Multi-mycotoxins analysis in raw milk by ultra high performance liquid chromatography coupled to quadrupole orbitrap mass spectrometry

Mao, Jianfei; Zheng, Nan; Fang, Wen; Guo, Ling-An; Fu, Chaomei; Ouyang, Huaxue; Zhong, Lingli; Wang, Jiaqi; Lei, Shaorong

doi:10.1016/j.foodcont.2017.08.009

Cited by 33 publications

(22 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The LODs and LOQs that were obtained showed the suitability of the developed methods for the determination of trace amounts of the selected mycotoxins in the milk samples. In addition, the LOQs that were obtained here were up to 10 times lower than those that were reported by other authors using triple quadrupole mass analyzer, and were similar than those that were reported in a recent studies employing HRMS [21] (Table 3). Nevertheless, this last study used immunoaffinity columns, which significantly increase thed sample preparation procedure in terms of time and cost, compared with the inexpensive QuEChERS.…”

Section: Limits Of Detection and Limits Of Quantificationsupporting

confidence: 86%

“…Table 3 summarizes the available studies focused on the occurrence of AFB1 and its metabolite AFM1 in milk samples. The occurrence of AFB1 was reported in 1.2% (n = 80) [32], 1.4% (n = 290) [33], 12.4% (n = 250) [21], and 17.5% (n = 40) [9] of Egyptian, Mexican, Chinese, and Brazilian milk samples, respectively, in which were found concentrations of up to 3.54 µg/L. These results were of particular concern because of the lack of regulation and considering that AFB1 was a recognized human carcinogen [3].…”

Section: Application To Samplesmentioning

confidence: 99%

“…In this sense, not much literature has considered evaluating the occurrence of AFB1 in milk, despite the fact that it is a carcinogenic compound to humans [18][19][20]. For instance, a recent study reported an incidence of AFB1 and AFM1 in 12.4% and 98.8%, respectively, of Chinese milk samples (n = 250) [21]. Therefore, humans are potentially exposed to these toxic metabolites and it becomes necessary to take a vigilant attitude in order to minimize the human intake of aflatoxins.…”

Section: Introductionmentioning

confidence: 99%

“…In this sense, liquid chromatography mass spectrometry (LC-MS) methods, for multi-mycotoxin analysis in milk, have been reported in the literature [6,22,23]. In recent years, there has been increasing interest in evaluating the capability of the high-resolution mass spectrometry (HRMS) for multi-mycotoxin analysis because it provides not only a high resolution and accuracy mass results, but also a high sensibility and complementary structural information when compared with other MS detectors [21,24].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous Determination of AFB1 and AFM1 in Milk Samples by Ultra High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap Mass Spectrometry

et al. 2018

View full text Add to dashboard Cite

Abstract:Milk is the world's most consumed beverage, not counting water. Even though investigations on milk aflatoxin (AF) M1 contamination are regularly conducted, there is limited information on the contamination of milk with its parent compound, AFB1. Hence, the aim of this study was to develop a quick, easy, cheap, effective, rugged, and safe (QuEChERS)-based method for the simultaneous analysis of AFB1 and AFM1 in milk, using ultrahigh performance liquid chromatography coupled with high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). The recoveries were in a range of 75-96% at 0.005, 0.01, and 0.05 µg/L spiking levels, with repeatability and reproducibility results expressed as relative standard deviations (RSDs) lower than 7% and 16%, respectively. The limits of detection (LODs) and quantification (LOQs) were 0.001 and 0.002 µg/L for AFM1 and AFB1, respectively. The LODs and LOQs that were obtained showed the suitability of the developed method for the determination of trace amounts of the selected mycotoxins in milk samples, and were up to ten times lower than those that had been reported in previous works using triple quadrupole mass analyzers. The matrix effect was evaluated and matrix-matched calibrations were used for quantification. The validated method was applied to 40 Italian milk samples. Neither AFB1 nor AFM1 were found above the LOD in any of the analyzed samples.

