Multi-omic profiling to assess the effect of iron starvation inStreptococcus pneumoniaeTIGR4

Abstract

We applied multi-omics approaches (transcriptomics, proteomics and metabolomics) to study the effect of iron starvation on the Gram-positive human pathogen Streptococcus pneumoniae to elucidate global changes in the bacterium in a condition similar to what can be found in the host during an infectious episode. We treated the reference strain TIGR4 with the iron chelator deferoxamine mesylate. DNA microarrays revealed changes in the expression of operons involved in multiple biological processes, with a prevalence of genes coding for ion binding proteins. We also studied the changes in protein abundance by 2-DE followed by MALDI-TOF/TOF analysis of total cell extracts and secretome fractions. The main proteomic changes were found in proteins related to the primary and amino sugar metabolism, especially in enzymes with divalent cations as cofactors. Finally, the metabolomic analysis of intracellular metabolites showed altered levels of amino sugars involved in the cell wall peptidoglycan metabolism. This work shows the utility of multi-perspective studies that can provide complementary results for the comprehension of how a given condition can influence global physiological changes in microorganisms.

IurV, Encoded by ORF VCA0231, Is Involved in the Regulation of Iron Uptake Genes in Vibrio cholerae

“…Together with iron, riboflavin-derived molecules are the most important redox cofactors in enzymatic reactions [ 67 ]. Probably owing to this common property, iron and riboflavin metabolism has been reported to be highly related in some bacteria [ 37 , 38 , 68 , 69 , 70 , 71 , 72 ]. In V. cholerae , we have documented that riboflavin and iron reciprocally regulate their provision systems [ 38 ].…”

Proteomic profiling analysis of postmenopausal osteoporosis and osteopenia identifies potential proteins associated with low bone mineral density

“…When compared with the findings of a genome and transcriptome study, proteomics and metabolomics appears to be closer to the phenotype, so it is more suitable for mechanistic studies, disease typing, and biomarker discovery (Jimenez-Munguia et al, 2018;Li & Shui, 2019;Wilmanski et al, 2019). Previous human studies have identified a few candidate metabolites for BMD alteration (Miyamoto et al, 2018;You et al, 2014), while more research about proteome studies is needed to better understand the molecular changes associated with low BMD in OP and ON, especially in postmenopausal women who are more susceptible to experiencing fragility fractures.…”

Peer Review #3 of "Proteomic profiling analysis of postmenopausal osteoporosis and osteopenia identifies potential proteins associated with low bone mineral density (v0.1)"

“…When compared with the findings of a genome and transcriptome study, proteomics and metabolomics appears to be closer to the phenotype, so it is more suitable for mechanistic studies, disease typing, and biomarker discovery (Jimenez-Munguia et al, 2018;Li & Shui 2019;Wilmanski et al, 2019). Previous human studies have identified a few candidate metabolites for BMD alteration (Miyamoto et al, 2018;You et al, 2014), while more research about proteome studies is needed to better understand the molecular changes associated with low BMD in OP and ON, especially in postmenopausal women who are more susceptible to experiencing fragility fractures.…”