Multi-Omics Analysis of Anti-Inflammatory Action of Alkaline Extract of the Leaves of Sasa sp.

Sakagami, Hiroshi; Nakatani, Sachie; Enomoto, Ayame; Ota, Sana; Kaneko, Miku; Sugimoto, Masahiro; Horiuchi, Misaki; Toeda, Kazuki; Oizumi, Takaaki

doi:10.3390/jcm10102100

Cited by 8 publications

(10 citation statements)

References 32 publications

(36 reference statements)

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“…Alkaline extract of Sasa sp. inhibited IL-1 β -induced prostaglandin E2 in human gingival fibroblasts [ 25 ]. Kim et al prepared a cellular inflammatory bowel disease (IBD) model with coculture of human intestinal epithelial Caco-2 cells and RAW264.7 cells [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Cellular Anti-Inflammatory and Antioxidant Activities of Bamboo Sasa albomarginata Leaf Extract and Its Constituent Coumaric Acid Methyl Ester

Kojima

Hakamata

Asanuma

et al. 2022

The Scientific World Journal

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Background. Hot water extract of Sasa albomarginata (Kumazasa) leaves is commercially available and used as a dietary supplement or skincare cream. The extract possesses anti-inflammatory activity on the mouse atopic dermatitis model. To elucidate the mechanism of in vivo activity, we have studied the cellular anti-inflammatory and antioxidant activities of the extract and its constituents. Methods. Secretion of mouse and human IL-6 was measured by ELISA. ROS production was measured by a fluorescent reagent. Ultrahigh performance liquid chromatography (UHPLC)/MS was used for the ingredient analysis. Results. The Sasa albomarginata extract inhibited inflammatory mediators such as LPS-induced NO, IL-6, and ROS production in mouse monocyte leukemia RAW264.7 cells. It also inhibited iNOS, IL-6, and IL-1β expressions. Moreover, it inhibited LPS-induced IL-6 expression and production in human monocyte leukemia THP-1 cells differentiated into macrophages. The HPLC analysis of the extract revealed the existence of coumaric acid, ferulic acid, and coumaric acid methyl ester. Coumaric acid methyl ester but not coumaric acid or ferulic acid inhibited LPS-induced NO, IL-6, and ROS production in RAW264.7 cells and IL-6 production in differentiated THP-1 cells. Conclusion. The hot water extract of Sasa albomarginata leaves and one of its constituents possess cellular anti-inflammatory and antioxidant activities.

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Section: Discussionmentioning

confidence: 99%

Cellular Anti-Inflammatory and Antioxidant Activities of Bamboo Sasa albomarginata Leaf Extract and Its Constituent Coumaric Acid Methyl Ester

Kojima

Hakamata

Asanuma

et al. 2022

The Scientific World Journal

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“…On the other hand, alkaline extract such as SE showed slightly higher anti-UVC activity. This is possibly due to the presence of p-coumaric acid (degradation product of lignin) identified by metabolomics analysis (20).…”

Section: Discussionmentioning

confidence: 99%

“…of the leaves of Sasa sp. (SE) (20) was provided by Daiwa Biological Research Institute Co., Ltd. (Tokyo, Japan). Lastly, 96microwell plates were from Techno Plastic Products AG (Trasadingen, Switzerland).…”

Section: Figure 1 Experimental Procedures Of Uvc Irradiation (A) and ...mentioning

confidence: 99%

Prominent Anti-UVC Activity of Lignin Degradation Products

Sakagami

Amano

Uota

et al. 2022

In Vivo

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Background/Aim: The rapid spread of COVID-19 resulted in the revision of the value of ultraviolet C (UVC) sterilization in working spaces. This study aimed at re-evaluating the anti-UVC activity of four groups of natural products against human melanoma COLO679 and human normal dermal fibroblast (HDFa) cells, based on chemotherapeutic index. Materials and Methods: Various cell lines were exposed to UVC for 3 min in the presence of increasing concentrations of test compounds and viable cell numbers were determined with the 3-( 4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay. The anti-UVC activity was quantified by the ratio of the 50% cytotoxic concentration (determined without irradiation) to the 50% effective concentration (which abolished by 50% the UVCinduced loss of viability). Apoptosis was quantified as the subG 1 population proportion following cell-cycle analysis. Results: Among four groups of major natural products, six phenylpropanoids showed the highest anti-UVC activity, followed by the lignified products and alkaline products that contain lignin and its degradation products. On the other hand, tannins and flavonoids showed lower activity due to their higher cytotoxicity. UVC-sensitive COLO679 cells lack dectin-1 protein expression. Conclusion: These data suggest the prominent anti-UVC activity of lignin degradation products, and the possible involvement of dectin-1 expression in UVC-sensitivity.

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“…Pathogenic viruses such as HIV and HSV come with an array of complexities in the oral cavity, and therefore, keeping a clean mouth is essential for living longer with a high QOL. Oral intake of SE significantly improved the symptoms of oral lichenoid dysplasia (as evidenced by the reduced area of white steaks and the decline of the salivary concentrations of interleukin-6 and -8) [ 47 ], possibly by its potent anti-inflammatory activity [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Lignosulfonate Rapidly Inactivates Human Immunodeficiency and Herpes Simplex Viruses

et al. 2021

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Very few studies of the antiviral potential of lignosulfonates have been published. With the aim of oral application, among various groups of natural products, the relative antiviral potency of lignosulfonate and its ability to rapidly inactivate viruses were investigated. Methods: As target cells, MT-4 cells in suspension and attached Vero cells were used for infections with human immunodeficiency virus (HIV) and human herpes simplex type-1 virus (HSV). Mock- or virus-infected cells were incubated for 3–5 days with various concentrations of test samples, and the viable cell number was determined with the MTT method. For the shorter exposure experiments, higher titers of HIV or HSV were exposed to test samples for 10 or 3 min, diluted to a normal multiplicity of infection (MOI), and applied to the cells. Antiviral activity was quantified by using the chemotherapy index. Results: In the long-exposure system, lignosulfonates showed comparable anti-HIV activity with those of AZT, ddC, and sulfated polysaccharides, and it exceeded those of hundreds of tannins and flavonoids. When the exposure time was shortened, the chemotherapeutic index of the lignosulfonates for HIV was increased 27-fold. At a physiological pH, lignosulfonate showed higher anti-HIV activity than commercial alkali-lignin, dealkali-lignin, and humic acid, possibly due to the higher solubility and purity. Conclusions: With their rapid virus-inactivation capabilities, lignosulfonates may be useful for the prevention or treatment of virally induced oral diseases.

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Multi-Omics Analysis of Anti-Inflammatory Action of Alkaline Extract of the Leaves of Sasa sp.

Cited by 8 publications

References 32 publications

Cellular Anti-Inflammatory and Antioxidant Activities of Bamboo Sasa albomarginata Leaf Extract and Its Constituent Coumaric Acid Methyl Ester

Cellular Anti-Inflammatory and Antioxidant Activities of Bamboo Sasa albomarginata Leaf Extract and Its Constituent Coumaric Acid Methyl Ester

Prominent Anti-UVC Activity of Lignin Degradation Products

Lignosulfonate Rapidly Inactivates Human Immunodeficiency and Herpes Simplex Viruses

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