Multi-omics analysis of magnetically levitated plasma biomolecules

Ashkarran, Ali Akbar; Gharibi, Hassan; Zeki, Dalia Abou; Radu, Irina; Khalighinejad, Farnaz; Keyhanian, Kiandokht; Abrahamsson, Christoffer; Ionete, Carolina; Saei, Amir Ata

doi:10.1016/j.bios.2022.114862

Cited by 2 publications

(2 citation statements)

References 60 publications

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“…Magnetic levitation (MagLev) is a new detection methodology in the biosensor field, which utilizes magnetic force to balance gravitational force and provide a density-based detection technique. − ,− MagLev-based sensor platforms were reported for the analysis of biological and non-biological substances. MagLev-based sensor platforms are sensitive enough for protein assays, and they offer advantages such as adaptability, simplicity, and no need for sophisticated instruments. − In our previous studies, optimization of a MagLev-based methodology was carried out and the method demonstrated a lower protein detection limit of 4.1 ng/mL, which highlights the potential of it for various biosensing applications . In conclusion, MagLev-based sensors have the potential to be used in liquid biopsy, thus providing a new frontier for innovative methodologies for cancer diagnosis.…”

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confidence: 92%

Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes

Sözmen,

Arslan-Yildiz

2024

ACS Sens.

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Extracellular vesicles, especially exosomes, have attracted attention in the last few decades as novel cancer biomarkers. Exosomal membrane proteins provide easy-to-reach targets and can be utilized as information sources of their parent cells. In this study, a MagLev-based, highly sensitive, and versatile biosensor platform for detecting minor differences in the density of suspended objects is proposed for exosome detection. The developed platform utilizes antibody-functionalized microspheres to capture exosomal membrane proteins (ExoMPs) EpCAM, CD81, and CD151 as markers for cancerous exosomes, exosomes, and non-small cell lung cancer (NSCLC)-derived exosomes, respectively. Initially, the platform was utilized for protein detection and quantification by targeting solubilized ExoMPs, and a dynamic range of 1−100 nM, with LoD values of 1.324, 0.638, and 0.722 nM for EpCAM, CD81, and CD151, were observed, respectively. Then, the sensor platform was tested using exosome isolates derived from NSCLC cell line A549 and MRC5 healthy lung fibroblast cell line. It was shown that the sensor platform is able to detect and differentiate exosomal biomarkers derived from cancerous and non-cancerous cell lines. Overall, this innovative, simple, and rapid method shows great potential for the early diagnosis of lung cancer through exosomal biomarker detection.

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confidence: 92%

Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes

Sözmen,

Arslan-Yildiz

2024

ACS Sens.

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“…Slightly larger objects than a few micrometers take less time, but a significant time to separate (e.g. hours for ∼10 µm objects and around 30 min for ∼50 µm objects), mainly because their densities transform back from the effective to the bulk regime [18,19]. This limitation may prevent the practical application of MagLev in various areas (e.g.…”

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confidence: 99%

Magnetic levitation of nanoscale materials: the critical role of effective density

Ashkarran,

Mahmoudi

2023

J. Phys. D: Appl. Phys.

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The magnetic levitation (MagLev) of diamagnetic materials in a paramagnetic solution is a robust technique for the density-based separation, measurements, and analysis of bulk materials/objects (e.g., beads and plastics). There is a debate in the literature, however, about whether a MagLev technique is reliable for the separation and/or density measurements of nanoscale objects. Here, we show that MagLev can levitate nanoparticles; however, the transition from the bulk to an ‘effective’ density must be acknowledged and considered in density measurements at the nanoscale regime. We performed a proof-of-concept study on MagLev’s capability in measuring the ‘effective density’ of multiscale silver particles (i.e. microparticles, nanopowder, and nanoemulsion). In addition, we probed the effective density of nanoscale biomolecules (e.g. lipoproteins) using a standard MagLev system. Our findings reveal that the MagLev technique has the capability to measure both bulk density (which is independent of the size and dimension of the material) and the effective density (which takes place at the nanoscale regime and is dependent on the size and surrounding paramagnetic solution) of the levitated objects.

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Multi-omics analysis of magnetically levitated plasma biomolecules

Cited by 2 publications

References 60 publications

Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes

Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes

Magnetic levitation of nanoscale materials: the critical role of effective density

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