show abstract

Section: Limits Of Detection and Limits Of Quantificationsupporting

confidence: 86%

Section: Application To Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous Determination of AFB1 and AFM1 in Milk Samples by Ultra High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap Mass Spectrometry

et al. 2018

View full text Add to dashboard Cite

show abstract

“…La flora del rumen es considerada una defensa contra las micotoxinas por el hecho de que algunas de ellas (ocratoxina A, deoxinivalenol, aflatoxina B1 y zearalenona) son allí metabolizadas a compuestos de menor toxicidad, aunque otras micotoxinas como patulina o las fumonisinas atraviesan la barrera del rumen sin sufrir cambios, aunque esto no implica su posterior transferencia a la leche [89]. Aunque la presencia en leche de distintas clases micotoxinas como FB1, ZEA, OTA, AFB1 y sus metabolitos se ha reportado con variados niveles de ocurrencia [89][90][91], la micotoxina de mayor importancia en leche ha sido siempre la aflatoxina M1 [92]. Este compuesto es el derivado hidroxilado de la aflatoxina B1 que comúnmente contamina las materias primas que se utilizan en la alimentación del ganado, y tal es su importancia que es la única micotoxina para la cual existen límites máximos permitidos en leche.…”

Section: La Leche Como Vector De Contaminantesunclassified

National short-term dietary exposure assessment of a selected group of pesticides in Argentina

Maggioni

Signorini

Michlig

et al. 2018

Journal of Environmental Science and Health, Part B

View full text Add to dashboard Cite

An evaluation of acute dietary exposure to pesticide residues, applying deterministic and stochastic methods, was performed for a selected group of pesticides in two representative age groups from Argentina. Thus, 28 active ingredients (a.i.) and 75 food items were evaluated for the group of 2-5-year-old children, while 9 a.i. and 59 food items were considered for the 10-49-year-old women group. A deterministic assessment was conducting following the Food and Agriculture Organization (FAO) and World Health Organization (WHO) procedure but using the national maximum residue limits (MRLs) as pesticide residue concentration data, while in the stochastic approach, a theoretical distribution modeled with the available information was used. Food consumption data were obtained from the 2004-2005 comprehensive national nutrition and health survey. The risk was estimated by comparing the short-term dietary exposure with the acute reference dose (ARfD) values for each pesticide-food combination evaluated. In the deterministic assessment, 173 (39.1%) and 40 (31.3%) combinations exceeded the ARfD thresholds for the 2-5-year-old children and 10-49-year-old women groups, respectively. This conservative study generated relevant information as a first stage of acute dietary risk assessment in Argentina.

show abstract

Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC–MS/MS

Yang

Luo

Mu³

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

An liquid chromatography–tandem mass spectrometry method coupled with a stable isotope dilution assay was established for the simultaneous detection of 17 mycotoxins and their derivatives (aflatoxins B1, B2, G1, G2, M1, and M2; fumonisins B1 and B2; ochratoxin A; zearalenone; zearalanone; α‐zearalanol; α‐zearalenol; T‐2 toxin; deoxynivalenol; deepoxy‐deoxynivalenol; and sterigmatocystin) in milk and dairy products. The mycotoxins were extracted with acidified acetonitrile and the lipids were removed using a Captiva EMR‐lipid column. The average recoveries of the target compounds from samples spiked at three different concentrations were 67–102%, and the relative standard deviations of the peak areas were less than 10%. Limits of quantification (S/N = 10) of 0.004–1.25 μg/kg were achieved, which are significantly lower than the maximum levels allowed in various countries and regions for each regulated mycotoxin. Milk and yogurt products from local markets and e‐commercial platforms were analyzed using the optimized method. The screening showed that aflatoxin M1, deoxynivalenol, fumonisins B1 and B2, and zearalenone could be found in milk and yogurt products, especially those products also containing grains or jujube ingredients, indicating that there is a risk of mycotoxins in dairy products.

show abstract

Multi-mycotoxins analysis in raw milk by ultra high performance liquid chromatography coupled to quadrupole orbitrap mass spectrometry

Cited by 33 publications

References 46 publications

Simultaneous Determination of AFB1 and AFM1 in Milk Samples by Ultra High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap Mass Spectrometry

Simultaneous Determination of AFB1 and AFM1 in Milk Samples by Ultra High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap Mass Spectrometry

National short-term dietary exposure assessment of a selected group of pesticides in Argentina

Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC–MS/MS

Contact Info

Product

Resources

About