2020
DOI: 10.1038/s41598-020-78351-w
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Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain

Abstract: Osmoregulation and osmoconformation are two mechanisms through which aquatic animals adapt to salinity fluctuations. The euryhaline crab Scylla paramamosain, being both an osmoconformer and osmoregulator, is an excellent model organism to investigate salinity adaptation mechanisms in brachyurans. In the present study, we used transcriptomic and proteomic approaches to investigate the response of S. paramamosain to salinity stress. Crabs were transferred from a salinity of 25 ppt to salinities of 5 ppt or 33 pp… Show more

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Cited by 26 publications
(23 citation statements)
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References 85 publications
(120 reference statements)
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“…In the present study, a dozen of V-type H + -transporting ATPase genes (EVM0006836/ vha-1 , EVM0002567/ vha-3 , EVM0005735/ vha-4 , EVM0007934/ vha-5 , EVM0008894/ vha-5 , EVM0000966/ vha-7 , EVM0001861/ vha-8 , EVM0014072/ vha-12 , EVM0014618/ vha-13 , EVM0014647/ vha-15 , EVM0015095/ vha-16 , and EVM0006795/ vha-19 ) were significantly up-regulated under hyposaline condition ( Supplementary Figure 4 ). The upregulation of these genes was also reported in other marine invertebrates, including the mud crab Scylla paramamosain ( Niu et al, 2020 ) and the shrimp Litopenaeus vannamei ( Wang et al, 2012 ), indicating their conserved function in response to low salinity stress among marine invertebrates. On the other hand, a battery of ion channel and transporter genes such as potassium channel genes (EVM0012374/ twk-24 , EVM0015790/ shw-3 , EVM0004766/ kcnl-3 , and EVM0004207/ kcnl-2 ), sodium channel genes (EVM0009270/ egas-2 and EVM0008949/ nhx-8 ), cyclic nucleotide gated channel gene (EVM0006320/ tax-4 ), potassium/chloride transporter gene (EVM0004010/ kcc-2 ), and transient receptor potential cation channel genes (EVM0012432/ trp-1 , EVM0001337/ trp-2 , and EVM0005441/ osm-9 ) were significantly down-regulated in both stress environments ( Figure 3G ), reflecting their association with the ionic homeostasis under salinity stresses.…”
Section: Resultsmentioning
confidence: 53%
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“…In the present study, a dozen of V-type H + -transporting ATPase genes (EVM0006836/ vha-1 , EVM0002567/ vha-3 , EVM0005735/ vha-4 , EVM0007934/ vha-5 , EVM0008894/ vha-5 , EVM0000966/ vha-7 , EVM0001861/ vha-8 , EVM0014072/ vha-12 , EVM0014618/ vha-13 , EVM0014647/ vha-15 , EVM0015095/ vha-16 , and EVM0006795/ vha-19 ) were significantly up-regulated under hyposaline condition ( Supplementary Figure 4 ). The upregulation of these genes was also reported in other marine invertebrates, including the mud crab Scylla paramamosain ( Niu et al, 2020 ) and the shrimp Litopenaeus vannamei ( Wang et al, 2012 ), indicating their conserved function in response to low salinity stress among marine invertebrates. On the other hand, a battery of ion channel and transporter genes such as potassium channel genes (EVM0012374/ twk-24 , EVM0015790/ shw-3 , EVM0004766/ kcnl-3 , and EVM0004207/ kcnl-2 ), sodium channel genes (EVM0009270/ egas-2 and EVM0008949/ nhx-8 ), cyclic nucleotide gated channel gene (EVM0006320/ tax-4 ), potassium/chloride transporter gene (EVM0004010/ kcc-2 ), and transient receptor potential cation channel genes (EVM0012432/ trp-1 , EVM0001337/ trp-2 , and EVM0005441/ osm-9 ) were significantly down-regulated in both stress environments ( Figure 3G ), reflecting their association with the ionic homeostasis under salinity stresses.…”
Section: Resultsmentioning
confidence: 53%
“…Additionally, based on studies on fishes and marine invertebrates, ion transporters and channels are key components of osmoregulation ( Niu et al, 2020 ; Vij et al, 2020 ; Zhang Y. et al, 2020 ). In the present study, a dozen of V-type H + -transporting ATPase genes (EVM0006836/ vha-1 , EVM0002567/ vha-3 , EVM0005735/ vha-4 , EVM0007934/ vha-5 , EVM0008894/ vha-5 , EVM0000966/ vha-7 , EVM0001861/ vha-8 , EVM0014072/ vha-12 , EVM0014618/ vha-13 , EVM0014647/ vha-15 , EVM0015095/ vha-16 , and EVM0006795/ vha-19 ) were significantly up-regulated under hyposaline condition ( Supplementary Figure 4 ).…”
Section: Resultsmentioning
confidence: 99%
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“…In addition to the final physiological acclimation to external salinity, there are also phenotypic differences in behavior and body morphology or size between marine populations and freshwater populations as reported in Fundulus and threespine sticklebacks (Kültz et al, 2015;Styga et al, 2019). Similar regulatory mechanisms have also been reported in crustaceans, like crabs and shrimps (Thabet et al, 2017;Rahi et al, 2019;Niu et al, 2020). However, the precise mechanisms underlying osmotic sensation, signal transduction and adaptation remain elusive.…”
Section: Discussionmentioning
confidence: 87%
“…There are a number of organic osmolytes such as glycerol, trehalose, inositol, betaine and taurine, allow cells to against effects of hyperosmolarity and to adapt to hyperosmotic conditions. For example, free amino acids and methylamines are mainly utilized by most marine invertebrates (Thabet et al, 2017;Niu et al, 2020), whereas glycerol is the most important osmolyte for the terrestrial nematode C. elegans (Choe, 2013;Urso and Lamitina, 2021). It is well documented that an increased level of glycerol is essential for C. elegans' survival in hypertonic environments mediated by upregulation of the glycerol biosynthetic enzyme gene gpdh-1 (Lamitina et al, 2006;Urso and Lamitina, 2021).…”
Section: Accumulation Of Organic Osmolytes Is Required For Both Early Salinity Stress Response and Long-term Salinity Acclimation In L Mamentioning
confidence: 99%