolled discharge of effluents into the river.

Comparative Genomics Reveals Novel Species and Insights into the Biotechnological Potential, Virulence, and Resistance of Alcaligenes

“…Alcaligenes species have the potential to promote biofertilization, bioremediation, and heavy metal tolerance [ 8 , 9 , 43 , 44 , 45 , 46 , 47 ]. We investigated the potential of Alcaligenes species for these properties by integrating data from PGPTs available in the PlaBAse database.…”

Metal tolerance and biosorption capacities of bacterial strains isolated from an urban watershed

“…R3, and 1,118, 2,986, and 2,997 cm −1 for Klebsiella sp. R19 can be attributed to the presence of phosphate and amino groups and are linked to the presence of phosphate and amino groups, playing a pivotal role in the biosorption of metals ( Sodhi et al, 2020 ).…”

Perspective on the heavy metal pollution and recent remediation strategies

“…MMA Bacteria Cu 2+ , Cd 2+ , Cr 6+ , Ni 2+, and Zn 2+ At temperature 28℃ and pH 7, 88.45% Cu 2+ , 53.04% Cd 2+ , 48,93% Cu 2+ , Cd 2+ , Cr 6+ , Ni 2+, and Zn 2+ at 20 mg/L of concentration. ( Sodhi et al., 2020 ) Bacillus sp. strain Bacteria Cd 2+ HM was removed at temperatures 30℃ and 200 rpm and with total metal removal of 90.14% ( Miretzky et al., 2004 ) Penicillium notatum Fungi Cd 2+ 77.67% biosorption in 28 days at temperature 28℃ and pH 7 (Mishra et al., 2008) Aspergillus niger Fungi Ni 2+ 81.07% biosorption in 28 days at temperature 28℃ and pH 7 ( Oyewole et al., 2019 ) Pistia stratiotes Plant root and leaves Cr 6+ 100% Cr 6+ removal in 72 h at an initial concentration of 5 mg/L ( Koul and Taak, 2018 ) Pistia stratiotes Plant root and leaves Cr 6+ 97.3%, 72.2%, and 73.5% Cr 6+ removed at 1,2, and 4 mg/L of initial metal solution in 190 h. ( Shrestha et al., 2021 ) Eichhornia crassipes Plant root and leaves Cr 6+ 84% removal of Cr 6+ achieved in 11 days (Sodhi et al., 2021) …”