Multi-omic characterisation of<i>Streptomyces hygroscopicus</i>NRRL 30439: detailed assessment of its secondary metabolic potential

Barry, Craig; Gillane, Rosemary; Talbo, Gert; Plan, Manuel R.; Palfreyman, Robin W.; Haber-Stuk, Andrea; Power, J. B.; Nielsen, Lars K.; Marcellin, Esteban

doi:10.1039/d1mo00150g

Cited by 6 publications

(2 citation statements)

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“…Multi-omics research technology is of great signi cance for the screening of key genes. By mining relevant functional genes, analyzing the main growth-promoting characteristics of different strains, and deepening the understanding of the life activities of various strains at the molecular level, it can provide theoretical support for strengthening the research of the growth-promoting characteristics of various strains, improving the level of salt stress resistance of plants and the metabolic complementation of strains [21].…”

Section: Introductionmentioning

confidence: 99%

Study on the mechanism of salt relief and growth promotion of Enterobacter cloacae on cotton

Yue,

Sun,

et al. 2023

Preprint

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Aims In-depth studies on plant ion uptake and plant growth-promoting rhizobacteria(PGPR) at the molecular level will help to further reveal the effects of PGPR on plants and their interaction mechanisms under salt stress. Methods Cotton was inoculated with a PGPR-Enterobacter cloacae Rs-35, and the ion uptake capacity, membrane transporter protein activity, and expression of key genes were determined under salt stress. Changes in the endogenous hormone content of cotton were also determined. Further, the genome-wide metabolic pathway annotation of E. cloacae Rs-35 and its differential enrichment pathway analysis of multi-omics under salinity environments were performed. Results In a pot experiment of saline-alkali soil, E. cloacae Rs-35-treated cotton significantly increased its uptake of K+ and Ca2+ and decreased uptake of Na+, elevated the activity of the H+-ATPase, and increased the sensitivity of the Na+/H+ reverse transporter protein on the vesicle membrane. Meanwhile, inoculation with E. cloacae Rs-35 could promote cotton maintain the indoleacetic acid(IAA) content under salt stress. Genome-wide annotation showed that E. cloacae Rs-35 was respectively annotated to 31, 38, and 130 related genes in osmotic stress, phytohormone and organic acid metabolism, and ion uptake metabolic pathway. Multi-omics differences analysis showed that E. cloacae Rs-35 were enriched to tryptophan metabolism, multiple amino acid biosynthesis, carbon and glucose synthesis, and oxidative phosphorylation metabolic pathways at the transcriptome, proteome, and metabolome. Conclusion E. cloacae Rs-35 can promote cotton balance cell ion concentration, stabilize intracellular IAA changes, stimulate induction of systemic tolerance, and promotes the growth of cotton plants under salt stress.

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Section: Introductionmentioning

confidence: 99%

Study on the mechanism of salt relief and growth promotion of Enterobacter cloacae on cotton

Yue,

Sun,

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Multi-omics research technology is of great significance for the screening of key genes. By mining relevant functional genes, analyzing the main growth-promoting characteristics of different strains, and deepening the understanding of the life activities of various strains at the molecular level, it can provide theoretical support for strengthening the research of the growth-promoting characteristics of various strains, improving the level of salt stress resistance of plants and the metabolic complementation of strains [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Study on the mechanism of salt relief and growth promotion of Enterobacter cloacae on cotton

Yue,

Sun,

Wang

et al. 2023

BMC Plant Biol

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Aims In-depth studies on plant ion uptake and plant growth-promoting rhizobacteria (PGPR) at the molecular level will help to further reveal the effects of PGPR on plants and their interaction mechanisms under salt stress. Methods Cotton was inoculated with a PGPR-Enterobacter cloacae Rs-35, and the ion uptake capacity, membrane transporter protein activity, and expression of key genes were determined under salt stress. Changes in the endogenous hormone content of cotton were also determined. Further, the genome-wide metabolic pathway annotation of E. cloacae Rs-35 and its differential enrichment pathway analysis of multi-omics under salinity environments were performed. Results In a pot experiment of saline-alkali soil, E. cloacae Rs-35-treated cotton significantly increased its uptake of K+ and Ca2+ and decreased uptake of Na+, elevated the activity of the H+-ATPase, and increased the sensitivity of the Na+/H+ reverse transporter protein on the vesicle membrane. Meanwhile, inoculation with E. cloacae Rs-35 could promote cotton to maintain the indole-3-acetic acid (IAA) content under salt stress. Genome-wide annotation showed that E. cloacae Rs-35 was respectively annotated to 31, 38, and 130 related genes in osmotic stress, phytohormone and organic acid metabolism, and ion uptake metabolic pathway. Multi-omics differences analysis showed that E. cloacae Rs-35 were enriched to tryptophan metabolism, multiple amino acid biosynthesis, carbon and glucose synthesis, and oxidative phosphorylation metabolic pathways at the transcriptome, proteome, and metabolome. Conclusion E. cloacae Rs-35 can promote cotton balance cell ion concentration, stabilize intracellular IAA changes, stimulate induction of systemic tolerance, and promote the growth of cotton plants under salt stress.

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An extended catalogue of ncRNAs in Streptomyces coelicolor reporting abundant tmRNA, RNase-P RNA and RNA fragments derived from pre-ribosomal RNA leader sequences

Arancio

Genovese²,

Benfante

et al. 2022

Arch Microbiol

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Multi-omic characterisation ofStreptomyces hygroscopicusNRRL 30439: detailed assessment of its secondary metabolic potential

Abstract: The emergence of multidrug-resistant pathogenic bacteria creates a demand for novel antibiotics with distinct mechanisms of action. Advances in next-generation genome sequencing promised a paradigm shift in the quest to...

Cited by 6 publications

References 75 publications

Study on the mechanism of salt relief and growth promotion of Enterobacter cloacae on cotton

Study on the mechanism of salt relief and growth promotion of Enterobacter cloacae on cotton

Study on the mechanism of salt relief and growth promotion of Enterobacter cloacae on cotton

An extended catalogue of ncRNAs in Streptomyces coelicolor reporting abundant tmRNA, RNase-P RNA and RNA fragments derived from pre-ribosomal RNA leader sequences

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